Inhibitory Effect of Essential Oils on the Growth of Aspergillus flavus

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Dusanee Thanaboripat* Yaowapa Suvathi Prapaporn Srilohasin Saowalak Sripakdee Oraphan Patthanawanitchai Sittichai Charoensettasilp

Abstract

The effects of 16 essential oils from aromatic plants were tested for their inhibitory effect on Aspergillus flavus IMI 242684 on PDA. The results showed that the essential oil of white wood (Melaleuca cajeputi) gave the highest inhibition followed by the essential oils of cinnamon (Cinnamomum cassia) and lavender (Lavandula officinalis), respectively. Furthermore, the inhibitory effects of these three essential oils at different concentrations were examined. It was found that the essential oil of white wood at 1.5628% (v/v) and of cinnamon and lavender at 50% (v/v) were the optimum concentrations for fungal growth inhibition. The essential oil of white wood at 25% (v/v) completely inhibited the growth of A. flavus IMI 242684 on PDA for 28 days.


Keywords: essential oils, aromatic plants, aflatoxin, Aspergillus flavus


Corresponding author: E-mail:  ktdusane@kmitl.ac.th

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References

[1] Kurtzman, C. P., Horn, B. W. and Hesseltine, C. W. 1987 Aspergillus nomius, a new aflatoxin producing species related to Aspergillus tamari, Antonie van Leeuwenhoek, 53, 147-158.
[2] Goto, T., Ito, Y., Peterson, S.W. and Wicklow, D.T. 1997 Mycotoxin production ability of Aspergillus tamari, Mycotoxins, 44, 17-20.
[3] Peterson, S.W., Ito, Y., Horn, B.W. and Goto, T. 2001 Aspergillus bombycis, a new aflatoxigenic species and genetic variation in its sibling species, A. nomius, Mycologia, 93(4), 689-703.
[4] Dvorackova, I. 1990 Aspergillus and Human Health. CRC Press, Inc., Boca Raton, Fla.
[5] Smith, J.E. and Moss, M.O. 1985 Mycotoxins. Formation, Analysis and Significance. Chichester., John Wiley & Son.
[6] Smith, J.E. 2004 Biotechnology. 4th ed. Cambridge University Press, Cambridge.
[7] Reddy, D.V.R., Thirumala-Devi, K., Reddy, S.V., Waliyar, F., Mayo, M.A., Rama Devi, K., Ortiz, R. and Lenne, J.M. 2002 Estimation of aflatoxin levels in selected foods and feeds in India. In: Hanak, E., Boutrif, E, Fabre, P. and Pineiro, M., Eds. Food Safety Management in Developing Countries. Proceedings of the International Workshop, CIRAD-FAO, Montpellier, France, pp. 1-4.
[8] Sripathomswat, N. and Thasnakorn, P. 1981 Survey of aflatoxin-producing fungi in certain fermented foods and beverages in Thailand, Mycopathologia., 73, 83-88.
[9] Roy, A.K., Sinha, K.K. and Chourasia, H.K. 1988 Aflatoxin contamination of some common drugs, Applied and Environmental Microbiology, 54(3), 842-843.
[10] Aziz, N.H., Youssef, Y.A., El-Fouly, M.Z. and Moussa, L.A. 1998 Contamination of some common medicinal plant samples and spice by fungi and their mycotoxins, Botanica Bulletin Academica Sinica, 39, 279-285.
[11] Yin, Mei-Chin and Cheng, Wen-Shen. 1998 Inhibition of Aspergillus niger and Aspergillus flavus by some herbs and spices, Journal of Food Protection, 61(1),123-125.
[12] Mahmoud, A.-L.E. 1999 Inhibition of growth and aflatoxin biosynthesis of Aspergillus flavus by extracts of some Egyptian plants, Letters in Applied Microbiology, 29, 334-336.
[13] Thanaboripat, D. 2002 Importance of aflatoxins, KMITL Science Journal, 2(1), 38-45.
[14] Thanaboripat, D. 2003 Mycotoxins: Occurrence and control in foods. In: The International Review of Food Science and technology. IUFoST, November, 2003, pp. 131-133.
[15] Masood, A., Dogra, J.V.V. and Jha, A.K. 1994 The influence of colouring and pungent agents of red chili (Capsicum annum) on growth and aflatoxin production by Aspergillus flavus, Letters in Applied Microbiology, 18, 184-186.
