PREVALENCE AND ND1 IDENTIFICATION OF TREMATODE, Echinostoma revolutum IN METACERCARIA AND ADULT STAGES FROM LAMPHUN AND LAMPANG PROVINCES, THAILAND

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Patthanan Sakda Pheravut Wongsawad Chalobol Wongsawad

Abstract

            Echinostome metacercariae are the infective stage of echinostome flukes that can be found in fresh water snails, tadpoles, and fish. In this study, echinostome metacercariae from three species of Filopaludina snails; Filopaludina martensi martensi, F. dorliaris and F. summatrensis polygramma were collected from four districts of Lamphun and Lampang Provinces and the prevalence of metacercrial infection by the three species was 27.05%, 41.92% and 48.05% respectively. The adult flukes were covered from experimentally infected hamsters and chickens at the 14th day after infection and were identified to species using light microscopy (LM), scanning electron microscopy (SEM), and a phylogenetic tree for the mitochondrial gene ND1 (nicotinamide adenine dinucleotide dehydrogenase subunit 1). This fluke has an elongated body, entrally curved body , head collar with 37 prominent collar spines, and 2 type of testes shape including lobed and roudned. However, identification of Echinostoma revolutumin this present study has been based on morphology, morphometric data, and the phylogenetic tree from ND1 subunit The results confirmed status of E. revolutum in Filopaludina spp. snails as the natural second intermediate host and also demonstrated the occurrence of this intestinal flukes in Lamphun and Lampang provinces.

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