Journal of Mahanakorn Veterinary Medicine Faculty of Veterinary Medicine, Mahanakorn University of Technology en-US Journal of Mahanakorn Veterinary Medicine 1905-7571 Geo-Information Techniques for Analysis of Foot and Mouth Disease in Cattle Maeon District, Chiang Mai Province <p>Geo-Information techniques for analysis of Foot and Mouth Disease in cattle in Maeon district, Chiang Mai province has objectives of study: 1) to analyze distribution pattern of foot and mouth disease in cattle with spatial statistics and 2) to analyze relationship of agents influencing foot and mouth disease in cattle. Geo-Information techniques in animal epidemiology and statistical methods were used in this study. Coordinates of cattle farms from GPS were used to analyze point density. This is to derive distribution pattern of foot and mouth disease. Agents are temperature, humidity, and distance from the road. They were extracted from Landsat 8 and find the correlation between distribution of foot and mouth disease with Pearson’s correlation coefficient.&nbsp; The results found that there is significant (P &lt;0.05) correlation between distribution of foot and mouth disease and agents. Humidity is a strongest correlation (r = 0.972, P = 0.000) followed by temperature r = -0.850, P = 0.000) and distance from road (r = -0.486, P = 0.026), respectively.</p> Suchada Onmueang Arisara Charoenpanyanet ##submission.copyrightStatement## 2018-04-24 2018-04-24 13 1 1 15 Species Diversity of Freshwater Snails in Mae Lao Agricultural Basin (Chiang Rai, Thailand) and its Relationship with some Physio-chemical Parameters <p>Mae Lao agricultural basin is located in the Mae Lao sub-river basin of Kok River basin, Chiang Rai province, Thailand. There is a scarcity of information on the diversity of freshwater snails in this area. Herein, the aim of this study was to assessing the freshwater snail diversity and the abundance in relation to the physio-chemical properties in different watercourse. Physio-chemical properties and snails were sampled in 10 sites in Mae Lao district to characterize the snail habitats. Species richness, Shannon’s diversity and evenness indices were employed to describe snail diversity and diversity across the habitat types. Statistical analyses were conducted to examine the extent to which the physio-chemical properties and abundance of snails. Among 3,725 collected snails, 12 snail species namely <em>Bithynia funiculata</em>, <em>B. siamensis goniomphalos</em>, <em>B. s. siamensis</em>, <em>Clea helena</em>, <em>Trochotaia trochoides</em>, <em>Filopaludina doliaris</em>, <em>F. martensi martensi, F. sumatrensis polygramma</em>, <em>Lymnaea auricularia</em>, <em>Melanoides tuberculata</em>, <em>Pomacea canaliculata</em> and <em>Corbicula</em> sp. were identified. 9 out of these 12 snail species are of medical and veterinary importance<em>.</em> <em>B. s. siamensis </em>and <em>M. tuberculata</em> were the most enumeration of snail species. Analysis of the data revealed that the physio-chemical properties no significant difference across the stream and rice paddy habitats. However, freshwater snail abundance was significant correlated with dissolved oxygen and pH in stream habitat (<em>p</em>&lt;0.05). In addition, different habitats had different species diversity and snail abundance with high snail abundance at stream habitats. The present study is the first research on the freshwater snail fauna in Mae Lao agricultural basin and all snail species were recorded for the first time from this area.</p> Kittichai Chantima Chiraya Thesarin ##submission.copyrightStatement## 2018-06-04 2018-06-04 13 1 17 33 Histopathological Changes on Oyster Crassostrea belcheri Exposed to Subacute Mercury Toxicity <p>Mercury (Hg) is a toxic heavy metal that is ubiquitous environmental contaminants. It leads to biomagnification to food chain. Therefore, mercury poisoning in human can be caused by consuming mercury contaminating diets.&nbsp; Bivalve shellfish, such as oysters, focus on the biomarker for environmental monitoring. In this study, we examine subacute toxicity of mercury to histopathological changes by using light microscope and scanning electron microscope in oyster (<em>Crassostrea belcheri</em>). Two hundred oysters were divided into one control group and four experimental groups. Each experimental group was treated mercuric chloride (HgCl<sub>2</sub>) at different concentrations including 2.5, 5, 10 and 15 µg/l respectively. Each group was randomly sampling every three days for one month. The results found that subacute mercury exposure diminished the occurrence of shell gaping as well as increased mucus secretion. Digestive gland was significant pathological damages. Histopathology showed hemocytes and melanomacrophage centers infiltration, decreasing lipid storage and digestive gland atrophy. Increasing of hemocytes infiltration and gill atrophy were also found. In addition, the concentration of HgCl<sub>2</sub>-exposure at 15 µg/l affected on the most histopathological changes (p&lt;0.05). The present result indicated that the oysters can be used as the biomarker of subacute toxicity of mercury in the environment.