SCCmec types of methicillin-resistant Staphylococcus aureus isolated in 2010, 2015 and 2016 from patients of Srinagarind Hospital, Khon Kaen Province

Abstract

Methiciliin-resistant Staphylococcus aureus (MRSA) has been a major cause of nosocomial infection worldwide. Recently, community-acquired MRSA infections have been reported increasingly. Nowadays, 12 types of Staphylococcal Chromosome Cassette mec (SCCmec) elements have been identified by using multiplex PCR methods. In Thailand, SCCmec type III and type IIIA were mostly reported. Ten years later, various SCCmec types of MRSA had been reported. MRSA isolates in different area may have different evolution and strains variation. The objective of this study was to determine the SCCmec types of MRSA isolates from patients in Srinagarind Hospital in 2010, 2015 and 2016 by using multiplex PCR technique. The SCCmec types were specified by combination of mec gene complex class and ccr complex type. Of the 19 MRSA isolates in 2010, 11 (57.9%) isolates were SCCmec type III, 7 (36.8%) were SCCmec type II, and 1 (5.3%) isolate was undetermined for the SCCmec type. Of the 58 MRSA isolated between 2015-2016, 27 (46.5%) isolates were SCCmec type II, 14 (24.1%) were SCCmec type III, 14 (24.1%) were SCCmec type type I, 1 (1.7%) was type IV, 1 (1.7%) was type IX and 1 (1.7%) isolate was undetermined for the SCCmec type. In addition, no pvl gene was found in the type IV MRSA isolate. This study reported MRSA isolates in 2010 has the trend of SCCmec types like the previous study in 2007 and the results of MRSA isolated between 2015 to 2016 reported the trend of changing in the SCCmec types of the MRSA isolates in this area.