The Efficacy of 0.5 % Chlorhexidine Gluconate in 70 % Alcohol Compared with 10% Povidone Iodine in Reducing Blood Culture Contamination in Pediatric Patients

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Maliwan Sattabud


Background: Blood culture is the standard technique for diagnosis of septicemia but the incidence of bacterial contamination is high. This result is longer hospitalization and higher cost. To reduce contamination, antiseptics such as chlorhexidine and povidone iodine are generally used to clean the patients’ skin before taking blood.
Objectives: To evaluate the efficacy of 0.5 % chlorhexidine gluconate in 70 % alcohol compared with 10% povidone iodine in reducing blood culture contamination in pediatric patients.
Setting: Lahansai Hospital, Buriram provice.
Material and methods: This is a prospective study of pediatric patients who were admitted to Lahansai hospital from August 2016 to July 2017 .The subjects were provisionally diagnosed sepsis or bacteremia and blood culture were taken. The patients were classified into two groups according to antiseptic used: 10 % povidone iodine in odd months and 0.5 % chlorhexidine gluconate in 70 % alcohol in even months.
Results: This study include 364 blood culture specimens were collected.189 specimens used 10 % povidone iodine and 175specimens used 0.5 % chlorhexidine gluconate in 70 % alcohol as antiseptic. The results showed the 10 % povidone iodine group had 10.1 % bacterial contamination and 0.5 % chlorhexidine gluconate in 70 % alcohol group had 8.0 % bacterial contamination. However, these results are not statistically significant. The most common contaminated bacteria in the blood culture were Coagulase negative staphylococci (84.9 %).
Conclusion: For blood culture, use 0.5 % chlorhexidine gluconate in 70 % alcohol as septicemia showed lower bacterial contamination than use of 10 % Povidone Iodine.
Keywords: septicemia, Bacterial contamination.


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Original Articles


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