Four Main Components of Phikud Navakot Promote Cholesterol Metabolism Through LDLR, HMGCR, SR-BI and ApoA-I Genes

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Napatara Tirawanchai Sudarat Supapornhemin Anchaleekorn Somkasetrin Jiraporn Jantaravinid Kanchana Kengkoom Sumate Ampawong

Abstract

Background and Objectives: Ya-Hom Navakot (NY), a combination of fifty-four Thai medicinal herbs, has been used as a traditional medicine for decades especially when dizziness and fainting. Phikud Navakot (PN), nine selected herbal remedies from those components in NY, regulates HMGCR and LDLR genes leading to enhance cholesterol homeostasis. The cholesterol-lowering effect of PN is found to be more potent than that observed in NY. Hence, the objective of this study was to assess the cholesterol-lowering effect of all nine individual herbal extracts of PN which might be used as an alternative treatment for hypercholesterolemia.


 


Methods: Lipid lowering effect of the ethanolic extract of all nine individual herbal extracts of PN was examined focusing on expression of the genes encoding LDLR, HMGCR, SRBI and ApoAI in HepG2 cells by quantitative real-time PCR (qRT-PCR).


Results: The ethanolic extracts from all nine individual herbs of PN were found to downregulate expression of the HMGCR gene comparing with the effect of simvastatin. The extracts of Kot Soa (Angelica dahurica; AD), Kot Khamao (Atractylodes lancea; AL), and Kot Jatamansri (Nardostachys jatamansi; NJ) could additionally upregulate the LDLR and SRB1 genes. Kot Chulalumpa (Artemisia pallens; AP) increased the expression of the ApoA1 gene.


Conclusions: Cholesterol-lowering effect of PN was attributable to the four ingredients of PN which possessed high capability to decrease cholesterol production, maintain cholesterol balance, and promote cholesterol clearance via regulation of the HMGCR, LDLR, and ApoAI genes, respectively. Hence, PN might be an alternative tool to reduce cholesterol level in the future. 

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