The Effect of Different Feed Types on the Growth Rate and Biochemical Composition of the Marine ciliate, Euplotes sp.

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Woraporn Tarangkoon
Nopparat Mahae
Suwat Tanyaros

Abstract

This research determined and compared growth rates and chemical composition of the marine ciliate, Euplotes sp. cultured with different diets under laboratory conditions. The different diets consisted of algae (Isochrysis galbana, Tetraselmis suecica), baker’s yeast (Saccharomyces cerevisiae) and marine thraustochytrid, Aurantiochytrium limacinum, assigned as mono- or mixed diets with an initial concentration of 60,000 cells.ml-1. Twenty single-cell Euplotes sp. were cultured in each treatment. Results revealed that growth rates of Euplotes sp. varied significantly (p<0.05) among different feed types. There were no significant differences for the highest mean growth rates of Euplotes sp. fed on the monoalgal diet T. suecica and the mixed diet consisting of T. suecica, S. cerevisiae and A. limacinum. Examination of the biochemical composition of Euplotes sp. fed on the mono diet of S. cerevisiae and A. limacinum showed that oleic acid (C18:1n9c) was dominant in the S. cerevisiae group, while palmitic acid (C16:0) was dominant in the A. limacinum group. The A. limacinum diet contained docosahexaenoic acid (C22:6n-3, DHA), but only a small percentage of this fatty acid was found in the ciliates fed with this diet. Eicosapentaenoic acid (C20:5n-3, EPA) was present in Euplotes sp. although it was not present in the A. limacinum diet. The amino acids cystine and tryptophan were present in the S. cerevisiae diet but were not detected in Euplotes sp. fed with it. Tryptophan was found in Euplotes sp. fed with the A. limacinum diet but was not found in the A. limacinum diet. The most abundant non-essential amino acids found in Euplotes sp. and in the assigned diets (S. cerevisiae and A. limacinum) were glutamic acid and serine.

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Tarangkoon, W., Mahae, N., & Tanyaros, S. (2018). The Effect of Different Feed Types on the Growth Rate and Biochemical Composition of the Marine ciliate, Euplotes sp. Journal of Fisheries and Environment, 42(1), 24–32. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/87072
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