The Effect of Chrysin on Memory Impairments in Aging Rats Induced by D-galactose

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Ram Prajit Nataya Sritawan Kornrawee Suwannakot Salinee Naewla Anusara Aranarochana Apiwat Sirichoat Wanassanan Pannangrong Jariya Umka Welbat


Background and Objective:         Brain aging is one of aging problems that affects to the neural stem cells in the subgranular zone (SGZ) of the dentate gyrus (DG) in hippocampus, which exhibits cognitive impairments. D-galactose (D-gal) could induces neuronal apoptosis caused by oxidative stress and inflammation. It is used in several brain aging studies. Chrysin, one of flavonoid, has many neuroprotective effects that can improve memory. This study investigated the effects of chrysin on memory impairments in aging rats induced by D-gal.

Methods:             Male Sprague Dawley rats were divided into 6 groups; control, D-gal, chrysin 10, chrysin 30, D-gal + chrysin 10 and D-gal + chrysin 30 groups. D-gal (50 mg/kg) was administrated by intraperitoneal (i.p.) injection. Chrysin (10 and 30 mg/kg) was administrated by oral gavage. Both of D-gal and chrysin were administrated for 8 weeks. After treatments, the body weight and locomotor activity were determined. The memories were evaluated using novel object location (NOL) and novel object recognition (NOR) tests.

Results:                The results showed that the body weight and locomotor activity did not significant differences among all groups. In the memory tests, control, chrysin 10, chrysin 30, D-gal + chrysin 10 and D-gal + chrysin 30 groups showed significant differences to discriminate the novel location and object in the NOL and NOR tests. In contrast, D-gal group showed no significant difference of discrimination in the both tests.

Conclusion:        This study demonstrates that D-gal induced memory impairments. However, chrysin could attenuated the memory impairments caused by D-gal.


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1. Kitabatake Y, Sailor KA, Ming GL, Song H. Adult neurogenesis and hippocampal memory function: new cells, more plasticity, new memories? Neurosurg Clin N Am 2007; 18: 105-13.
2. Wu B, Chen Y, Huang J, Ning Y, Bian Q, Shan Y, et al. Icariin improves cognitive deficits and activates quiescent neural stem cells in aging rats. J Ethnopharmacol 2012; 142: 746-53.
3. Lazarov O, Mattson MP, Peterson DA, Pimplikar SW, van Praag H. When neurogenesis encounters aging and disease. Trends Neurosci 2010; 33: 569-79.
4. Pourmemar E, Majdi A, Haramshahi M, Talebi M, Karimi P, Sadigh-Eteghad S. Intranasal Cerebrolysin Attenuates Learning and Memory Impairments in D-galactose-Induced Senescence in Mice. Exp Gerontol 2017; 87: 16-22.
5. Yoo DY, Kim W, Lee CH, Shin BN, Nam SM, Choi JH, et al. Melatonin improves D-galactose-induced aging effects on behavior, neurogenesis, and lipid peroxidation in the mouse dentate gyrus via increasing pCREB expression. J Pineal Res 2012; 52: 21-8.
6. Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, et al. Ginsenoside Rg1 prevents cognitive impairment and hippocampus senescence in a rat model of D-galactose-induced aging. PLoS One 2014; 9: e101291.
7. Shwe T, Pratchayasakul W, Chattipakorn N, Chattipakorn SC. Role of D-galactose-induced brain aging and its potential used for therapeutic interventions. Exp Gerontol 2018; 101: 13-36.
8. Hsieh HM, Wu WM, Hu ML. Soy isoflavones attenuate oxidative stress and improve parameters related to aging and Alzheimer's disease in C57BL/6J mice treated with D-galactose. Food Chem Toxicol 2009; 47: 625-32.
9. Ansari NA, Dash D. Amadori glycated proteins: role in production of autoantibodies in diabetes mellitus and effect of inhibitors on non-enzymatic glycation. Aging Dis 2013; 4: 50-6.
10. Nabavi SF, Braidy N, Habtemariam S, Orhan IE, Daglia M, Manayi A, et al. Neuroprotective effects of chrysin: From chemistry to medicine. Neurochem Int 2015; 90: 224-31.
11. Yao Y, Chen L, Xiao J, Wang C, Jiang W, Zhang R, et al. Chrysin Protects against Focal Cerebral Ischemia/Reperfusion Injury in Mice through Attenuation of Oxidative Stress and Inflammation. Int J Mol Sci 2014; 15: 20913–926.
12. Filho CB, Jesse CR, Donato F, Giacomeli R, Del Fabbro L, da Silva Antunes M, et al. Chronic unpredictable mild stress decreases BDNF and NGF levels and Na(+),K(+)-ATPase activity in the hippocampus and prefrontal cortex of mice: antidepressant effect of chrysin. Neuroscience 2015; 289: 367-80.
13. Kandhare AD, Shivakumar V, Rajmane A, Ghosh P, Bodhankar SL. Evaluation of the neuroprotective effect of chrysin via modulation of endogenous biomarkers in a rat model of spinal cord injury. J Nat Med 2014; 68: 586-603.
14. He XL, Wang YH, Bi MG, Du GH. Chrysin improves cognitive deficits and brain damage induced by chronic cerebral hypoperfusion in rats. Eur J Pharmacol 2012; 680: 41-8.
15. Souza LC, Antunes MS, Filho CB, Del Fabbro L, de Gomes MG, Goes AT, et al. Flavonoid Chrysin prevents age-related cognitive decline via attenuation of oxidative stress and modulation of BDNF levels in aged mouse brain. Pharmacol Biochem Behav 2015; 134: 22-30.
16. Dix SL, Aggleton JP. Extending the spontaneous preference test of recognition: evidence of object-location and object-context recognition. Behav Brain Res 1999; 99: 191-200.
17. Umka J, Mustafa S, ElBeltagy M, Thorpe A, Latif L, Bennett G, et al. Valproic acid reduces spatial working memory and cell proliferation in the hippocampus. Neuroscience 2010; 166: 15-22.
18. Kessels RP, de Haan EH, Kappelle LJ, Postma A. Varieties of human spatial memory: a meta-analysis on the effects of hippocampal lesions. Brain Res Brain Res Rev 2001; 35: 295-303.
19. Sirichoat A, Chaijaroonkhanarak W, Prachaney P, Pannangrong W, Leksomboon R, Chaichun A, et al. Effects of Asiatic Acid on Spatial Working Memory and Cell Proliferation in the Adult Rat Hippocampus. Nutrients 2015; 7: 8413-23.
20. Chaisawang P, Sirichoat A, Chaijaroonkhanarak W, Pannangrong W, Sripanidkulchai B, Wigmore P, et al. Asiatic acid protects against cognitive deficits and reductions in cell proliferation and survival in the rat hippocampus caused by 5-fluorouracil chemotherapy. PLoS One 2017; 12: e0180650.
21. Clarke JR, Cammarota M, Gruart A, Izquierdo I, Delgado-Garcíab JM. Plastic modifications induced by object recognition memory processing. Proc Natl Acad Sci U S A. 2010; 107: 2652–57.
22. Fardell JE, Vardy J, Shah JD, Johnston IN. Cognitive impairments caused by oxaliplatin and 5-fluorouracil chemotherapy are ameliorated by physical activity. Psychopharmacology (Berl) 2012; 220: 183-93.
23. Li JJ, Zhu Q, Lu YP, Zhao P, Feng ZB, Qian ZM, et al. Ligustilide prevents cognitive impairment and attenuates neurotoxicity in D-galactose induced aging mice brain. Brain Res 2015; 1595: 19-28.