Antibacterial Activity of Ethanolic Extract of Trisamo Against Diarrheal Pathogen

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Asma Asae Puwadon Meemak Benjawan Poonthananiwatkul Rosenanee Hemtrakoonwong Suhaila Sama-ae

Abstract

The objective of this research was to study the antibacterial activities of ethanolic extract of Trisamo against diarrhea caused bacteria (Escherichia coli and Salmonella spp.). Trisamo extract included Terminalia chebula Retz., Terminalia arjuna Roxb. and Terminalia bellirica (Gaertn.) Roxb. was tested on their antimicrobial
activities by using agar disc diffusion method. For evaluating Minimum Inhibitory Concentration
(MIC) and Minimum Bactericidal Concentration (MBC), macrodilution method was applied in this study and three experiments were repeated for each test to get an average. The results of this study showed that yield percent of crude ethanolic extract was 19.09. The average of inhibition zone for Escherichia
coli (E. coli) and Salmonella spp. from Trisamo extract presented as 9.34 ? 0.58 mm. and 9.00 ? 0.00 mm, respectively. MIC for E. coli and Salmonella spp. were 256 ?g/ml and 512 ?g/ml and MBC for both were > 1024 ?g/ml respectively. In comparison MBC of Trisamo extract with norfloxacin as standard, MBC of norfloxacin demonstrated as 16 ?g/ml that was more effective than Trisamo extract as 16 times.

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บทความวิจัย

References

Amanullah, S., Chandramoorthy, H. C., Kumar, V. A. & Khatheeja, S. (2010). Antimicrobial activity of Triphala against bacterial isolates from HIV infected patients. Jundishapur Journal of Microbiology, 4(12), 9 - 17.
Aneja, K. R., Sharma, C. & Joshi, R. (2012). Antimicrobial activity of Terminalia arjuna Wight & Arn.: An ethnomedicinal plant against pathogens causing ear infection. Brazilian Journal of otorhinolaryngology, 78(1), 68 - 74.
Art of healing. (1998). Traditional Medicine Textbook of Pharmacy. [n.p.]. (in Thai)
Board of committee of the strategic development plan for the national health no. 12. (2016). the strategic development plan for the national health no. 12. [n.p.]. (in Thai)
Bureau of Epidemiology. (2016). Annual epidemiology surveillance report 2015. Bangkok: Printing of Agricultural Co-operative Federation of Thailand. (in Thai)
Chanda, S., Menpara, D. & Desai, D. (2013). Antimicrobial Activity of Terminalia bellerica Leaf and Stem Collected from Two Different Sites. American Journal of Phytomedicine and Clinical Therapeutics, 1(9), 721 - 733.
Debnath, S., Dey, D., Hazra, S., Ghosh, S., Ray, R. & Hazra, B. (2013). Antibacterial and antifungal activity of Terminalia arjuna Wight & Arn. bark against multi-drug resistant clinical isolates. Journal of Coastal Life Medicine, 1(4), 312 - 318.
Devi, P. N., Kaleeswari, S. & Poonkothai, M. (2014). Antimicrobial activity and phytochemical analysis of fruit extracts of Terminalia Bellerica. International Journal of Pharmacy and Pharmaceutical Sciences, 6(5), 639 - 642.
Intranupakorn, R. (2007). Direction and extraction of herb. Bangkok: Chulalongkorn university. (in Thai)
Kannan, P., Ramadevi, S. R. & Hopper, W. (2009). Antibacterial activity of Terminalia chebula fruit extract. African Journal of Microbiology Research, 3(4), 180 - 184.
M Alam Morshed
M Alam Morshed
Morshed, M. A., Uddin, A., Rahman, A., Hasan, T., Roy, S., Amin, A. A., et al. (2011). In vitro antimicrobial and cytotoxicity screening of Terminalia arjuna ethanol extract. International Journal of Biosciences, 1(2), 31 - 38.
Murad, F., Alam, K. A., Mazumdar, R. M., Islam, S., Nipa, M. N., Iqbal, A., et al., (2011). Anti-bacterial Activity of the Extract of Terminalia arjuna Against Multi Antibiotic Resistant Vibrio cholera. Journal of Scientific Research, 3(1), 129 - 137.
Natha, N., et al. (Ed.). (2015). Introduction to clinical medicine. Bangkok: Book project of Phramongkutklao college of medicine. (in Thai)
Prompamorn, P. & Ratcharin, N. (2016). Evaluation of Antibacterial Activity and Gel Formulation Development from Terminalia chebula Retz Extract. Research Journal Sciences and Technology, 9(1), 51 - 64. (in Thai)