Hibiscus sabdariffa L. Extract Alleviates Vascular Endothelial Dysfunction by Enhancing Nitric Oxide Bioavailability in High-Fructose Diet Induced Insulin Resistance Rats
Keywords:
Hibiscus sabdariffa L.; insulin resistance; NO bioavailabilityAbstract
Background and Objectives: This study evaluated the effect of Hibiscus sabdariffa extract (HSE) on vascular endothelial function and mechanism involved in insulin resistance rats induced by high-fructose diet (HFD).
Methods: Male Sprague-Dawley rats were fed with HFD for 18 weeks to induce insulin resistance. Oral administration of HSE (200 or 500 mg/kg/day) was performed in the last four weeks. Vascular function and metabolic parameters were evaluated.
Results: HSE (200 and 500 mg/kg) improved glucose tolerance, reduced blood pressure, and restored vascular endothelial dysfunction in rats fed with HFD (p<0.05). Moreover, HSE 500 mg/kg enhanced nitric oxide bioavailability by restoring plasma NOx level and eNOS expression, and reducing O2•− production in insulin resistance rats (p<0.05).
Conclusion: Our results suggest that HSE alleviated vascular endothelial dysfunction in HFD fed rats that was associated with increasing in NO bioavailability.
References
1. Prabhakar P, Reeta KH, Maulik SK, Dinda AK, Gupta YK. Protective effect of thymoquinone against high-fructose diet-induced metabolic syndrome in rats. Eur J Nutr 2015; 54: 1117-27.
2. Shawky NM, Shehatou GS, Abdel Rahim M, Suddek GM, Gameil NM. Levocetirizine ameliorates high fructose diet-induced insulin resistance, vascular dysfunction and hepatic steatosis in rats. Eur J Pharmacol 2014; 740: 353-63.
3. Palanisamy N, Venkataraman AC. Beneficial effect of genistein on lowering blood pressure and kidney toxicity in fructose-fed hypertensive rats. Br J Nutr 2013; 109: 1806-12.
4. Peng CH, Chyau CC, Chan KC, Chan TH, Wang CJ, Huang CN. Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. J Agric Food Chem 2011; 59: 9901-9.
5. Ajiboye TO, Raji HO, Adeleye AO, Adigun NS, Giwa OB, Ojewuyi OB, et al. Hibiscus sabdariffa calyx palliates insulin resistance, hyperglycemia, dyslipidemia and oxidative rout in fructose-induced metabolic syndrome rats. J Sci Food Agric 2016; 96: 1522-31.
6. Sindi HA, Marshall LJ, Morgan MR. Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa. Food Chem 2014; 164: 23-9.
7. Hirunpanich V, Utaipat A, Morales NP, Bunyapraphatsara N, Sato H, Herunsale A, et al. Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats. J Ethnopharmacol 2006; 103: 252-60.
8. Bunbupha S, Pakdeechote P, Kukongviriyapan U, Pannangpetch P, Prachaney P, Berkban T, et al. Hibiscus sabdariffa extract alleviates insulin resistance and blood pressure in insulin resistance rats induced by a high fructose diet. The 11th Graduate Research Conferences KKU 2012: 549-57.
9. Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats. Plant Foods Hum Nutr 2007; 62: 1-6.
10. Suwannaphet W, Meeprom A, Yibchok-Anun S, Adisakwattana S. Preventive effect of grape seed extract against high-fructose diet-induced insulin resistance and oxidative stress in rats. Food Chem Toxicol 2010; 48: 1853-7.
11. Lu FJ, Lin JT, Wang HP, Huang WC. A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood. Experientia 1996; 52: 141-4.
12. Luangaram S, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Pannangpetch P. Protective effects of quercetin against phenylhydrazine-induced vascular dysfunction and oxidative stress in rats. Food Chem Toxicol 2007; 45: 448-55.
13. Verdon CP, Burton BA, Prior RL. Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the Griess reaction is used to assay for nitrite. Anal Biochem 1995; 224: 502-8.
14. Mukai Y, Sato S. Polyphenol-containing azuki bean (Vigna angularis) extract attenuates blood pressure elevation and modulates nitric oxide synthase and caveolin-1 expressions in rats with hypertension. Nutr Metab Cardiovasc Dis 2009; 19: 491-7.
15. Nakmareong S, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Kongyingyoes B, Donpunha W, et al. Tetrahydrocurcumin alleviates hypertension, aortic stiffening and oxidative stress in rats with nitric oxide deficiency. Hypertens Res 2012; 35: 418-25.
16. Katakam PV, Ujhelyi MR, Hoenig ME, Miller AW. Endothelial dysfunction precedes hypertension in diet-induced insulin resistance. Am J Physiol 1998; 275: R788-92.
17. Ajay M, Chai HJ, Mustafa AM, Gilani AH, Mustafa MR. Mechanisms of the anti-hypertensive effect of Hibiscus sabdariffa L. calyces. J Ethnopharmacol 2007; 109: 388-93.
18. Palmer RM, Ashton DS, Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 1988; 333: 664-6.
19. Glushakova O, Kosugi T, Roncal C, Mu W, Heinig M, Cirillo P, et al. Fructose induces the inflammatory molecule ICAM-1 in endothelial cells. J Am Soc Nephrol 2008; 19: 1712-20.
20. Potenza MA, Addabbo F, Montagnani M. Vascular actions of insulin with implications for endothelial dysfunction. Am J Physiol Endocrinol Metab 2009; 297: E568-77.
21. Hininger-Favier I, Benaraba R, Coves S, Anderson RA, Roussel AM. Green tea extract decreases oxidative stress and improves insulin sensitivity in an animal model of insulin resistance, the fructose-fed rat. J Am Coll Nutr 2009; 28: 355-61.
