Inter simple sequence repeat (ISSR) based analysis of morphological variation of transgenic Kalanchoe (Kalanchoe blossfeldiana)

  • Pheravut Wongsawad Department of Biology, Faculty of Science, Chiangmai University, Chiangmai Province 50200
  • Chutima Jaiphet Department of Biology, School of Science, University of Phayao, Phayao Province 56000
  • Yaowalak Saenprasit Department of Biology, School of Science, University of Phayao, Phayao Province 56000
  • Sopapun Jaiwut Department of Biology, School of Science, University of Phayao, Phayao Province 56000
  • Amolwan Haohan Department of Biology, School of Science, University of Phayao, Phayao Province 56000
  • Phopgao Puttharak Department of Biology, School of Science, University of Phayao, Phayao Province 56000
Keywords: Transgenic Kalanchoe (Kalanchoe blossfeldiana), inter simple sequence repeat, polymerase chain reaction

Abstract

Inter simple sequence repeats (ISSR) analysis, using 10 primers was performed to examined morphological variation among 26 transgenic Kalanchoe (Kalanchoe blossfeldiana). The study of ISSR polymorphism was considered transgenic Kalanchoe into the polyphyletic group of its UPGMA tree, and analyzed with distance method (UPGMA) in PAUP 4.0b10, 5,000 replicates which comprised of 2 clades. Meanwhile, the first clade showed that sample 9 and 10 have close relationships support with 52% bootstrap, of their pointed tip. Simultaneously, the second clade revealed sample 18 and 19 have close relationships support with 76% bootstrap, of theirs oval-shaped petals and pointed tips, the sample 24 and 25 have close relationships support with 58% bootstrap that have close relationships with sample 21 support with 68% bootstrap, of theirs reddish pink flowers and pointed tips and the sample 22 and 23 have close relationships support with 75% bootstrap, with theirs reddish orange flowers, oval-shaped petals and pointed tips.

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Published
2019-08-30
How to Cite
1.
Wongsawad P, Jaiphet C, Saenprasit Y, Jaiwut S, Haohan A, Puttharak P. Inter simple sequence repeat (ISSR) based analysis of morphological variation of transgenic Kalanchoe (Kalanchoe blossfeldiana). Naresuan Phayao Journal [Internet]. 30Aug.2019 [cited 21Nov.2019];12(2):28-1. Available from: https://www.tci-thaijo.org/index.php/journalup/article/view/200755
Section
Research articles