Journal of Mahanakorn Veterinary Medicine Faculty of Veterinary Medicine, Mahanakorn University of Technology en-US Journal of Mahanakorn Veterinary Medicine 1905-7571 Case Study: Theoretical and Practical Learning Achievement in Animal Subcutaneous Injection of Students in Animal Science Program Faculty of Food and Agricultural Technology Pibulsongkram Rajabhat University <p>The objectives of this research were: 1) to measure the theoretical and practical learning achievement of the students who learned by an integration technique of lecture, demonstration and practice, in animal subcutaneous injection and 2) to measure the satisfaction level of the students on the integration technique of learning. The sample group was specified in 29 sophomore students in Animal Science program in Faculty of Food and Agricultural Technology Pibulsongkram Rajabhat University, who enrolled in the animal hygiene and sanitation subject. The one group pre test-post test design was used for this research. The research tools were the studying document for animal subcutaneous injection, the theoretical and practical learning achievement questionnaire and the learning satisfaction questionnaire. The statistics used for data analysis were percentage, mean, standard deviation and Students <em>t</em>-Test. The result showed that the post-test average learning achievement score of the student (28.52 ± 1.47 points) was higher than the pre-test score (11.00± 0.45 points) (<em>P</em>&lt;0.01). The learning achievement in both the theoretically section and the practical section (8.83 ± 0.66 and 19.67 ± 1.20 points, respectively) were higher than the anticipated standard score at 80% (<em>P</em>&lt;0.01). The overall satisfaction of the students on this technique was high (4.14 ± 0.66 points). These results suggested that the integration technique of lecture, demonstration and practice effectively improved learning achievement.</p> Preecha Moonsan Weerapong Inthong ##submission.copyrightStatement## 2018-09-13 2018-09-13 13 2 101 110 Preliminary Study of Haemosporidian Parasite and White Blood Cell Count in Scotophilus kuhlii Bats <p>Bats played role as a reservoir host of protozoan blood parasite. The objective of this study was to examine the effects of haemosporidian parasite infection and gender on white blood cell (WBC) count of Asiatic lesser yellow house bat (<em>Scotophilus kuhlii</em>) in Muang district, Nakhon Phanom province, Thailand. Forty four adult bats with both sexes and weighing between 17-25 grams were collected their blood samples. Slides of blood smear were stained with Wright-Giemsa stain and observed under a light microscopic. The results showed that the highest percentage of WBC count was seen in neutrophil (55.10±15.79) whereas eosinophil (0.33±0.57) and basophil (0.32±0.56) has the lowest count. The differential WBC count of adult female and male were not different (P&gt;0.05). Similarly, the percentage of WBC count were not different (P&gt;0.05) between positive of infection and negative of infection group. Base on thorough microscopic examination of blood smear, this study observed that prevalence of blood parasites as Haemoproteidae family was 11.36 % (5/44). In conclusion, the results of present study found that an infection of haemosporidian and gender of Asiatic lesser yellow house bats had no effects on WBC count.</p> ชาตรี ชำนาญดี นวรัตน์ ผอบงา ##submission.copyrightStatement## 2018-11-13 2018-11-13 13 2 111 122 Effect of Using Palm Kernel Meal and Saccharomyces cerevisiae as Protein Source for Soybean Meal Replacement in Diet on Nutrients Digestibility, Productive Performance and Carcass Traits in Broiler <p>TThe aim of the study was to investigate effects of palm kernel meal with <em>S. cerevisiae</em> as protein source on nutrient digestibility, productive performance and carcass traits in broilers. There were two experiments in this study. &nbsp;Experiment 1 was the study of effects of palm kernel meal and <em>S. cerevisiae</em> in diet on nutrients digestibility. The results revealed that chemical composition of palm meal with <em>S. cerevisiae</em> consisted of &nbsp;94.87% of dry matter, 19.09% of crude protein, &nbsp;17.83% of crude fiber, 12.06% of fat , 4.07% of ash &nbsp;and &nbsp;2,010 kcal/kg of apparent metabolizable energy in broiler. It was found that coefficients of protein and fiber digestibility were 64.08% &nbsp;and 46.21% , respectively. &nbsp;Experiment 2 was the study of effects of using palm kernel meal with <em>S. cerevisiae</em> in diets on productive performance, carcass traits and foot pad lesion in broilers. A total of 350 birds of one-day-old Ross 308 were divided into five dietary treatments, 7 replications each, 10 chicks per replication. Dietary treatments consisted of Treatment 1: control diet, Treatment 2; control diet using&nbsp; palm kernel meal and <em>S. cerevisiae</em> replaced with soybean meal at 5, 7.5% Treatment 3; control diet using palm kernel meal and S. cerevisiae replaced with soybean meal at &nbsp;10, 15% in starter diet and grower diet, respectively. Treatment 4; treatment 2 supplemented with mannanase enzyme at 0.05% of diet. Treatment 5; treatment 3 supplemented with mannanase enzyme at&nbsp; 0.05% of diet. The results revealed that at 1-17 day of age, average feed intake was not significantly different (P&gt;0.05) but FCR of treatment 4 and 5 were better than treatment 3 (P&lt;0.05). At 18-35 and 1-35 days of age, it was found that average feed intake, weight gain and FCR were not significantly different (P&gt;0.05) among treatment groups. There was no significant difference (P&gt;0.05) in carcass traits and foot pad lesion among treatment groups. In conclusion, palm kernel meal can be used with <em>S. cerevisiae</em> as feed stuff in broiler diet at 5 – 15% without negative effects on productive performance, carcass traits and foot pad lesion in broiler.</p> Pattaraporn Poommarin พิเชษฐ ศรีบุญยงค์ ##submission.copyrightStatement## 2018-11-17 2018-11-17 13 2 123 134 A Retrospective Study of Hematology of Canine Blood Parasite Infections in Kanchanaburi Province, Thailand <p>A retrospective study of canine blood parasite hematological values, the relationship between blood parasite infections and hematological values were from laboratory examination recording at Livestock and Wildlife Hospital during 2012 - 2016. The study showed that the hematological abnormalities in canine blood parasite infections were thrombocytopenia, hypoproteinemia, anemia, hypohemoglobin<span style="text-decoration: line-through;">a</span>emia, and leucopenia (Odds ratio = 7.883, 3.725, 3.178, 2,930 and 1.272). Infected by <em>Ehrlichia canis</em> in canines were thrombocytopenia, hypohemoglobinemia and anemia (Odds ratio = 18.748, 3.187 and 3.180). The presence of <em>Hepatozoon canis </em>in canines were anemia, hypohemoglobinemia and thrombocytopenia (Odds ratio = 2.111, 1.645 and 1.554). The hematological characteristics of canines infected by <em>Babesia vogeli</em> were anemia, hypohemoglobinemia, thrombocytopenia, hypoproteinemia, and leucopenia (Odds ratio = 25.054, 24.097, 14.097, 12.314 and 1.421). These data support that platelet count may increase the reliability of clinical diagnosis for canine blood parasites.</p> วิษณุ วงษ์สว่าง สินีนาถ เจียมทวีบุญ ##submission.copyrightStatement## 2018-12-19 2018-12-19 13 2 135 145 Detection and Identification of Ornithobacterium rhinotracheale from Layer Flocks in Chiang Mai, Thailand <p>Respiratory disease conditions are causing heavy economic losses in the poultry industry. Ornithobacteriosis is one of the infectious diseases of avian species that has been observed in the poultry flocks of many different countries and is continuing to emerge as a growing concern in the economic viability of the poultry industry. The objectives of this study were to detect and identify the ORT from layer flocks in Chiang Mai, Thailand. Layer antibodies were determined in the serum samples of 30 clinically infected layer flocks of different ages (18-63 weeks) by ELISA. The serum analysis showed that the antibody responses were 78% negative, 7% suspect, and 15% positive. Older layers (41-63 weeks) revealed higher positive results than the younger aged layers (18-38 weeks). The bacteria were isolated and identified by polymerase chain reaction (PCR). Bacterial isolation and identification revealed that out of the eleven isolates tested, 8 showed positive results to PCR analysis. This study is very useful and is the first report about ORT found in layer flocks in Chiang Mai where the ORT vaccines are not available. Most importantly, this research has found that both bacteriological and molecular techniques can be used to detect ORT.