Identification and Characterization of Potential Probiotic Bacillus spp. for Application in Striped Catfish (Pangasianodon hypophthalmus [Sauvage, 1878])

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Ho Thi Truong Thy
Nguyen Huu Thinh
Nguyen Nhu Tri
Ong Moc Quy
Korntip Kannika
Sasimamas Unajak
Nontawith Areechon

Abstract

One hudred and twenty isolates of Bacillus sp. were collected from the intestines of striped catfish (Pangasianodon hypophthalmus [Sauvage, 1878]) reared in farms in three provinces in the southern part of Vietnam: An Giang, Can Tho and Dong Thap. Isolates were identified by multiplex and single PCR using specific primers designed on gene 16S rRNA for six species of Bacillus (B.Subtilis, B. amyloliguefaciens, B. cereus, B. megaterium, B. licheniformis and B. pumilus). The in vitro analysis included inhibition activity against the pathogenic bacterium Edwardsiella ictaluri, protesase activity, stomach acidity and bile tolerance test, and antibiotic susceptibility. Results indicated that the highest number of isolate was B. megaterium and none was identified as B. licheniformis. The strain B. amyloliquefaciens 54A showed high protease activity and B. pumilus 47B showed high ingibition activity against E. ictaluri. These two probiotic candidates were also resistant to stomach acid pH and low bile salt conditions after 3 h and 24 h of exposure. Bacillus sp. candidates displayed varying sensitivities to the tested antibiotics with the exception of spectinomycin.

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How to Cite
Truong Thy, H. T., Thinh, N. H., Tri, N. N., Quy, O. M., Kannika, K., Unajak, S., & Areechon, N. (2017). Identification and Characterization of Potential Probiotic Bacillus spp. for Application in Striped Catfish (Pangasianodon hypophthalmus [Sauvage, 1878]). Journal of Fisheries and Environment, 41(2), 20–36. Retrieved from https://li01.tci-thaijo.org/index.php/JFE/article/view/92748
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