Skeletal Muscle Studying in Non-skeletal Disease Thai Population: Cadaveric Cases

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Jariya Waisayarat
Arpa Pornpetchpracha
Mana Rochanawutanon

Abstract

Objectives: To present normative data on muscle fiber type proportion and mean fiber size of the biceps brachii and vastus lateralis muscles in non-skeletal muscle disease Thai population.


Methods: Muscle samples for each of the left biceps brachii (n = 20) and the left vastus lateralis (n = 20) muscle were taken from 20 cadavers (14 males and 6 females) within 18 to 36 hours after sudden accidental death or who had died after a short acute illness without evidence of neuromuscular disease. Three fiber types (type I, type IIA, and type IIB) were classified using myofibrillar ATPase histochemistry. Fiber type proportion and the lesser diameter of muscle fiber type I, type IIA and type IIB was measured. The mean lesser diameter of each fibers was analyzed statistically comparing between males and females, between age group less than 50 years old (< 50 yrs) and age group 50 years old more (³ 50 yrs), and between biceps brachii and vastus lateralis muscles.


Results: The fiber type percentage of biceps brachii and vastus lateralis muscle showed predominance of type II fiber and on significance differences between type IIA, and type IIB proportions. There were no significant difference in the percentage of each type between males and females. The mean lesser fiber diameter of biceps of biceps brachii and vastus lateralis muscles in our study was in normal range established by previous standard reference. Type I fiber were larger than type II fibers, and type IIA fibers were larger than type IIB fibers in males and females. In vastus lateralis muscle, age group less than 50 years old (< 50 yrs) had larger fibers size than age group 50 years old more (³ 50 yrs) in all type of muscle fibers.


Conclusion: There were no gender difference in the fiber size and fiber proportion in our study. The mean lesser fiber diameter of type I, type IIA and type IIB in non-skeletal muscle disease Thai population was with the interval established from the data published by previously standard reference. Aging had influence on muscle fiber by decrease fiber size in all three types in vastus lateralis muscle.

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How to Cite
1.
Waisayarat J, Pornpetchpracha A, Rochanawutanon M. Skeletal Muscle Studying in Non-skeletal Disease Thai Population: Cadaveric Cases. Rama Med J [Internet]. 2011 Dec. 26 [cited 2024 Mar. 29];34(4):190-8. Available from: https://he02.tci-thaijo.org/index.php/ramajournal/article/view/137875
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Original Articles

References

Dubowitz V. Muscle Biopsy: A Practical Approach. 3rd ed. London: 3rd ed. London: Baillière Tindall; 2007.

Anderson JR. Recommendations for the biopsy procedure and assessment of skeletal muscle biopsies. Virchows Arch. 1997;431(4):227-33.

Klein CS, Marsh GD, Petrella RJ, Rice CL. Muscle fiber number in the biceps brachii muscle of young and old men. Muscle Nerve. 2003;28(1):62-8. doi:10.1002/mus.10386.

Ng THK, Leung SY. Muscle biopsy: an overview. J Hong Kong. Med Assoc. 1991;43:21-5.

Magistris MR, Kohler A, Pizzolato G, Morris MA, Baroffio A, Bernheim L, et al. Needle muscle biopsy in the investigation of neuromuscular disorders. Muscle Nerve. 1998;21(2):194-200.

Mattiello-Sverzut AC, Chimelli L, Moura MS, Teixeira S, de Oliveira JA. The effects of aging on biceps brachii muscle fibers: a morphometrical study from biopsies and autopsies. Arq Neuropsiquiatr. 2003;61(3A):555-60. doi:10.1590/s0004-282x2003000400006.

Behan WM, Cossar DW, Madden HA, McKay IC. Validation of a simple, rapid, and economical technique for distinguishing type 1 and 2 fibres in fixed and frozen skeletal muscle. J Clin Pathol. 2002;55(5):375-80. doi:10.1136/jcp.55.5.375.

Brooke MH, Engel WK. The histographic analysis of human muscle biopsies with regard to fiber types. 1. Adult male and female. Neurology. 1969;19(3):221-33. doi:10.1212/wnl.19.3.221.

Shorey CD, Cleland KW. Morphometric analysis of frozen transverse sections of human skeletal muscle taken post-mortem. Acta Anat (Basel). 1988;131(1):30-4. doi:10.1159/000146481.

Mattiello-Sverzut AC, Chimelli L, Teixeira S, Pierre M, Oliveira L. Effects of chronic heart disease on skeletal muscle fiber size. Braz J Med Biol Res. 2005;38(2):303-7. doi:10.1590/s0100-879x2005000200019.

Slavin G, Sowter C, Ward P, Paton K. Measurement of striated muscle fibre diameters using interactive computer-aided microscopy. J Clin Pathol. 1982;35(11):1268-71. doi:10.1136/jcp.35.11.1268.

Staron RS, Hagerman FC, Hikida RS, Murray TF, Hostler DP, Crill MT, et al. Fiber type composition of the vastus lateralis muscle of young men and women. J Histochem Cytochem. 2000;48(5):623-9. doi:10.1177/002215540004800506.