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The research aimed to study the effect of chemical and physical factors for cultivating oleaginous yeast Pseudozyma parantarctica CHC28 by using biodiesel-derived crude glycerol (BCG) as a sole carbon source. The seven variables (Glycerol concentration, (NH4)2SO4, KH2PO4, MgSO4, inoculum size, agitation rate, and temperature) were experimented and varied as two levels with Plackett-Burman experimental design (PBD). The maximum biomass, oil concentration, oil content, and oil production rate were obtained for 4.68 g/L, 2.53 g/L, 54.06% of dry biomass, and 0.84 g/L day, respectively. The results showed that yeast could utilize BCG for growing and producing microbial oil in all experiments. It demonstrated that the BCG could be used as a low-cost carbon source for cultivating P. parantarctica. Moreover, the experiment exhibited that glycerol concentration, MgSO4, and agitation rate were the first three highest contribution effect to biomass production for 49.46, 22.18, and 2.22%, respectively. While, glycerol concentration, KH2PO4 and agitation rate presented the first three highest contribution effect to oil concentration for 34.79, 11.22, and 10.73%, respectively. In addition, it was also observed that glycerol concentration, MgSO4, and agitation rate represented the positive effect on biomass and oil production while KH2PO4 provided a negative effect on oil production. This research demonstrated that BCG was an interesting inexpensive raw material for using as a carbon source of microbial oil production.
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