Consolidated Bioprocessing of Ethanol from Corn Straw by Saccharomyces Diastaticus and Wikerhamomyces Chambardii
DOI:
https://doi.org/10.14456/fabj.2018.1Keywords:
Consolidated bioprocessing, Cellulosic ethanol, Yeast, W. chambardii, S. diastaticusAbstract
Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one-step process, is a promising strategy for cost-effective ethanol production from starchy biomass. Two yeast strains namely; Saccharomyces diastaticus and Wickerhamomyces chambardii were selected for direct bioethanol production from corn straw medium for 72 h. These strains were able to utilize cellulose directly and highest bioethanol production was recorded at 48 h of fermentation. Maximum ethanol production was achieved by S. diastaticus at 35 °C, pH 5.0 in the medium containing 7.5% (w/v) during fermentation. The yeast isolates were able to tolerate wide ranges of temperature, pH and substrate concentration for higher ethanol production. This study presents the potential of S. diastaticus and W. chambardii in cellulose-based ethanol production by consolidated bioprocessing. This strategy will eliminate multistage process of ethanol production which will lead to reduction of the overall production cost.
References
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Shafei, M.S. and Allam R.F. 2010. Production and immobilization of partially purified lipase from Penicillium chrysogenum. Malaysian Journal of Microbiology. 6(2): 196–202.
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Sláviková, E. and Vadkertiová, R. 2003. The diversity of yeasts in the agricultural soil. Journal of Basic Microbiology. 43(5): 430–436.
Sripiromrak, A. 2006. Isolation and characterization of thermotolerant yeast for ethanol production.Master Thesis, Suranaree University of Technology. 1–98.
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Wakil, S.M., Adelabu, A.B., Fasiku, S.A. and Onilude, A.A. 2013. Production of bioethanol from palm oil mill effluent using starter cultures. New York Science Journal. 6(3): 77–85.
Amutha, R. and Gunasekaran, P. 2000. Improved ethanol production by a mixed culture of Saccharomyces diastaticus and Zymomonas mobilis from liquefied cassava starch. Indian Journal of Microbiology. 40: 103–107.
Aransiola, E.F., Betiku, E. Adetunji, O.A. and Solomon, B.O. 2006. Production of baker's yeast (Saccharomyces cerevisae) from raw cassava starch hydrolyzates in a bioreactor under batch process. Biotechnology Journal. 5(1): 98–103.
Ariyajaroenwong, P., Laopaiboon, P., and Laopaiboon, L. 2012. Repeated-batch ethanol production from sweet sorghum juice by Saccharomyces cerevisiae immobilized on sweet sorghum stalks. Energies. 5: 1215–1228.
Balat, M., Balat, H. and Öz, C. 2008. Progress in bioethanol processing. Progress in Energy and Combustion Science. 34(5): 551–573.
Barnett, J.A. 2003. A history of research on yeasts 6: the main respiratory pathway. Yeast. 20(12): 1015–1044.
Bettiga, M., Bengtsson, O., Hahn-Hägerdal, B. and Gorwa-Grauslund, M.F. 2009. Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway. Microbial Cell Factories. 8(40): 1–12.
Bodade, R.G., Khobragade, C.N. and Arfeen, S. 2010. Optimization of culture conditions for glucose oxidase production by a Penicillium chrysogenum SRT 19 strain. Engineering in Life Sciences. 10(1): 35–39.
Brooks, A.A. 2008. Ethanol production potential of local yeast strains isolated from ripe banana peels. African Journal of Biotechnology. 7(20): 3749–3752.
Butinar, L., Santos, S., Spencer-Martins, I., Oren, A. and Gunde-Cimeraman, N. 2005. Yeast diversity in hypersaline habitats. FEMS Microbiology Letters. 244: 229–234.
Chanchaichaovivat, A., Ruenwongsa, P. and Panijpan, B. 2007. Screening and identification of yeast strains from fruits and vegetables: Potential for biological control of postharvest chilli anthracnose (Colletotrichum capsici). Biological Control. 42: 326–335.
Couto. S.R., and Sanromán, M.A. 2006. Application of solid-state fermentation to food industry—A review. Journal of Food Engineering. 76(3): 291–302.
García, V.De., Brizzio, S. Libkind, D. Rosa, C.A. and Broock, M.V. 2010. Wickerhamomyces patagonicus sp. nov., an ascomycetous yeast species from Patagonia, Argentina. International Journal of Systematic and Evolutionary Microbiology. 60: 1693–1696.
Hashem, M., Zohri, A.N.A. and Maysa, M.A.A. 2013. Optimization of the fermentation conditions for ethanol production by new thermotolerant yeast strains of Kluyveromyces sp. African Journal of Microbiplogy Research. 7(37): 4550–4561.
Iqbal, H.M.N., Asgher, M., Ahmed, I. and Hussain, S. 2010. Media optimization for hyper-productionof carboxymethyl cellulase using proximally analysed agro-industrial residues with Trichoderma harzianum under SSF. International Journal for Agro Veterinary and Medical Sciences. 4(2): 47–55.