</p> Sureerat Numee Surachai Pikulkeaw Theeraya Rinut Niwat Chansiripornchai ##submission.copyrightStatement## 2018-12-04 2018-12-04 13 2 147 160 Study on Using Polymerase Chain Reaction and Buffy Coat Smear Method for Trypanosoma evansi Detection in Buffalos of Eastern Thailand <p>The objective of this study was to study on using polymerase chain reaction (PCR) and Buffy coat smear method for <em>Trypanosoma evansi</em> detection in buffalos of eastern Thailand. Samples were collected from buffalo blood during July - December 2016. There were 80 samples; 40 samples from Chachoengsao province and the others were collected from Sa Kaeo province. All the samples were tested by PCR and buffy coat smear method. Packed cell volume (PCV) and plasma protein (PP) of samples were measured and data of husbandry management in each farm were recorded. Results from this study revealed that there was no <em>T. evansi</em> infection in sample from Sa Kaeo province. But, two infected samples of Chachoengsao province were found by both PCR and Buffy coat smear method. Therefore, PCR and buffy coat smear method had 100% sensitivity and 100 % specificity. The prevalence of all samples was 2.5% (2/80). Prevalence of Chachoengsao province was 5% (2/40) while the disease was not detected in Sa Kaeo province. Analysis of husbandry management showed that all of farmers in Chachoengsao and 80 % of farmers in Sa Kaeo province province raise buffalos with other animals. In addition, vector insects were found in almost all buffalos (78/80). The average PCV of samples in Sa Kaeo province (33±2 %) were more than those of Chachoengsao province (29±5 %).The average PP of Chachoengsao province (9.2±0.8 g/dL) were more than Sa Kaeo province (8.8±0.8 g/dL). However,data of husbandry management, vector insects, PCV and PP did not relate to found <em>T. evansi</em> infection in buffalos</p> Kamonporn Panja Thanida Sananmuang Thanakrit Jankong ##submission.copyrightStatement## 2018-12-04 2018-12-04 13 2 161 170 Biodiversity of Blood Sucking Flies associated with the Prevalence of Anaplasma marginale infection in Dairy Farms in Ratchaburi Province <p>The objective of this research was to study the association between the biodiversity of blood sucking flies and prevalence <em>Anaplasma marginale</em> infections in dairy cattle in Ratchaburi Province. A total of 1,419 samples of blood sucking flies was collected from dairy farms of Ratchaburi Province, including 375 samples from Photharam District, 652 samples from Ban Pong District, and 392 samples from Jom Bueng District. The flies were classified by taxonomy and <em>A. marginale</em> was detected, using Polymerase chain reaction (PCR). The blood sucking flies were characterized into 2 families and 3 genus including Muscidae family: genus Stomoxys <em>(Stomoxys calcitrans</em>, <em>S. indicus</em>, and <em>S. sitiens</em>) and genus Haematobia, and Tabanidae family: genus Tabanus (<em>Tabanus rubidus</em> and <em>T. striatus</em>). Blood sucking flies were including associated with the amount of rain and they are mostly found during June to August. According to <em>A. marginale</em> detection, the percentage of <em>A. marginale</em> infection in blood sucking flies 4.75% (68/1,419). Factors influenced the infection of <em>A. marginale</em> in blood sucking flies were diversity of blood sucking flies, season, and the amount of rain (P&lt;0.05). Therefore, for the control, the breeding habitat of these flies should be modified which is around the dairy farms, before the rain season while the surveillance should be arranged to prevent the epidemic caused by blood sucking flies in the area.