Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation

Main Article Content

Manaphat Suksai Kriengsak Dhanaworavibul

Abstract

Objective: To compare human sperm motility, vitality and morphology after cryopreservation among sucrose, and different trehalose concentrations.
Material and Methods: A total number of 124 normozoospermic semen samples were collected. Each semen sample was divided into 4 portions, and cryopreserved in a human sperm-preserving medium along with cryoprotectants, including; 50 milimolar (mM) trehalose, 100 mM trehalose, 200 mM trehalose and 50 mM sucrose, respectively. All semen samples were frozen by using a vapor phase method. Post-thawed sperm motility, vitality and morphology were assessed. R program was used for data analysis. A p-value of <0.05 was considered statistically significant.
Results: Post-thawed semen evaluation indicated that 50 mM trehalose was better than 50 mM sucrose in all sperm parameters, which included progressive motility (p-value=0.037, total motility (p-value<0.001), vitality (p-value<0.001) and morphology (p-value<0.001). The sperm parameters were not significantly different among 100 mM trehalose, 200 mM trehalose and 50 mM sucrose.
Conclusion: The use of 50 mM trehalose, as non-permeating cryoprotectant, showed superior post-thaw sperm
parameters over sucrose, and other trehalose concentrations.

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How to Cite
1.
Suksai M, Dhanaworavibul K. Effects of Trehalose and Sucrose on Human Sperm Motility, Vitality and Morphology after Cryopreservation. Journal of Health Science and Medical Research [Internet]. 18Mar.2019 [cited 18Oct.2019];37(2):101-7. Available from: https://www.tci-thaijo.org/index.php/jhsmr/article/view/148584
Section
Original Article

References

1. Sadeghi MR. It is time to pay more attention to sperm cryopreservation: now more than ever!. J Reprod Infertil 2016;17:1.

2. Stanic P, Tandara M, Sonicki Z, Simunic V, Radakovic B, Suchanek E. Comparison of protective media and freezing techniques for cryopreservation of human semen. Eur J Obstet Gynecol Reprod Biol 2000;91:65-70.

3. Liu J, Tanrikut C, Wright DL, Lee GY, Toner M, Biggers JD, et al. Cryopreservation of human spermatozoa with minimal non-permeable cryoprotectant. Cryobiology 2016;73:162-7.

4. Shiva Shankar Reddy N, Jagan Mohonarao G, Atreja SK. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim Reprod Sci 2010;119:183-90.

5. Malo C, Gil L, Gonzalez N, Cano R, de Blas I, Espinosa E. Comparing sugar type supplementation for cryopreservation of boar semen in egg yolk based extender. Cryobiology 2010;61:17-21.

6. Thompson KA, Richa J, Liebhaber SA, Storey BT. Dialysis addition of trehalose/glycerol cryoprotectant allows recovery of cryopreserved mouse spermatozoa with satisfactory fertilizing ability as assessed by yield of live young. J Androl 2001;22:339-44.

7. Aboagla EM, Terada T. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod 2003;69:1245-50.

8. Aisen EG, Medina VH, Venturino A. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology 2002;57:1801-8.

9. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: WHO; 2010.

10. Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehninger S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988;49:112-7.

11. Hammadeh ME, Greiner S, Rosenbaum P, Schmidt W. Comparison between human sperm preservation medium and TEST-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze-thawing procedure. J Androl 2001;22:1012-8.

12. Schulz M, Risopatron J, Matus G, Pineda E, Rojas C, Isachenko V, et al. Trehalose sustains a higher post-thaw sperm motility than sucrose in vitrified human sperm. Andrologia 2017;49:1-3.

13. Ozkavukcu S, Erdemli E, Isik A, Oztuna D, Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet 2008;25:403-11.

14. Gao DY, Liu J, Liu C, McGann LE, Watson PF, Kleinhans FW, et al. Prevention of osmotic injury to human spermatozoa during addition and removal of glycerol. Hum Reprod 1995;10:1109-22.

15. Storey BT, Noiles EE, Thompson KA. Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology 1998;37:46-58.

16. Albertorio F, Chapa VA, Chen X, Diaz AJ, Cremer PS. The a,a-(1->1) linkage of trehalose is key to anhydrobiotic preservation. J Am Chem Soc 2007;29:10567-74.

17. Anchordoguy TJ, Rudolph AS, Carpenter JF, Crowe JH. Modes of interaction of cryoprotectants with membrane phospholipids during freezing. Cryobiology 1987;24:324-31.

18. Crowe JH. Anhydrobiosis: an unsolved problem with applications in human welfare. Subcell Biochem 2015;71:263-80.

19. Bastiaan HS, Menkveld R, Oehninger S, Franken DR. Zona pellucida induced acrosome reaction, sperm morphology,
and sperm-zona binding assessments among subfertile men. J Assist Reprod Genet 2002;19:329-34.

20. Vogiatzi P, Chrelias C, Cahill DJ, Creatsa M, Vrachnis N, Iliodromiti Z, et al. Hemizona assay and sperm penetration assay in the prediction of IVF outcome: a systematic review. Biomed Res Int 2013;2013. doi: 10.1155/2013/945825.