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Background and Objective : Bioactive food-derived peptides possess the ability to promote wellness or reduce the risk of diseases. The natural antioxidants have been proposed as alternative therapeutic agents for Alzheimer’s disease. It also has been reported that pigmented rice (Oryza sativa L.) is a natural antioxidants. Therefore, The present study aimed to determine the neuroprotective effect of protein hydrolysate type A-2 from pigmented rice against b-amyloid (Ab) injected rats.
Methods : Male adult Wistar rats were orally given aqueous protein hydrolysate type A-2 extract of pigmented rice (HPR) at various doses ranging from 10, 20, and 40 mg/kg BW for 9 weeks. At day 56, Ab25-35 was injected via both sides of lateral ventricles. After 10 days of Aβ injection, rats were tested for cognitive performance using Novel object recognition tasks.
Results : Aβ25-35 obviously exhibited cognitive deficits by decreasing the discriminative index. When compare to the V plus Ab group, HPR20-, HPR40-treated rats showed a significantly higher discriminative index during 5 minutes and 24 hours delay testing phase, reflecting the increase of learning, short-term and long-term recognition memory of HPR type A-2.
Conclusions : These findings do provide initial evidence that HPR type A-2 may be benefit to be used for
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