Effect of Giant Bladder Kelp (Macrocystis pyrifera) Feed Additive on Growth, Survival and Immune Responses of Pacific White Shrimp (Litopenaeus vannamei) Injected with Vibrio parahaemolyticus

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Kanokwan Cherdkeattipol
Arunothai Keetanon
Niti Chuchird
Idsariya Wudtisin
Maximiliano López Frisbie
Albert G.J. Tacon

Abstract

A 60-day feeding trial was conducted with Pacific white shrimp (Litopenaeus vannamei) postlarvae 12 (PL12) to ascertain the effectiveness of using a giant bladder kelp as a feed additive. Shrimp growth, feed efficiency, survival, and immune response were evaluated. Postlarvae (PL) 12 were stocked in 16 × 500-L fiberglass tanks at a density of 50 PLs.tank-1. Water temperature and salinity were maintained at 29 ± 1 °C and 20-25 ppt, respectively. The shrimp were distributed into four groups (four replicates/treatment) and fed four times.day-1 with standard normal pelleted shrimp feed (control group) or feed mixed with 1, 2 and 3% by weight of the giant bladder kelp feed additive. The final weights and survival rates of shrimp were not significantly different among all treatments. However, shrimp raised on diets supplemented with giant bladder kelp showed significant improvement in their immune responses, including phenoloxidase and phagocytosis activities. The immune response of shrimp was significantly influenced by the percentage of the giant bladder kelp feed additive present in the shrimp feed. Shrimp fed with a diet containing 3% giant bladder kelp had significantly higher total hemocyte counts and percentages of phagocytosis, phenoloxidase, superoxide dismutase and bactericidal activities compared with the control group. Moroever, the survival rate of shrimp after challenging with Vibrio parahaemolyticus when fed diets containing 2 and 3% of giant bladder kelp were significantly increased compared with that of the control group. These findings demonstrated that the giant bladder kelp feed additive used in this study had a positive effect on the shrimp postlarval nonspecific immune responses and survival after being challenged with Vibrio parahaemolyticus.

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How to Cite
Cherdkeattipol, K., Keetanon, A., Chuchird, N., Wudtisin, I., Frisbie, M. L., & Tacon, A. G. (2017). Effect of Giant Bladder Kelp (Macrocystis pyrifera) Feed Additive on Growth, Survival and Immune Responses of Pacific White Shrimp (Litopenaeus vannamei) Injected with Vibrio parahaemolyticus. Journal of Fisheries and Environment, 41(3), 37–47. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/88097
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