</p> Sukanya Phalitakul Sithichon Rattanachan ##submission.copyrightStatement## 2018-07-11 2018-07-11 13 1 35 47 Characterization and Qualification of Vibrio spp. Isolated from Farmed White Seabass in Phra Samut Chedi, Samut Prakan <p>The most common disease that has been reported in estuarine animals is vibriosis, a bacteria of genus vibrio. Generally, the outbreak of vibriosis in fish can be controlled by using antimicrobial agents, however, improper use of medicines in fish farm might lead to overpopulation of antimicrobial resistant bacteria. Therefore, the objective of this study is to survey of bacteria of genus vibrio that can be isolated from 92 samples of farmed white seabass in Phra Samut Chedi, Samut Prakan, which focuses on biological characteristics and profiling of antimicrobial susceptibility pattern against 7 antimicrobial agents including cephalexin, sulfa-trimethoprim, chloramphenicol, tetracycline, nalidixic acid, oxytetracycline and ampicillin, respectively. The result shown that all isolated bacteria grew on a selective medium, TCBS agar. In addition, all isolates were grown properly in a medium enriched by 2% and 4% NaCl. Subsequently, bacteria were identified using a gram staining test and some biochemical tests. It found that all bacterial isolates are gram negative curved-rod and shown oxidase-positive and catalase- positive results, additionally, all sampling bacteria can hemolyse red blood cell on blood agar. From antimicrobial susceptibility test, it found that 82.81% of bacteria are susceptible to tetracycline and chloramphenicol but 78.91% are resistant to ampicillin.</p> Sitthichon Rattanachan Sukanya Phalitakul ##submission.copyrightStatement## 2018-07-11 2018-07-11 13 1 49 59 Efficiency of Low Dose Dopamine on Glomerular Filtration Rate of Dogs with Chronic Renal Failure <p>Normally, low dose dopamine is the popular medication used for treatment of the oliguric and anuric condition in dogs. The effect of low dose dopamine is for increase of diuretic potency resulted in increased elimination of waste products and other substances from the body. However, the evidences to support the diuretic potency of dopamine are inconclusive, especially in dogs with chronic renal failure. Objective of this study was to evaluate the effect of 1 µg/kg/min of dopamine on diuretic induction and increase of glomerular filtration rate (GFR). The records of 13 dogs with chronic renal failure admitted to critical care unit, Kasetsart Veterinary Teaching Hospital, Bangkhen were studied. The creatinine clearance, systolic blood pressure and water out to water in ratio from each dog were used to compare during the period of without and with dopamine administration. The generalized linear model with cross-over analysis was used with the commercial statistical software (NCSS 2007<sup>®</sup>). The results indicated that creatinine clearance during without and with dopamine administration was 0.43 ± 0.25 ml/min and 0.44 ± 0.30 ml/min, respectively (<em>P</em> = 0.9063). Also, systolic blood pressure during without and with dopamine administration was 127.30 ± 20.68 mm. Hg and 129.23 ± 28.71 mm. Hg, respectively (<em>P</em> = 0.7465). Likewise, water out to water in ratio during without and with dopamine administration was 1.01 ± 0.20 and 1.00 ± 0.27, respectively (<em>P</em> = 0.4733). In conclusion, administration of 1 µg/kg/min dopamine had no effect on creatinine clearance, systolic blood pressure and water out to water in ratio. Therefore, dosage of 1 µg/kg/min of dopamine could not increase diuresis and GFR.</p> Komsan Srirattanaprateep Chalermpol Lekcharoensuk ##submission.copyrightStatement## 2018-07-14 2018-07-14 13 1 61 75 Emerging Disease in Tilapia: Tilapia Lake Virus Disease (TiLVD) <p>Tilapia lake virus disease (TiLVD) was reported as an emerging disease in tilapia (<em>Oreochromis</em> spp.) in various regions of the world. TiLV is classified in Orthomyxo-like virus group. Mortality rate was found on fingerling of nile and red tilapia after cage rearing during the first month, tilapia one month syndrome was named for this loss. Affected fish revealed inflammation of the operculum and tail erosion. The developed semi-nested PCR showed more sensitivity response for detection TiLV. Restriction of fish movement, development of vaccine and TiLV resistant tilapia strains should be considered as disease control program.</p> Sumrarn Bunnajirakul ##submission.copyrightStatement## 2018-07-13 2018-07-13 13 1 ึึ77 83 Development of Novel Vaccines Against PRRS Viruses in Pigs <p>Porcine reproductive and respiratory syndrome virus (PRRSV) is a causative agent of a devastating disease that namely Porcine Reproductive and Respiratory Syndrome (PRRS) brought huge losses to pork production in swine industry worldwide. The disease is characterized by massive reproductive failure and late term abortion in sows as well as respiratory disorder in piglets. In the present, PRRS is endemic in several countries in Asia such as China, Japan, South Korea, Taiwan and Thailand. As, PRRSV has become one of the most common viruses affecting swine population, several control strategies were established. However, these viruses still circulate and re-infection occurs in many swine herds. Of all control and prevention measures, vaccination is the main approach for control and eradication of PRRSV infection in swine herds since no specific treatment and no any antiviral drug is available for curing and control of PRRS. Thus, several studies have focused on the development of an effective vaccine against PRRSV in both experimental trials and field studies. Nevertheless, these vaccines still have several drawbacks. Thus, development of an effective PRRSV vaccine is one of the most interesting areas. This present review article provides the knowledge of recent novel PRRSV vaccine development. The information from this review article can be applied for further study about PRRSV vaccine development, resulting in enlighten veterinary practitioners and other related persons to develop more effective control measures aiming to PRRSV eradication.</p> Tippawan Jantafong ##submission.copyrightStatement## 2018-07-13 2018-07-13 13 1 85 99