[16] Prasad, G., Sahay, S.S. and Masood, A. 1994 Inhbition in aflatoxin biosynthesis by the extract of Amorphophallus campanulatus (OL) and calcium oxalate, Letters in Applied Microbiology, 18, 203-205.
[17] Thanaboripat, D., Naranong, N. and Peerapakorn, N. 1989 Effect of some herbs on growth of Aspergillus flavus and aflatoxin production, Srinakarinwirot Journal of Science, 5, 33-39.
[18] Thanaboripat, D., Nontabenjawan, K., Leesin, K., Teerapiannont, D., Sukcharoen, O. and Ruangrattanametee, V. 1997 Inhibitory effect of garlic, clove and carrot on growth of Aspergillus flavus and aflatoxin production, Journal of Forestry Research, 8, 39-42.
[19] Thanaboripat, D., Cheunoy, W., Petcharat, U., Ruangrattanametee, V. and Krisisntu, K. 2000 Control of aflatoxigenic fungi by Thai neem, Government Pharmaceutical Organization Journal, 21, 41-49.
[20] Thanaboripat, D., Monkontanawut, N., Suvathi, Y. and Ruangrattanametee, V. 2004 Inhibition of aflatoxin production and growth of Aspergillus flavus by citronella oil, KMITL Science Journal, 49(1), 1-8.
[21] Thanaboripat, D., Suvathi, Y. and Chantarateptawan, V. 2005 Control of growth and aflatoxin production of Aspergillus flavus by medicinal and aromatic plants. In : Study on Plant Pest and Disease Biological Control and Bio-technology, pp.52-62. Edited by Yang Qian and Yu Ziniu, Heilongjiang Science and Technology Press, Harbin.
[22] Bankole, S. A. and Joda, A. O. 2004 Effect of lemon grass (Cymbopogon citrate Stapf) powder and essential oil on mould deterioration and aflatoxin contamination of melon seeds (Colocynthis citrullus L.). African Journal of Biotechnology, 3(1), 52-59.
[23] Nunthavutthikul, S. 2000 Studies of microbial inhibition of herbal extracts. Master’s thesis, Faculty of Phamacy Chulalongkorn University, Bangkok.
[24] Lorian, V. 1986 Antibiotics in Laboratory Medicine. Lippincott Williams & Wilkins, Philadelphia.
[25] Charoensettasilp, S. 1999 Experimental Design. Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang., Bangkok.
[26] Brophy, J.J., Thubthimthed, S., Kitirattrakarn, T. and Anantachoke, C. 2002 Volatile leaf oil of Melaleuca cajuputi. In Proceedings of the Forestry Conference, pp. 304-131.
[27] Mahmoud, A.-L.E. 1994 Antifungal action and antiaflatoxigenic properties of some essential oil constituents. Letters in Applied Microbiology, 19, 110-113.
[28] Ozcan, M. 2005 Effect of spice hydrosols on the growth of Aspergillus parasiticus NRRL 2999 strain. Journal of Medicine Food, 8(2), 275-278.
[29] Montes-Belmont, R. and Carvajal, M. 1998 Control of Aspergillus flavus in maize with plant essential oils and their components. Journal of Food Protection, 61, 616-619.
[30] Zaika, L.L. and Buchanan, R.L. 1987 Review of compounds affecting the biosynthesis or bioregulation of aflatoxins. Journal of Food Protection, 50(8), 691-708.
[31] Sinha, K.K., Sinha, A.K. and Prasad, G. 1993 The effect of clove and cinnamon oils on growth of and aflatoxin production by Aspergillus flavus. Letters in Applied Microbiology, 16, 114-117.
[32] Conner, D. E. 1993 Naturally occurring compounds, In Antimicrobial in Foods, pp. 441-468. Edited by Davidson, P. M. and Branen, A.L. Marcl Dekker Inc., New York.
[33] Snyder, O.P. 1997 Antimicrobial Effects of Spices and Herbs. Hospitality Institute of Technology and Management, St Paul, Minnisota.
[34] Charles, D. J. and Simon, J.E. 1990 Comparison of extraction methods for the rapid determination of essential oil content and composition of basil. Journal of American Horticultuel Science, 115, 485-462.
[35] Paakkonen, K., Malmsten, T. And Hyvonen, L. 1990 Drying, packaging and storage effects on quality of basil, marjoram and wild marjoram. Journal of Food Science, 55, 1373-1382.
[36] Moretti, M. D. L., Sanna-Passino, G., Demontis, S. and Bazzoni, E. 2002 Essential oil formulations useful as a new tool for insect pest control. AAPS PharmSciTech. 3(2), 1-11.