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Urinary tract infection is a common disease and a significant issue in the Thai public health system. Escherichia coli and Enterococcus faecalis are both major causes of urinary tract infection. Antibacterial resistance is always found in these two pathogens and could lead to difficulty in treatment and may even be life-threatening. The purpose of this study was to screen phytochemical constituents and antibacterial activity of the herbs in Benjapoltadhu, consisting of Cyperus alternifolius L., Homalomena aromatica (Spreng.) Schott, Cyperus rotundus L., Panicum repens L. and Kaempferia galanga L. Extraction of the herbs was carried out by boiling them in water and then freeze drying the extracts. The antimicrobial study was performed against 2 stains, E. coli and E. faecalis using the microdilution method. The results showed that the extract from C. alternifolius L. and H. aromatica (Spreng.) Schott could inhibit E. faecalis with MIC 62.5 and 100.0 mg/ml, respectively. Both herbs were found to contain groups of terpenoids, flavonoids, saponins and tannins. The results suggested that further study is required to elucidate the phytochemical content that possesses antibacterial properties for the development of new medicine in the future.
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