สภาวะที่เหมาะสมสำหรับการงอกของสปอร์และความไวต่อสภาวะเครียดของ Talaromyces marneffei

  • Aksarakorn Kummasook Division of Clinical Microbiology and Medical Parasitology, Department of Medical Technology, School of Allied Health Sciences, University of Phayao, Phayao, Thailand 56000

Abstract

The purpose of the study was to investigate the conditions for spore germination under environmental stress to determine susceptibility of T. marneffei to conditions that initiate dimorphism. The results revealed that percentage of spore germination at 25oC is higher than germination at 37oC, and constants at 16 hours after inoculation as compared to 20 hours, respectively. Additionally, sublethal dose concentration testing demonstrated that both mycelium and yeast phase was susceptible to the sublethal dose of CaCl2 (200 mM), NaCl (0.5M), Sorbitol (0.75M), Menadione (62.5 µM), H2O2 (2.5 mM), and SDS (0.005 %).  Meanwhile, the sublethal dose of t-butyl hydroperoxide in mold and yeast phases were 1.5 and 0.5mM, respectively.  This result could be further applied for fungal stress susceptibility testing comparing of wild type and mutants.

Published
2019-12-27
How to Cite
1.
Kummasook A. สภาวะที่เหมาะสมสำหรับการงอกของสปอร์และความไวต่อสภาวะเครียดของ Talaromyces marneffei. Naresuan Phayao Journal [Internet]. 27Dec.2019 [cited 21Jan.2020];12(3):31-7. Available from: https://www.tci-thaijo.org/index.php/journalup/article/view/222468
Section
Research articles