Efficacy study of Real-time PCR and standard method for Mycobacterium tuberculosis and its multi drug-resistance diagnosis at Nakhonpathom Hospital
-
Keywords:
Tuberculosis, multi drug-resistant tuberculosis, real-time PCR, standard method, Rifampicin drug (RIF), Isoniazid drug (INH)Abstract
Tuberculosis (TB) is one of the causes of death in the world and is a major problem in Thailand. Culture is a standard method for diagnosis of tuberculosis and multi drug-resistant tuberculosis (MDR-TB) but still have limitations. Currently, the molecular technique is high sensitivity and specificity which is a more popular method for testing. Therefore, the purpose of this study was to evaluate the effectiveness of tuberculosis and multi drug-resistant tuberculosis diagnosis using real-time PCR method compared to the standard method. Sputum specimens from 151 patients evaluating the effectiveness of tuberculosis diagnosis and first line anti-tuberculosis drug resistance such as Rifampicin (RIF) and Isoniazid (INH) testing compared with culture method and drug susceptibility testing. A sensitivity of tuberculosis diagnosis, RIF resistance, and INH resistance using real-time PCR method compared to culture method was 97.26, 100 and 100 respectively. The result of tuberculosis diagnosis, RIF resistance and INH resistance with a specificity was 94.87, 100 and 95.24 respectively. A positive predictive value was 94.67, 100 and 72.73 and a negative predictive value of 97.37, 100 and 100 respectively. The inconsistent results from two methods for the diagnosis of tuberculosis and drug resistance INH found 6 and 3 samples, respectively. This study suggests that the real-time PCR technique is a method that provides accurate, reliable and fast for tuberculosis and its drug-resistant diagnosis for the benefit of diagnosis in patients with TB.
References
2. Kent PT, Kubica GP, Centers for Disease C. Public health mycobacteriology : a guide for the level III laboratory. Atlanta, Ga.: U.S. Dept. of Health and Human Services, Public Health Service, Centers for Disease Control. 1985;1-226
3. Suthum K, Tipkrua N. Comparison of Mycobacterium tuberculosis drug resistance testing between Real-time PCR using a Dual Priming Oligonucleotide Method and drug susceptibility testing by The BACTECTM MGITTM 960 System. J Med Tech Assoc Thailand 2013;41:4654-66.
4. Seegene, Inc., Korea. User Manual: AnyplexTM MTB/NTM Real-time Detection (V2.0). 2015;3.02:15-23.
5. Anatolia Geneworks®, Turkey. User Manual: Magnesia16 Nucleic Acid Extraction Kit. 2012;4.0:17-18.
6. Seegene, Inc., Korea. User Manual: AnyplexTM II MTB/MDR Detection. 2015;1.0:15-28.
7. Kim SW, Kim SI, Lee SJ, Lee JH, Ryu YJ, Shim SS, et.al. The effectiveness of real-time PCR assay, compared with microbiologic results for the diagnosis of pulmonary tuberculosis. Tuberc Respir Dis (Seoul). 2015;78(1):1-7.
8. สมศักดิ์ สินธุอุไร, กัญณภัทร เกษรสุคนธ์, ปัทมา กล่อมพร, ธนกร โพธิ์วงศ์, รัชณีพร คำมินทร์, และสุวรรณี กีรติวาสี. การตรวจวินิจฉัยและการทดสอบความไวต่อยาของเชื้อ วัณโรคด้วยชุดน้ำยาสำเร็จรูป AnyplexTM Real-time PCR เปรียบเทียบกับวิธีมาตรฐานการเพาะเลี้ยงเชื้อ. วารสารเทคนิคการแพทย์เชียงใหม่ ปีที่ 49. 2559;218-226.
Downloads
Published
How to Cite
Issue
Section
License
บทความที่ได้รับการตีพิมพ์ในวารสารหัวหินเวชสาร เป็นลิขสิทธิ์ของโรงพยาบาลหัวหิน
บทความที่ลงพิมพ์ใน วารสารหัวหินเวชสาร ถือว่าเป็นความเห็นส่วนตัวของผู้เขียนคณะบรรณาธิการไม่จำเป็นต้องเห็นด้วย ผู้เขียนต้องรับผิดชอบต่อบทความของตนเอง
