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The studies of ant diversity in a mango orchard in Phitsanulok province during June to August 2017 was investigated. Ants were collected by using two random sampling methods; manual catching and syrup trapping. The result revealed that 5 subfamily, 21 genera and 28 species of ants. were obtained in this study. Five subfamilies obtained are comprised of Dolichoderinae, Formicinae, Myrmicinae, Ponerinae and Pseudomyrmecinae. The most common species of ant with wide distribution in the study area were Anoplolepis gracilipes (F. Smith, 1857) and Diacamma vargen (F. Smith, 1860). The analysis of Shannon – Wiener’s diversity index and dominance index showed diversity of ant (J'= 0.233) and there was no dominant species of ant in the study area (C = 0.235).