Kareem, S.O., Akpan, I. and Oduntan, S.B. 2009. Cowpea waste: A novel substrate for solid state production of amylase by Aspergillus oryzae. African Journal of Microbiological Research. 3(12): 974–977.
Khan, Z. and Dwivedi. A.K. 2013. Fermentation of biomass for production of ethanol. Universal Journal of Environmental Research and Technology. 3(1): 1–13.
Kongkiattikajorn, J. and Sornvoraweat, B. 2011. Comparative study of bioethanol production from cassava peels by monoculture and co-culture of yeast. Kasetsart Journal of Natural Science. 45(2): 268–274.
Kurian, J.K., Minu, K.A., Banerjee, A. and Kishore, V.V.N. 2010. Bioconversion of hemicellulose hidrolysate of sweet sorghum bagasse to ethanol by using Pichiastipitis NCIM 3497 and Debaryomyces hansenii sp. Bioresources. 5(4): 2404–2416.
Kurtzman, C.P., 2011. Wickerhamomyces Kurtzman, Robnett & Basehoar-Powers (2008). The Yeasts, A Taxonomic Study. 5th edn. 899–917.
Lazarova, G., Kostov, V. and Sokolov, T. 1987. A new method for ethanol productivity determination. Biotechnology Techniques. 1(2): 123–126.
Limtong, S., Sringiew, C., Yongmanitchai, W. 2007. Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marixianus. Bioresource Technology. 98(17): 3367–3374.
Lin, Y., Zhang, W., Li, C., Sakakibara, K., Tanaka, S. and Kong, H. 2012. Factors affecting ethanol fermentation using Saccharomyces cerevisiae BY4742. Biomass and Bioenergy. 47: 395–401.
Mazmanci, M.A. 2011. Ethanol production from Washingtonia robusta fruits by using commercial yeast. African Journal of Biotechnology. 10(1): 48–53.
Murado, M.A., Pastrana, L., Vázquez, J.A., Mirón, J. and González, M.P. 2008. Alcoholic chestnut fermentation in mixed culture. Compatibility criteria between Aspergillus oryzae and Saccharomyces cerevisiae strains. Bioresource Technology. 99(15): 7255–7263.
Osho, A. 2005. Ethanol and sugar tolerance of wine yeasts isolated from fermenting cashew apple juice. African Journal of Biotechnology. 4(7): 660–662.
Oyeleke, S.B. and Jibrin, N.M. 2009. Production of bioethanol from guinea cornhusk and millet husk. African Journal of Microbiology Research. 3(4): 147–152.
Rai, P., Tiwari, S. and Guar, R. 2012. Optimization of process parameters for cellulase production by novel thermotolerant yeast. Bioresources. 7(4): 5401–5414.
Saliu, B.K. 2012. Production of ethanol from some cellulosic waste biomass hydrolyzed using fungal cellulases. Doctor of Philosophy Thesis, University of Ilorin. 1–160.
Saravanakumar, K., Senthilraja, P. and Kathiresan, K. 2013. Bioethanol production by mangrove-derived marine yeast Sacchromyces cerevisiae. Journal of King Saud University – Science. 25(2): 121–127.
Sarris, D. and Papanikolaou, S. 2016. Biotechnological production of ethanol. Biochemistry, processes and technologies. Engineering in life sciences. 16(4): 307–329.
Shafei, M.S. and Allam R.F. 2010. Production and immobilization of partially purified lipase from Penicillium chrysogenum. Malaysian Journal of Microbiology. 6(2): 196–202.
Shah, RK., Shah, RK. and Madamwar, D. 2006. Improvement of the quality of whole wheat bread by supplementation of xylanase from Aspergillus foetidus. Bioresources Technology. 97(16): 2047–2053.
Sláviková, E. and Vadkertiová, R. 2003. The diversity of yeasts in the agricultural soil. Journal of Basic Microbiology. 43(5): 430–436.
Sripiromrak, A. 2006. Isolation and characterization of thermotolerant yeast for ethanol production.Master Thesis, Suranaree University of Technology. 1–98.
Valcheva, I., Yavorov, N. and Petrin, S. 2016. Topchemical kinetics mechanism of cellulose hydrolysis on fast-growing tree species. Cost Action FP1105. Holzforschung. 70(12): 1147–1153.
Wakil, S.M., Adelabu, A.B., Fasiku, S.A. and Onilude, A.A. 2013. Production of bioethanol from palm oil mill effluent using starter cultures. New York Science Journal. 6(3): 77–85.
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Published
2017-07-06
How to Cite
Adelabu, Blessing, Sarafadeen Kareem, Flora Oluwafemi, and Abideen Adeogun. 2017. “Consolidated Bioprocessing of Ethanol from Corn Straw by Saccharomyces Diastaticus and Wikerhamomyces Chambardii”. Food and Applied Bioscience Journal 6 (1):1-17. https://doi.org/10.14456/fabj.2018.1.
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Food Processing and Engineering
