Effect of cell density and nutrient deprivation on hydrogen production by unicellular green alga Scenedesmus sp. KMITL-OVG1

Main Article Content

Kittiphat Warichanan Saranya Phunpruch

Abstract

Hydrogen is considered as one of the energy carriers for the near future. H2 production by green algae is catalyzed by hydrogenase activity using electrons from photosynthetic process under the light and from accumulated carbohydrate catabolism in the dark. This research aimed to investigate the effect of cell density and nutrient deprivation on H2 production by Scenedesmus sp. KMITL-OVG1 isolated in Thailand. The result showed that cell culture with the optical density at 750 nm of 0.8 gave the highest H2 production rate. Interestingly, the highest H2 production rate of 1.957 ± 0.100 mL L-1 h-1 and hydrogenase activity of 0.031 ± 0.001 ml L-1 min-1 were found in cells incubated under potassium deprivation. H2 production rate was approximately 3 folds higher than that of cells incubated in normal TAP medium. The increased H2 production rate and hydrogenase activity might be involved in the reduction of starch accumulation. Moreover, the deprivation of potassium combined with other nutrients did not enhance H2 production rate by Scenedesmus sp. KMITL-OVG1.

Keywords

Article Details

Section
Research Articles

References

[1] Turner JA. A realizable renewable energy future. Science 1999;285:687-689.

[2] Gaffron H. Über auffallende Unterschiede in der Physiologie nahe verwandter Algenstämme, nebst Bemerkungen über der Lichtatmung. Biologisches Zentralblatt 1939;59:302-313.

[3] Gaffron H. The effect of specific poisons upon the photoreduction with hydrogen in green algae. Journal of General Physiology 1942;26:241-267.

[4] Gaffron H, Rubin J. Fermentative and photochemical production of hydrogen in algae. Journal of General Physiology 1942;26:219-240.

[5] Rattana S, Junyapoon S, Incharoensakdi A, Phunpruch S. Hydrogen production by the green alga Scenedesmus sp. KMITL-01 under heterotrophic conditions. 2010:Proceedings of the 8th International Symposium on Biocontrol and Biotechnology; 2010 Oct 4-6; Pattaya, Chonbuti, Thailand; 2010. p.114-120.

[6] Prince RC, Kheshgi HS. The photobiological production of hydrogen: potential efficiency and effectiveness as a renewable fuel. Critical Reviews in Microbiology 2005;31:19-31.

[7] Márquez-Reyes LA, Sánchez-Saavedra MDP, Valdez-Vazquez I. Improvement of hydrogen production by reduction of the photosynthetic oxygen in microalgae cultures of Chlamydomonas gloeopara and Scenedesmus obliquus. International Journal of Hydrogen Energy 2015;40:7291-7300.

[8] Forestier M, King P, Zhang L, Posewitz M, Schwarzer S, Happe T, Ghirardi ML, Seibert M. Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions. European Journal of Biochemistry 2003;270:2750-2758.

[9] Saleem M, Chakrabarti MH, Raman AAA, Daud WMAW, Mustafa A. Hydrogen production by Chlamydomonas reinhardtii in a two-stage process with and without illumination at alkaline pH. International Journal of Hydrogen Energy 2012;37:4930-4934.

[10] Davies JP, Yildiz FH, Grossman AR. Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO Journal 1996;15:2150-2159.

[11] Hase E, Morimura Y, Mihara S, Tamiya H. The role of sulfur in the cell division of Chlorella. Archives of Microbiology 1958;31:87- 95.

[12] Zhang L, Happe T, Melis A. Biochemical and morphological characterization of sulfur-deprived and H2- producing Chlamydomonas reinhardtii (green alga). Planta 2002;214:552-561.

[13] Wykoff DD, Davies JP, Melis A, Grossman AR. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiology 1998;117:129-139.

[14] Philipps G, Happe T, Hemschemeier A. Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii. Planta 2012;235:729-745.

[15] Evans HJ, Sorger GJ. Role of mineral elements with emphasis on the univalent cations. Annual Review of Plant Physiology 1966;17:47-76.

[16] Pongpadung P, Liu J, Yokthongwattana K, Techapinyawat S, Juntawong N. Screening for hydrogen-producing strains of green microalgae in phosphorus or sulphur deprived medium under nitrogen limitation. ScienceAsia 2015;41:97-107.