22. Farombi EO, Fakoya A. Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers of Hibiscus sabdariffa L. Mol Nutr Food Res 2005; 49: 1120-8.
23. Tseng TH, Kao ES, Chu CY, Chou FP, Lin Wu HW, Wang CJ. Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes. Food Chem Toxicol 1997; 35: 1159-64.
2. Shawky NM, Shehatou GS, Abdel Rahim M, Suddek GM, Gameil NM. Levocetirizine ameliorates high fructose diet-induced insulin resistance, vascular dysfunction and hepatic steatosis in rats. Eur J Pharmacol 2014; 740: 353-63.
3. Palanisamy N, Venkataraman AC. Beneficial effect of genistein on lowering blood pressure and kidney toxicity in fructose-fed hypertensive rats. Br J Nutr 2013; 109: 1806-12.
4. Peng CH, Chyau CC, Chan KC, Chan TH, Wang CJ, Huang CN. Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. J Agric Food Chem 2011; 59: 9901-9.
5. Ajiboye TO, Raji HO, Adeleye AO, Adigun NS, Giwa OB, Ojewuyi OB, et al. Hibiscus sabdariffa calyx palliates insulin resistance, hyperglycemia, dyslipidemia and oxidative rout in fructose-induced metabolic syndrome rats. J Sci Food Agric 2016; 96: 1522-31.
6. Sindi HA, Marshall LJ, Morgan MR. Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa. Food Chem 2014; 164: 23-9.
7. Hirunpanich V, Utaipat A, Morales NP, Bunyapraphatsara N, Sato H, Herunsale A, et al. Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats. J Ethnopharmacol 2006; 103: 252-60.
8. Bunbupha S, Pakdeechote P, Kukongviriyapan U, Pannangpetch P, Prachaney P, Berkban T, et al. Hibiscus sabdariffa extract alleviates insulin resistance and blood pressure in insulin resistance rats induced by a high fructose diet. The 11th Graduate Research Conferences KKU 2012: 549-57.
9. Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats. Plant Foods Hum Nutr 2007; 62: 1-6.
10. Suwannaphet W, Meeprom A, Yibchok-Anun S, Adisakwattana S. Preventive effect of grape seed extract against high-fructose diet-induced insulin resistance and oxidative stress in rats. Food Chem Toxicol 2010; 48: 1853-7.
11. Lu FJ, Lin JT, Wang HP, Huang WC. A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood. Experientia 1996; 52: 141-4.
12. Luangaram S, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Pannangpetch P. Protective effects of quercetin against phenylhydrazine-induced vascular dysfunction and oxidative stress in rats. Food Chem Toxicol 2007; 45: 448-55.
13. Verdon CP, Burton BA, Prior RL. Sample pretreatment with nitrate reductase and glucose-6-phosphate dehydrogenase quantitatively reduces nitrate while avoiding interference by NADP+ when the Griess reaction is used to assay for nitrite. Anal Biochem 1995; 224: 502-8.
14. Mukai Y, Sato S. Polyphenol-containing azuki bean (Vigna angularis) extract attenuates blood pressure elevation and modulates nitric oxide synthase and caveolin-1 expressions in rats with hypertension. Nutr Metab Cardiovasc Dis 2009; 19: 491-7.
15. Nakmareong S, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Kongyingyoes B, Donpunha W, et al. Tetrahydrocurcumin alleviates hypertension, aortic stiffening and oxidative stress in rats with nitric oxide deficiency. Hypertens Res 2012; 35: 418-25.
16. Katakam PV, Ujhelyi MR, Hoenig ME, Miller AW. Endothelial dysfunction precedes hypertension in diet-induced insulin resistance. Am J Physiol 1998; 275: R788-92.
17. Ajay M, Chai HJ, Mustafa AM, Gilani AH, Mustafa MR. Mechanisms of the anti-hypertensive effect of Hibiscus sabdariffa L. calyces. J Ethnopharmacol 2007; 109: 388-93.
18. Palmer RM, Ashton DS, Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature 1988; 333: 664-6.
19. Glushakova O, Kosugi T, Roncal C, Mu W, Heinig M, Cirillo P, et al. Fructose induces the inflammatory molecule ICAM-1 in endothelial cells. J Am Soc Nephrol 2008; 19: 1712-20.
20. Potenza MA, Addabbo F, Montagnani M. Vascular actions of insulin with implications for endothelial dysfunction. Am J Physiol Endocrinol Metab 2009; 297: E568-77.
21. Hininger-Favier I, Benaraba R, Coves S, Anderson RA, Roussel AM. Green tea extract decreases oxidative stress and improves insulin sensitivity in an animal model of insulin resistance, the fructose-fed rat. J Am Coll Nutr 2009; 28: 355-61.
22. Farombi EO, Fakoya A. Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers of Hibiscus sabdariffa L. Mol Nutr Food Res 2005; 49: 1120-8.
23. Tseng TH, Kao ES, Chu CY, Chou FP, Lin Wu HW, Wang CJ. Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes. Food Chem Toxicol 1997; 35: 1159-64.
Downloads
Published
2018-06-22
How to Cite
1.
Bunbupha S, Prachaney P, Berkban T, Kukongviriyapan U, Pakdeechote P. Hibiscus sabdariffa L. Extract Alleviates Vascular Endothelial Dysfunction by Enhancing Nitric Oxide Bioavailability in High-Fructose Diet Induced Insulin Resistance Rats. SRIMEDJ [Internet]. 2018 Jun. 22 [cited 2024 Apr. 26];33(4):294-300. Available from: https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/130005
Issue
Section
Original Articles
