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Very Low Density Lipoprotein/Vitellogenin (VLDL/VTG)receptor gene plays crucial roles in laying hen production.In this study, we investigated genetic polymorphism of the VLDL/VTG receptor gene in four chicken breeds:-Rhode Island Red (RR), Single-Comb White Leghorn (WL), Commercial Breeds (HyLine brown chicken, HL)and Hybrid Red Jungle Fowl (Kai-Tor). PCR technique was used to amplify VLDL/VTG receptor gene with three specific primers that were designed to complement the unique 5’flanking region of VLDL/VTG receptor gene at location of -1351to -1599.One SNPs (A-1393G)was identified in the 5´flanking region of VLDL/VTG receptor gene. The association of these SNPs with egg traits and growth performance were examined.The body weight (BW), egg weight at first egg (EW) and b-value of shell color of the AA genotype were significantly higher than those of the GG genotype (p<0.05).In contrast, the total eggshell color (ESC)and L-value of shell color of the AA genotype were significantly lower than those of the GG genotype (p<0.05).Positive phenotypic correlations were found among BW and EW (0.712), BW and a-value (0.435), EW and a-value (0.642).In addition, negative phenotypic correlations were found among a-value and L-value (-0.833), ESC and BW (-0.320), and ESC and EW (-0.545) were negative.This paper confirmed that VLDL/VTG receptor could be a candidate gene related to egg traits and growth performance
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Baylan M, Celik LB, Akpinar GC. et al. Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders, Revista Brasileira de Zootecnia. 2007; 46, 5-12.
Bujo H, Hermann M, Kaderli MO. et al. Chicken oocyte growth is mediated by an eight ligand binding repeat member of the LDL receptor family, The Embo Journal. 1994; 13:65-75.
Bujo H, Yamamoto T, Hayashi K. et al. Mutant oocytic low density lipoprotein receptor gene family member causes atherosclerosis and female sterility, Proceedings of the National Academy of Sciences. 1995; 92: 5-9.
Cao DG, Zhou Y, Lei QX. et al. Associations of very low density lipoprotein receptor (VLDLR) gene polymorphisms with reproductive traits in a Chinese indigenous chicken breed, Journal of Animal and Veterinary Advances. 2012; 11: 62-67.
Nimpf J, Schneider, WJ. Receptor-mediated lipoprotein transport in laying hens, The Journal of Nutrition. 1991; 121: 71-74.
Novak S, Hiesberger T, Schneider WJ. et al. A new low density lipoprotein receptor homologue with 8 ligand binding repeats in brain of chicken and mouse, Journal of Biological Chemistry. 1996; 271: 32- 36.
Rodriguez JN, Bon E, Menn FL. Vitellogenein receptor during vitellogenesis in the rainbow trout (Oncorhynchusmykiss), The Journal of experimental Zoology. 1996; 274: 63-70.
Shen X, Steyrer E, Retzek H. et al. Chicken oocyte growth: receptor-mediated yolk deposition, Cell and Tissue Research. 1993; 272: 59-71.
Yaemkong, S. Diversity of phenotypic characteristics of Thai indigenous chickens in Phitsanulok province, Rajabhat Journal of Sciences, Humanities & Social Sciences. 2014; 15: 63-73.
Takahashi S, Kawarabayasi Y, NaKai T. t al. Rabbit very low density lipoprotein receptor: A low density lipoprotein receptor-like protein with distrinct ligand specificity, Proceeding of the National Academy of Sciences U.S.A. 1992; 89: 52-56.
Takahashi S, Sakai J, Fujino T. et al. The very low density lipoprotein (VLDL) receptor–a peripheral lipoprotein receptor for remnant lipoproteins into fatty acid active tissues. Molecular and Cellular Biochemistry. 2003; 248: 21-27.
Wang C, Li SJ, Yu WH. et al. Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anasplatyrhynchos). Genetics Selection Evolution. 2011; 43: 29.
Wyne KL, Pathak RK, Seabra MC, et al. Expression of the VLDL receptor in endothelial cells, Arteriosclerosis, Thrombosis, and Vascular Biology. 1996; 16: 7-15.
Wu Y, Pi JS, Pan AL.et al. Two novel linkage SNPs of VLDLR gene intron 11 are associated with laying traits in two quail populations, Archivfuer Tierzucht. 2015; 58: 1-6.
Yagyu H, Lutz EP, Kako Y. et al. Very low density lipoprotein (VLDL) receptor-deficient mice have reduced lipoprotein lipase activity possible causes of hypertriglyceridemia and reduced body mass with VLDL receptor deficiency, Journal of Biological Chemistry. 2002; 277: 37-43.
Yao JF, Chen ZX, Xu GY. et al. Low-density lipoprotein receptor-related protein 8 gene association with egg traits in dwarf chickens, Poultry Science. 2010; 89: 83-86.
Zhan H, Feng Y, Shen S. et al. Polymorphisms of chicken oocyte vitellogenesis receptor gene (ovr) and association with egg traits of chickens, Journal of Agricultural Biotechnology. 2009; 17: 67-71.
Zhang K, Qin Z, Chen T. et al. HapBlock: haplotype block partitioning and tag SNP selection software using a set of dynamic programming algorithms, Bioinformatics. 2004; 21: 31-34.