</p> Nantiya Saetiew Roger W. Stich Sathaporn Jittapalapong ##submission.copyrightStatement## 2019-01-12 2019-01-12 13 2 171 184 Prevalence and Quantity of Gastrointestinal Parasites in Sows of Ban Na Chakwai Famers in Phitsanulok Province <p>The purposes of the study were 1) to study general information of sow farmers and farming conditions and 2) to survey the prevalence of gastrointestinal parasites of sows in Ban Na Chakwai, Phitsanulok province. The general information and farming condition of 20 farmers were collected by interview. Fecal samples were collected from 96 sows to examined parasite eggs by Simple floatation technique, Simple sedimentation technique and Modified McMaster technique. It was found that most of the farmers were female (80.00 %), in age of 50-60 years (60.00 %), raising 1-5 of Large White - Landrace sows/farm (70.00 %). Several types of mixed feed were used: 1) concentrate + broken rice 2) rice bran 3) rice bran + banana stalk + native water spinach and 4) rice bran + food waste. The mixed of rice bran + banana stalk + native water spinach was mostly used (48.96 %).&nbsp; Sows were raised in different floor pens: 1) concrete floor 2) slatted floor (85.42 and 14.58 %, respectively). The prevalence of <em>Isospora suis, Ascaris suum, Strongyloides ransomi and Trichuris suis</em> were 100.00, 10.42, 3.13 and 1.04 %, respectively. The quantity (average egg/oocyte per gram; EPG/OPG) of five species was <em>I. suis</em> (5,330.1±97.9 OPG), <em>A. suum</em> (101.9±5.3 EPG), <em>S. ransomi</em> (9.2±0.7 EPG) and <em>T. suis</em> (1.0±0.1 EPG). It was indicated that, in small farmer condition without parasite control, the mild prevalence of gastrointestinal parasites in sows were common.</p> ปรีชา มูลสาร ยิ่งลักษณ์ มูลสาร จิตราวรรณ เสาเขียว พนิดา จันทรา ไตรภพ แสงแผน ##submission.copyrightStatement## 2019-01-12 2019-01-12 13 2 185 197 Efficiency of Ethanolic Leaf Extract of Solanum torvum Sw. against Staphylococcus spp. from Dog Skin and the Owner Hands. <p><em>Solanum torvum </em>Sw<em>.</em> is an alternative and traditional medicine plant which commonly found in Thailand. The aim of this study was to investigate the antibacterial activity of 95% ethanol crude extract of <em>S. torvum</em> SW. Leaves against pathogenic bacteria isolated from dog skin and owner hands (<em>in vitro</em>). The isolated bacteria were identified based on their biochemical characterization and determined their antibacterial activity of the extracts by using broth microdilution test in microtiter plate 96-well and subculturing into LB rich agar in order to explore the minimal inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC), respectively. The bacterial characterization used in this experiment was specific to the <em>Staphylococcus spp.</em> which revealed <em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>,<em> Staphylococcus sciuri</em> and<em> Staphylococcus chromogenes</em> in both of the sample sources. MIC and MBC were ranged between 3.91- 62.5 mg/ml and showed the greatest antibacterialactivity against<em> Staphylococcus chromogenes</em><em>.</em> This study suggested that the extracts was promising for development in the treatment against pathogenic bacterial skin diseases in the future.</p> ณัฐกาญจน์ นายมอญ ธิติชัย จารุเดชา อารยา สืบขำเพชร ##submission.copyrightStatement## 2019-01-22 2019-01-22 13 2 199 211 Feline Trichomonosis <p>Feline trichomonosis, a large bowel disease in domestic cats caused by <em>Trichomonas foetus</em>, is now prevalent in many parts of the world. The disease can cause asymptomatic infection in cats or chronic diarrhea which mainly occurring in kittens.&nbsp; <em>T. foetus</em> is a protozoan parasite which has long been recognized as a causative agent of bovine trichomonosis, a disease of cattle reproductive system.&nbsp; While bovine trichomonosis is a venereal disease in cattle, feline trichomonosis is thought to transmit by faecal-oral route.&nbsp; This review article provides concept knowledge of the disease in cats, the disease transmission, diagnosis and treatment.</p> จิตรบรรจง ทุมพงษ์ ##submission.copyrightStatement## 2019-01-22 2019-01-22 13 2 213 224