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The objective of this study was to enhance methane production by anaerobic co-digestion of vinasse and molasses in batch fermentation. Kinetic reaction of methane production at optimum conditions was further studied in order to estimate hydraulic retention time (HRT) and organic loading rate (OLR) for continuous fermentation. Results revealed that the co-digestion of vinasse and molasses enhanced methane production efficiency and chemical oxygen demand (COD) removal efficiency in comparison to the mono-digestion of vinasse. The optimum ratio of vinasse to molasses and substrate concentration were 3:2 and 10 g-volatile solid (VS)/L, respectively. The maximum cumulative methane production and methane yield of 3,847 mL/L and 385 mL/g-VS, respectively, were obtained. COD removal efficiency of 91.59% was achieved. The study on kinetic reaction of methane production at the optimum conditions revealed that reaction rate constant (k) obtained from the first order reaction was 0.135 d-1 at the estimated HRT and OLR of 30 d and 0.34 g-VS/L.d, respectively, were obtained.
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