Screening and Identification of Endophytic Bacteria from Organic Red Jasmine Rice Soil

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สมคิด ดีจริง อนุตตรีย์ บุญต่อ

Abstract

Microorganisms have relationship with plants. Endophytic bacteria colonize inner plant tissue without damaging. These bacteria show an important role in plant growth promotion.  Thus, they can contribute to increase efficiency of organic based system of rice Intensification (SRI). The purpose of this study to analyze soil  properties, screen and identify of phosphate solubilizing and indole acetic acid (IAA) endophytic bacteria from organic red jasmine rice tissue. It was found that ten years organic soil have low to moderate content of organic matter, available phosphorus and exchangeable potassium, bulk density, field capacity  and saturated hydraulic conductivity. Endophytic bacteria, RSPVK-2, RRPVK-4, RRTSA-5, RRTSA-6, RLISP2-1, RRTSA-3, RRPVK-1, RRTSA-1 and RSISP2-1 solubilized phosphate in PVK broth at 191.78, 92.65, 91.80, 73.22, 73.17, 71.23, 70.93, 69.93 and 67.49 mgPO42-/L, respectively. Also, bacteria RSTSA-6 could produce indole-3-acetic acid (IAA) 20.55 mg/L.  The genetic sequencing on 16S rRNA of RSPVK-2, RRPVK-4, RRTSA-6, RLISP2-1, RRTSA-1 and RSISP2-1 had similar to Burkholderia cenocepacia, Paenibacillus favisporus, Paenibacillus alvei, Bacillus thuringiensis, Bacillus subtilis and Ralstonia pickettii, respectively. RRTSA-5, RRTSA-3, RRPVK-1 were identified as Bacillus pumilus.

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