[17] Papazi A, Gjindali AI, Kastanaki E, Assimakopoulos K, Stamatakis, K, Kotzabasis K. Potassium deficiency, a “smart” cellular switch for sustained high yield hydrogen production by the green alga Scenedesmus obliquus. International Journal of Hydrogen Energy 2014;39:19452-19464.

[18] Batyrova KA, Tsygankov AA, Kosourov SN, Sustained hydrogen photoproduction by phosphorus deprived Chlamydomonas reinhardtii cultures. International Journal of Hydrogen Energy 2012;37:8834-8839.

[19] Richmond A. Open systems for the mass production of photoautotrophic microalgae outdoors - Physiological principles. Journal of Applied Phycology 1992;4:281-286.

[20] Wang Y, Wu WH. Potassium transport and signaling in higher plants. Annual Review of Plant Biology 2013;64:451-476.

[21] Hahn JJ, Ghirardi ML, Jacoby WA, Effect of process variables on photosynthetic algal hydrogen production. Biotechnology Progress 2004;20:989-991.

[22] Puangplub A, Incharoensakdi A, Phunpruch S. Screening of green algae isolated from natural water sources in Thailand for H2 production. 2017:Proceedings of the 55th Kasetsart University Annual Conference; 2017 Jan 31-Feb 3; Kasetsart University, Bangkok, Thailand; 2017. p. 199-206.

[23] Harris EH. The Chlamydomonas sourcebook: A comprehensive guide to biology and laboratory use, ed. San Diego, Academic Press; 1989.

[24] Tinpranee N, Incharoensakdi A, Phunpruch S. Hydrogen production by unicellular green alga Chlorella sp. LSD-W2 isolated from seawater in Thailand. Asia-Pacific Journal of Science and Technology 2016;22(1):256-266.

[25] Taikhao S, Junyapoon S, Incharoensakdi A, Phunpruch S. Factors affecting biohydrogen production by unicellular halotolerant cyanobacterium Aphanothece halophytica. Journal of Applied Phycology 2013;25:575-585.

[26] Gfeller RP, Gibbs M, Fermentative metabolism of Chlamydomonas reinhardtii. I. Analysis of fermentative products from starch in dark and light. Plant physiology 1985;75:212-218.

[27] Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 1959;31:426-428.

[28] Kim JP, Kang CD, Sim SJ, Kim MS. Cell age optimization for hydrogen production induced by sulfur deprivation using a green alga Chlamydomonas reinhardtii utex 90. Journal of Microbiology and Biotechnology 2005;15(1):131-135.

[29] Ghirardi ML, Togasaki RK, Seibert M. Oxygen sensitivity of algal H2-production. Applied Biochemistry and Biotechnology 1997;63:141-151.

[30] Evans HJ, Sorger GJ. Role of mineral elements with emphasis on the univalent cations. Annual Review of Plant Physiology 1966;17:47-76.

[31] Iyer G, Gupte Y, Vaval P, Nagle V. Uptake of potassium by algae and potential use as biofertilizer. Indian Journal of Plant Physiology 2015;20(3):285-288.

[32] Oncel SS, Kose A, Faraloni C, Imamoglu E, Elibol M, Torzillo G. Biohydrogen production using mutant strains of Chlamydomonas reinhardtii: the effects of light intensity and illumination patterns. Biochemical Engineering Journal 2014;92:47-52.

[33] Edelman, M., Mattoo, AK., Marder JB., 1984. Three hats of the rapidly metabolized 32 kD protein thylakoids. In Ellis, R.T. Ed. Chloroplast Biogenesis, ed. Cambridge University Press, Cambridge.

[34] Phunpruch S, Puangplub A, Incharoensakdi A. Biohydrogen production by microalgae isolated from the rice paddle field in Thailand. Asia-Pacific Journal of Science and Technology 2016;22(1):236-247.

[35] Papazi A, Andronis E, Ioannidis NE, Chaniotakis N, Kotzabasis K. High yields of hydrogen production induced by meta-substituted dichlorophenols biodegradation from the green alga Scenedesmus obliquus. Public Library of Science 2012;7(11):e49037.