Differential Phosphoproteomics Analysis to Identify Proteins and Pathways of Human Lung Epithelial Cells During Exposure to Burkholderia Pseudomallei

  • Supannika Namwongnao Student, Master of Science Program in Medical Microbiology, Departments of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Sittiruk Roytrakul National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Devlopment Agency (NSTDA), Thailand
  • Viraphong Lulitanond Professor, Departments of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Kiatichai Faksri Associate Professor, Departments of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Wises Namwat Assistant Professor, Departments of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
Keywords: Burkholderia psudomalliei, Bp1026b, A549, Phosphoprotein, Human lung epithelial

Abstract

Burkholderia psudomalliei (Bp) is the causative agent of melioidosis, an infectious disease of humans and animals.  At present there is no available human vaccine that protects against Bp, and with the current limitations of antibiotic treatment, the development of new preventative and therapeutic interventions is crucial.  This study elucidated human epithelial host cell responses immediatly after exposure to Bp.  Forty-five phosphoproteins of human lung epithelial cells were activated after exposure to Bp. Seven cellular pathway were found to be associated with 8 of those activated phosphoproteins. The results suggested the response of the host cell including cell proliferation, gene expression, differentiation, mitosis, cell survival, energy production and apoptosis. These results suggested the rapid response of the host cell and the possible importance of phosphoproteins in host responses to pathogen. Moreover, those phosphoproteins can be fulfill basic knowledge to insight host response to control Bp infection.

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Published
2019-03-04
Section
บทความวิจัย