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This research aimed to investigate factors affecting the production of polyhydroxyalkanoates (PHAs) from palm oil as a carbon source by Pseudomonas aeruginosa TISTR 1287. The experiments were set-up a batch in an orbital shaker incubator at 30๐C with 180 rpm. Four concentrations (0.50, 0.75, 1.00, 1.50 and 2.00 % (w/v)) of palm oil were tested and initial pH in the culture medium was fixed at 6.90. The results showed that the concentrations of palm oil have the effect on cell growth and cell dry weight of P. aeruginosa TISTR 1287. The maximum cell dry weight at 44 hrs was 2.33 g L-1 obtained from the palm oil concentration of 0.75% (w/v). Moreover, the culture time also affected the cells growth and intracellular accumulation of PHAs. The PHAs concentration and content were increased when increasing the culture time from 0 to 72 hours. The maximum PHAs concentration was 0.65 g L-1 and PHAs content was 38.01 % when cultured in medium with 0.75% (w/v) palm oil at 72 hrs fermentation. The microbial cells in the culture medium showed high red fluorescent, when the cells were determined using the fluorescent dye Nile red. The PHAs granules of intracellular the microbial cell were seen easily (white granules) by transmission electron microscope. The results demonstrated that P. aeruginosa TISTR 1287 can be used palm oil as a carbon source for producing the PHAs based bioplastics in an intracellular.