Identification and Characterization of Candida tropicalis Isolated from Soil of Sugarcane Plantation in Thailand for Ethanol Production
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Published:
May 18, 2018
Keywords:
Candida tropicalis thermotolerant ethanol tolerant ethanol production
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Abstract
The thermotolerant and ethanol-producing yeasts are especially required in numerous industrial applications, such as alternative sources for bioethanol. In this study, we isolated 12 novel thermotolerant yeast strains using the enrichment technique with 4% (v/v) ethanol. The growth phenotype and ethanol fermentation activity under stress conditions were compared with the Thai industrial strain Saccharomyces cerevisiae TISTR5339. All isolated strains showed their ability to grow at 42°C. Among them, five isolates were found to be tolerant up to 20% (v/v) ethanol and six isolates demonstrated higher tolerance both to high temperature and ethanol up to 40°C with 10% (v/v) ethanol. The specific growth rates (µ) at high temperature of all strains ranged between 0.470-0.577 (h-1) at 40°C, which were greater than that of the reference strain. Especially one strain in this study, the strain KPC6, could convert glucose to ethanol at 14.422 g/L in 12 h and 45.232 g/L in 24 h, which showed higher productivity of ethanol than the reference strain at 42°C. The phylogenetic analysis based on the sequences of D1/D2 domain of 26S rDNA revealed that all strains were belonging to Candida tropicalis. Considering the results in this study, it is suggested that the Thai C. tropicalis KPC strains isolated in this study are thermo- and ethanol-tolerant ones which can be utilized as industrial microorganisms with traits that are important for future adaptation in industrial ethanol production.
Article Details
How to Cite
Pongcharoen, P., & Miyuki, K.-K. (2018). Identification and Characterization of Candida tropicalis Isolated from Soil of Sugarcane Plantation in Thailand for Ethanol Production. Asia-Pacific Journal of Science and Technology, 23(3), APST–23. https://doi.org/10.14456/apst.2018.3
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Research Articles
References
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[26] Limtong S, Srisuk N, Yongmanitchai W, Kawasaki H, Yurimoto H, Nakase T, Kato N., 2004. Three new thermotolerant methylotrophic yeasts, Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolated in Thailand. Journal of General and Applied Microbiology 50, 119-127.
[27] Limtong S, Srisuk N, Yongmanitchai W, Yurimoto H, Nakase T, Kato N., 2005. Pichia thermomethanolica sp. nov., a novel thermotolerant, methylotrophic yeast isolated in Thailand. International Journal of Systematic and Evolutionary Microbiology 55, 2225-2229.
[28] Buddiwong S, Thanonkeo S, Phetsom J, Jaisil P, Thanonkeo P., 2014. Screening of thermotolerant yeast isolated from sugarcane plantations in Northeastern part of Thailand. KKU Research Journal 19, 217-223.
[29] Wu WH, Hung WC, Lo KY, Chen YH, Wan HP, Cheng KC., 2016. Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21. Bioresource Technology 201, 27-32.
[30] Della-Bianca BE, Gombert AK., 2013. Stress tolerance and growth physiology of yeast strains from the Brazilian fuel ethanol industry. Antonie Van Leeuwenhoek 104, 1083-1095.
[2] Hari Krishna S, Janardhan Reddy T, Chowdary GV., 2001. Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast. Bioresource Technology 77, 193-196.
[3] Abdel-Banat BM, Hoshida H, Ano A, Nonklang S, Akada R., 2010. High-temperature fermentation: How can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? Applied Microbiology and Biotechnology 85, 861-867.
[4] Dung NT, Rombouts FM, Nout MJ., 2006. Functionality of selected strains of moulds and yeasts from Vietnamese rice wine starters. Food Microbiology 23, 331-340.
[5] Hahn-Hägerdal B1, Galbe M, Gorwa-Grauslund MF, . 2006. Lidén G, Zacchi G. Bio-ethanol - the fuel of tomorrow from the residues of today. Trends Biotechnology 24, 549-556.
[6] Benjaphokee S, Koedrith P, Auesukaree C, Asvarak T, Sugiyama M, Kaneko Y, Boonchird C, Harashima S., 2012. CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae. New Biotechnology 29, 166-176.
[7] Auesukaree C, Koedrith P, Saenpayavai P, Asvarak T, Benjaphokee S, Sugiyama M, KanekoY, Harashima S, Boonchird C., 2012. Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. Journal of Bioscience and Bioengineering 114, 144-149.
[8] Kaewkrajay C, Dethoup T, Limtong S., 2014. Ethanol production from cassava using a newly isolated thermotolerant yeast strain. ScienceAsia 40, 268-277.
[9] Nitiyon S, Boonmak C, Am-In S, Jindamorakot S, Kawasaki H, Yongmanitchai W, Limtong S., 2011. Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., xylose-utilizing yeast species isolated in Thailand. International Journal of Systematic and Evolutionary Microbiology 61, 462-468.
