Virulence of Vibrio parahaemolyticus in Mud Crab (Scylla sp.)
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Published:
Jul 20, 2017
Keywords:
Virulence Mud crab Vibrio parahaemolyticus
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Abstract
The study on virulence of Vibrio parahaemolyticus was challenged by experimental study against mud crab (Scylla sp.). The healthy mud crabs were injected with V. parahaemolyticus at 101-1010 CFU (Colony Forming Unit) per milliliter (ml). After injected for 1 day, the cumulative mortality of crabs was 100% at 108-1010 CFU per ml of bacteria. After injected for 2 days, the cumulative mortality was 100% at 107 CFU per ml of V. parahaemolyticus. During 30 days, the cumulative mortality was 22.22% at 106 CFU per ml of bacteria. Furthermore, during 30 days, the cumulative mortality was 0.00% at 105-101 CFU per ml of V. parahaemolyticus. This study suggesting that the virulence of V. parahaemolyticus causes for rapid mass mortality of mud crab.
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Research Article
References
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Sudheesh, P.S. and Xu, H.S. 2001. Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases. Aquaculture.196: 37-46.
Vogan, C.L. and Rowley, A.F. 2002. Effects of shell disease syndrome on the haemocytes and humoral defences of the edible crab, Cancer pagurus. Aquaculture. 205: 237–252.
Aguirre-Guzmán, G., Sánchez-Martínez, G. J., Perez-Castañeda, R., Palacios-Monzón, A., Trujillo-Rodríguez, T. and Cruz-Hernández, I. N. 2010. Pathogenicity and infection route of Vibrio parahaemolyticus in American White Shrimp, Litopenaeus vannamei. J. World Aquacult. Soc. 41(3): 464-470.
Ayres, P.A. and Edwards, E. 1982. Notes on the distribution of black spot shell disease in crustacean fisheries. Chem. Ecol. 1: 125-130.
Chaweepack, T., Ruangpan, L. and Nuphin, V. 2007. Parasites and vibrio flora of wild broodstock banana shrimp (Penaeus merguiensis) from eastern gulf of Thailand. Technical paper. Coastal Fisheries Research and Development Bureau. Chanthaburi Coastal Fisheries Research and Development Center; Department of Fisheries, Chanthaburi (Thailand). (in Thai)
Costa-Ramos, C. and Rowley, A. F. 2004. Effect of extracellular products of Pseudoalteromonas atlantica on the edible crab Cancer pagurus. Appl. Environ. Microbiol. 70(2): 729-735.
Davis, W. J. and Sizemore, K. R.1982. Incidence of Vibrio species associated with Blue Crabs Callinectes sapidus collected from Galveston Bay, Texas. Appl. Environ. Microbiol. 43(5): 1092-1097.
Direkbusarakom, S. and Rojanapitayakul, S. 1998. Effect of chlorine (Calcium hypochlorite) in seawater and seawater with soil on Vibrio harveyi. Proceedings of the 36th Kasetsart University Annual Conference: Abstracts. (in Thai)
Intanakom, J. 2010. Study on morphological, bacterial identification and histology in mud crab (Scylla sp.) with red thoracic-abdominal syndrome. The thesis of Kasetsart University. Bangkok. (in Thai)
Laoprasert, T. and Laoprasert, S. 2010. Diseases in mud crab, Scylla sp. Thai Fisheries Gazette (Thailand). 63: 265-267. (in Thai)
Laoprasert, T., Laoprasert, S., Soonthornwit, S. and Phonwari, N. 2010. Study on bacterial flora in mud crab (Scylla sp.). Thai Fisheries Gazette (Thailand). 63: 238-241. (in Thai)
Plainpun, N. 2014. Induction of red thoracic – abominal syndrome with bacteria isolated from mud crab (Scylla sp.). The thesis of Kasetsart University. Bangkok. (in Thai)
Promchairat., J., Limsuwan, C. and Chuchird, N. 2013. Experimental exposure and evaluation of Artemia sp. to Vibrio sp. As possible vectors of pathogenic bacteria. Proceedings of 51st Kasetsart University Annual Conference: Veterinary Medicine, Fisheries. (in Thai)
Ruangpan, L., Tanasomwang, W. and Sangrungruang, K. 1997. Bacterial flora of intensively cultured black tiger shrimp (Penaeus monodon). Proceedings of the 35th Kasetsart University Annual Conference: Fisheries, Science, Engineering, Environmental Management, Home Economics, Education and Economics. (in Thai)
Somjetlertcharoen, A. 1993. Study on Vibrio species and oxolinic acid residues from Penaeus monodon Fabricius in intensive culture ponds. The thesis of Kasetsart University. Bangkok. (in Thai)
Srimeetian, P. 2010. Antioxidant and lipid peroxidation in molting cycle of mud crab (Scylla serrata Forskål 1775). The thesis of Kasetsart University. Bangkok. (in Thai)
Srimeetian, P., and Tunkijjanukij, S. 2016. Differential protein expression in haemolymph of first-stage red sternum mud crabs (Scylla serrata). Thai J. Vet. Med. 46(3): 401-407.
Sudheesh, P.S. and Xu, H.S. 2001. Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases. Aquaculture.196: 37-46.
Vogan, C.L. and Rowley, A.F. 2002. Effects of shell disease syndrome on the haemocytes and humoral defences of the edible crab, Cancer pagurus. Aquaculture. 205: 237–252.