Photoauto-, Photohetero- and Photomixotrophic in vitro Propagation of Papaya (Carica papaya L.) and Response of Seed and Seedlings to Light-emitting Diodes

Main Article Content

Jaime A. Teixeira da Silva

Abstract

The objective of this study was to assess whether papaya could be propagated under photoautotrophic conditions and what the response would be to light-emitting diodes (LEDs). Seeds of papaya (Carica papaya L. cv. ‘Rainbow’ and ‘Sunrise Solo’) that were soaked overnight and surface sterilized with 0.1% mercuric chloride solution + 2 drops of Tween-20 then with 80% ethanol, could germinate at 100% on Murashige and Skoog medium with 3% sucrose. Seeds were placed under different light conditions: positive control (100% white heat fluorescent lamps), negative control (darkness) and five combinations of LEDs with different red (R) and blue (B) ratios (100% R; 70% R + 30% B; 50% R + 50% B; 30% R + 70% B; 100% B) with a standard light intensity of 45 μmol/m2/s for all treatments. Seed germination was high (95%-100%) independent of the treatment, but 100% R resulted in extremely long hypocotyls while and 100% B showed highly stunted seedlings. Control seedlings germinated in light were also placed under the same light conditions and stem and root growth were stimulated or stunted by R and B LEDs, respectively. Separately, one-month-old papaya plantlets were cultured photoautotrophically, i.e., in sucrose-free medium in the presence of 3000 ppm CO2, either in non-aerated or aerated vessels. Even though leaf-drop was high, photoautotrophic in vitro propagation led to greater leaf production than control plantlets. Papaya could be propagated under photoautotrophic conditions and LEDs affected seedling growth differently.

Keywords

Article Details

How to Cite
Teixeira da Silva, J. A. (1). Photoauto-, Photohetero- and Photomixotrophic in vitro Propagation of Papaya (Carica papaya L.) and Response of Seed and Seedlings to Light-emitting Diodes. Science & Technology Asia, 19(1), 57-71. Retrieved from https://www.tci-thaijo.org/index.php/SciTechAsia/article/view/17078
Section
Articles
Author Biography

Jaime A. Teixeira da Silva

Faculty of Agriculture and Graduate School of Agriculture, Kagawa University,Miki cho, Kita Gun, Ikenobe, 761-0795, Japan