อิทธิพลของปัจจัยทางเคมีและทางกายภาพต่อการเพาะเลี้ยงยีสต์น้ำมันโดยใช้กลีเซอรอลดิบจากกระบวนการผลิตไบโอดีเซลเป็นแหล่งคาร์บอนหลัก

Main Article Content

Chutima Rakkitkanphun
Thanasak Lomthong
Apinan Wanlapa
Jantima Teeka
Atsadawut Areesirisuk

Abstract

The research aimed to study the effect of chemical and physical factors for cultivating oleaginous yeast Pseudozyma parantarctica CHC28 by using biodiesel-derived crude glycerol (BCG) as a sole carbon source. The seven variables (Glycerol concentration, (NH4)2SO4, KH2PO4, MgSO4, inoculum size, agitation rate, and temperature) were experimented and varied as two levels with Plackett-Burman experimental design (PBD). The maximum biomass, oil concentration, oil content, and oil production rate were obtained for 4.68 g/L, 2.53 g/L, 54.06% of dry biomass, and 0.84 g/L day, respectively. The results showed that yeast could utilize BCG for growing and producing microbial oil in all experiments. It demonstrated that the BCG could be used as a low-cost carbon source for cultivating P. parantarctica. Moreover, the experiment exhibited that glycerol concentration, MgSO4, and agitation rate were the first three highest contribution effect to biomass production for 49.46, 22.18, and 2.22%, respectively. While, glycerol concentration, KH2PO4 and agitation rate presented the first three highest contribution effect to oil concentration for 34.79, 11.22, and 10.73%, respectively. In addition, it was also observed that glycerol concentration, MgSO4, and agitation rate represented the positive effect on biomass and oil production while KH2PO4 provided a negative effect on oil production. This research demonstrated that BCG was an interesting inexpensive raw material for using as a carbon source of microbial oil production.

Article Details

Section
บทความวิจัย
Author Biographies

Chutima Rakkitkanphun, Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Thanasak Lomthong, Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Apinan Wanlapa, Division of Food Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi

Division of Food Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi

Jantima Teeka, Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi

Atsadawut Areesirisuk, Rajamangala University of Technology Thanyaburi

Division of Biology, Faculty of Science and Technology

Rajamangala University of Technology Thanyaburi

Rangsit-Nakornnayok Road, T. klong-Hok, A. Thanyaburi

Pathumthani Province, Thailand 12110

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