Determination of Hepatitis B Surface Antigen Particles by Elisa

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U.N. Ekwenye*


Hepatitis B surface Antigen (HBsAg) expressed in Pichia pastoris assemble into particulate structures. Enzyme-linked immunosorbent assay (ELISA) was used to determine these particles. The covalent attachment of enzymes to antibody molecules created an immunological tool possessing both high specificity and high sensitivity. The specificity, rapidity and inexpensive nature of ELISAs have insured their prominence in clinical and veterinary medicine.

Keywords: Hepatits B, Pichia pastoris, ELISA, clinical and veterinary medicine, Antigen

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