Selection of free living nitrogen fixing bacteria to promote on rice growth
The objective of this research was to selected free-living nitrogen fixing bacteria to promote on rice growth, which isolated bacteria from rice rhizosphere soil samples. The result of bacteria isolation found that there were 310 isolates and the nitrogen fixing bacteria were found 26 isolates. Testing the effectiveness of the nitrogen fixation of bacteria and IAA production, the result showed 10 isolates. Identification of selected bacteria by analyzing the 16S rRNA genes sequence indicated three isolates i.e., CM2-2 Bacillus altitudinis, LP1-5 Bacillus aryabhattai and PY7-6 Pseudomonas nitroreducens, which produced high nitrogen compounds at 15.60±0.40, 7.70±0.70 and 6.30±0.10 mg/L respectively, In addition, PY7-6 Pseudomonas nitroreducens also produced the highest IAA at 23.90±0.20 mg/L. Experimental inoculating with NPK chemical fertilizers were performed in rice grown in pots during the seedling period of 30 days. The result showed that the inoculations of CM2-2 Bacillus altitudinis with NPK were the best in affecting the rice growth, i.e., the height of the rice, root length, number of leaves and fresh and dry weight. This result showed significant differences when compared to the control. However, the determination of nitrogen contents in rice did not display differences from the control, but that CM2-2 Bacillus altitudinis+NPK was likely better than others. This study is the first report in Thailand to show the CM2-2 Bacillus altitudinis, and the effective free-living nitrogen fixing bacteria strains to promote the rice growth. This can be developed as a bio-fertilizer.
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