Scavenging Activities and Protective Effects of Syzygium Cumini (L.) Skeels on H2O2 Induce Oxidative Stress in Normal Human Peripheral Blood Mononuclear Cells

Abstract

Syzygium cumini (L.) Skeels is the most commonly used as medicinal plants on diabetes and anti-inflammation. Hence, effects of aqueous leaf extracts of Syzygium cumini (L.) Skeels (ASC) on scavenging activities and protective effects of H₂O₂- induced oxidative damage in human peripheral blood mononuclear cells (PBMC) were determined in this study. Total phenolic and flavonoid contents of ASC at 2,000 µg/ml were 694.44±3.64 µgGAE/ml and 8.27±0.10 µg quercetin/ml respectively which were correlated with the r² value at 0.93. IC₅₀ values of ASC on free radical, hydroxyl and superoxide were 87.79±0.85, 766.62±28.61 and 1,299±25.13 µg/ml respectively while the nitric oxide scavenging activities were not higher than 50% at the concentration of 2,000 µg/ml of ASC. The protective effects of ASC in H₂O₂-induced PBMC cell damages were significantly increased to 83.13±3.57–112.32±4.47 % when treated with 0.25–1 µg/ml of ASC after oxidative induced by 5 mM of H₂O₂ for 3 h. Suggest that ASC is a potential source of natural antioxidant properties and protective defense system of PBMC.