[10] Yuangsaard N, Yongmanitchai W, Yamada M, Limtong S., 2013. Selection and characterization of a newly isolated thermotolerant Pichia kudriavzevii strain for ethanol production at high temperature from cassava starch hydrolysate. Antonie Van Leeuwenhoek 103, 577-588.
[11] Kuntiya A, Takenaka S, Seesuriyachan P., 2013. High potential of thermotolerant Candida tropicalis No.10 for high concentration of phenol biodegradation. Food and Applied Bioscience Journal 1(2), 59-68.
[12] Jamai L, Ettayebi K, El Yamani J, Ettayebi M., 2007. Production of ethanol from starch by free and immobilized Candida tropicalis in the presence of α-amylase. Bioresour Technology 98, 2765-2770.
[13] Nitiyon S, Keo-Oudone C, Murata M, Lertwattanasakul N, Limtong S, Kosaka T, Yamada M., 2016. Efficient conversion of xylose to ethanol by stress-tolerant Kluyveromyces marxianus BUNL-21. SpringerPlus 5, 1-12.
[14] Lorliam W, Akaracharanya A, Jindamorakot S, Suwannarangsee S, Tanasupawat S., 2013. Characterization of xylose-utilizing yeasts isolated from herbivore faeces in Thailand. ScienceAsia 39, 26-35.
[15] Okonechnikov K, Golosova O, Fursov M, Varlamov A, Vaskin Y, Efremov I, German Grehov O.G., Kandrov D, Rasputin K, Syabro M, Tleukenov T., 2012. Unipro UGENE: A unified bioinformatics toolkit. Bioinformatics 28, 1166-1167.
[16] Thompson JD, Higgins DG, Gibson TJ., 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.
[17] Kumar S, Stecher, Tamura K., 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33, 1870-1874.
[18] Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O., 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59, 307-321.
[19] Banat IM, Nigam P, Singh D, Marchant R, McHale A., 1998. Ethanol production at elevated temperatures and alcohol concentrations: a review. Part I – yeast in general. World Journal of Microbiology and Biotechnology 14, 809-821.
[20] Kiran Sree N, Sridhar M, Suresh K, Banat IM, Venkateswar Rao L., 2000. Isolation of thermotolerant, osmotolerant, flocculating Saccharomyces cerevisiae for ethanol production. Bioresource Technology 72, 43-46.
[21] Limtong S, Sringiew C, Yongmanitchai W., 2007. Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresource Technology 98, 3367-3374.
[22] Koedrith P, Dubois E, Scherens B, Jacobs E, Boonchird C, Messenguy F., 2008. Identification and characterization of a thermotolerant yeast strain isolated from banana leaves. ScienceAsia 34, 147-152.
[23] Ma M, Liu LZ., 2010. Quantitative transcription dynamic analysis reveals candidate genes and key regulators for ethanol tolerance in Saccharomyces cerevisiae. BMC Microbiology 10, 1-20.
[24] Zakrzewska A, van Eikenhorst G, Burggraaff JE, Vis DJ, Hoefsloot H, Delneri D, Oliver SG, Brul S, Smits GJ., 2011. Genome-wide analysis of yeast stress survival and tolerance acquisition to analyze the central trade-off between growth rate and cellular robustness. Molecular Biology of the Cell 22, 4435-4446.
[25] Versele M, Thevelein JM, Van Dijck P., 2004. The high general stress resistance of the Saccharomyces cerevisiae fil1 adenylate cyclase mutant (Cyr1Lys1682) is only partially dependent on trehalose, Hsp104 and overexpression of Msn2/4-regulated genes. Yeast 21, 75-86.
[26] Limtong S, Srisuk N, Yongmanitchai W, Kawasaki H, Yurimoto H, Nakase T, Kato N., 2004. Three new thermotolerant methylotrophic yeasts, Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolated in Thailand. Journal of General and Applied Microbiology 50, 119-127.
[27] Limtong S, Srisuk N, Yongmanitchai W, Yurimoto H, Nakase T, Kato N., 2005. Pichia thermomethanolica sp. nov., a novel thermotolerant, methylotrophic yeast isolated in Thailand. International Journal of Systematic and Evolutionary Microbiology 55, 2225-2229.
[28] Buddiwong S, Thanonkeo S, Phetsom J, Jaisil P, Thanonkeo P., 2014. Screening of thermotolerant yeast isolated from sugarcane plantations in Northeastern part of Thailand. KKU Research Journal 19, 217-223.
[29] Wu WH, Hung WC, Lo KY, Chen YH, Wan HP, Cheng KC., 2016. Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21. Bioresource Technology 201, 27-32.
[30] Della-Bianca BE, Gombert AK., 2013. Stress tolerance and growth physiology of yeast strains from the Brazilian fuel ethanol industry. Antonie Van Leeuwenhoek 104, 1083-1095.