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The objective of current study was to evaluate an anti-oxidant activity with different solvent polarities (methanol, ethyl acetate, dichloromethane, and hexane) derived from stem of Piper ribesioides together with stalk and seed of Zanthoxylum limonella traditional herbal medicine in Northern Thailand. Natural antioxidant in medicinal plants were defined for provide antioxidant activity by DPPH scavenging assay together with quantification of the phenolic and flavonoid constituents using Folin Ciocalteau and Aluminium chloride method, respectively. The polar methanol extract showed greatest antioxidant effect, while ethyl acetate was the second order reaction both in P. ribesioides and Z. limonella. The ability of methanol extract from stem of P. ribesioides exhibited strongest antioxidant DPPH radical scavenging with IC50 values as 0.2 mg/ml, which was correlated with maximum value of total phenolic acid (68.83 0.38 mg equivalent of gallic acid per gram, mgGAE/g) and flavonoid content (37.13 0.47 mg equivalent of quercetin per gram, mgQE/g). Similarly, the highest anti-oxidant activity of Z. limonella stalk and seed extract on methanol extract showed IC50 values as 0.26 and 0.37 mg/ml, respectively. Stalk showed higher phenolic content (53.67 0.45 mgGAE/g) than seed (24.15 0.48 mgGAE/g) as well as total flavonoid content of stalk (25.76 0.43 mgQE/g) and seed (10.25 0.63 mgQE/g). A relationship between antioxidant activities and increasing levels of total phenolic compounds -or flavonoids was found to be active based on solvent polarities in the extraction of these medicinal plants. Our findings revealed the preliminary scientific evidence for develop traditional herbal medicine into a valuable source for the future of human health care.
2. Mah SH, Teh SS, Ee GC. Anti-inflammatory, anti-cholinergic and cytotoxic effects of Sida rhombifolia. Pharm Biol. 2017 Dec;55(1):920-8.
3. Papas AM. Diet and antioxidant status. Food Chem Toxicol. 1999 Sep-Oct;37(9-10):999-1007.
4. Bakkalbasi E, Mentes O, Artik N. Food ellagitannins-occurrence, effects of processing and storage. Crit Rev Food Sci Nutr. 2009 Mar;49(3):283-98.
5. Fantini M, Benvenuto M, Masuelli L, Frajese GV, Tresoldi I, Modesti A, et al. In vitro and in vivo antitumoral effects of combinations of polyphenols, or polyphenols and anticancer drugs: perspectives on cancer treatment. Int J Mol Sci. 2015 Apr 24;16(5):9236-82.
6. Kaur G, Alam MS, Jabbar Z, Javed K, Athar M. Evaluation of antioxidant activity of Cassia siamea flowers. J Ethnopharmacol. 2006 Dec 6;108(3):340-8.
7. Toda S. Antioxidative effects of polyphenols in leaves of Houttuynia cordata on protein fragmentation by copper-hydrogen peroxide in vitro. J Med Food. 2005 Summer;8(2):266-8.
8. Gonda R, Takeda T, Akiyama T. Studies on the constituents of Anaxagorea luzonensis A. GRAY. Chem Pharm Bull (Tokyo). 2000 Aug;48(8):1219-22.
9. Gulcin I. The antioxidant and radical scavenging activities of black pepper (Piper nigrum) seeds. Int J Food Sci Nutr. 2005 Nov;56(7):491-9.
10. Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK, et al. Piper Species: A Comprehensive Review on Their Phytochemistry, Biological Activities and Applications. Molecules. 2019 Apr 7;24(7).
11. Virinder S, Parmar, Subhash C, Jain, Bisht S, Jain R, et al. Phytochemistry of genus Piper Phytochemistry. 1997;46(4):597-673.
12. Salleh WM, Hashim NA, Ahmad F, Heng Yen K. Anticholinesterase and antityrosinase activities of ten piper species from malaysia. Adv Pharm Bull. 2014 Dec;4(Suppl 2):527-31.
13. Watcharawit R, Soonwera M. Pediculicidal effect of herbal shampoo against Pediculus humanus capitis in vitro. Trop Biomed. 2013 Jun;30(2):315-24.
14. Phankaen Y, Pluempanupat W, Mourad AK, Bullangpoti V. Bioefficacy of Piper Ribesioides (Piperaceae) Extracts against Nilaparvata Lugens Stal. (Homoptera: Delphacidae). Commun Agric Appl Biol Sci. 2014;79(2):229-32.
15. Charoensup R, Duangyod T, Phuneerub P, Singharachai C. Pharmacognostic specification of Zanthoxylum limonella (Dennst.) Alston: Fruits and seeds in Thailand. J Adv Pharm Technol Res. 2016 Oct-Dec;7(4):134-8.
16. Das NG, Dhiman S, Talukdar PK, Rabha B, Goswami D, Veer V. Synergistic mosquito-repellent activity of Curcuma longa, Pogostemon heyneanus and Zanthoxylum limonella essential oils. J Infect Public Health. 2015 Jul-Aug;8(4):323-8.
17. Pitasawat B, Champakaew D, Choochote W, Jitpakdi A, Chaithong U, Kanjanapothi D, et al. Aromatic plant-derived essential oil: an alternative larvicide for mosquito control. Fitoterapia. 2007 Apr;78(3):205-10.
18. Nakagawa K, Promjareet A, Priprem A, Netweera V, Hara H. Investigation of scavenging activities and distribution of paramagnetic species in Zanthoxylum limonella seeds. Free Radic Res. 2016 Dec;50(12):1432-40.
19. Palasuwan A, Soogarun S, Lertlum T, Pradniwat P, Wiwanitkit V. Inhibition of Heinz Body Induction in an inVitro Model and Total Antioxidant Activity of Medicinal Thai Plant. Asian Pacific J Cancer Prev. 2005;6:458-63.
20. Nanasombat S, Wimuttigosol P. Antimicrobial and Antioxidant Activity of Spice Essential Oils. Food Sci Biotechnol. 2011 Feb;20(1):45-53.
21. Tangjitjaroenkun J, Chavasiri W, Thunyaharn S, Yompakdee C. Bactericidal effects and time-kill studies of the essential oil from the fruits of Zanthoxylum limonella on multi-drug resistant bacteria. J Essent Oil Res. 2012;24(4):363-70.
22. Stanojevic L, Stankovic M, Nikolic V, Nikolic L, Ristic D, Canadanovic-Brunet J, et al. Antioxidant Activity and Total Phenolic and Flavonoid Contents of Hieracium pilosella L. Extracts. Sensors (Basel). 2009;9(7):5702-14.
23. Ahmad A HA, Mujeeb M, Khan AS, Alhadrami AAH, Bhandari A. Quantification of total phenol, flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits. Asian Pac J Trop Biomed. 2015;5(2):101-07.
24. Upadhya V, Pai SR, Sharma AK, Hegde HV, Kholkute SD, Joshi RK. Compound Specific Extraction of Camptothecin from Nothapodytes nimmoniana and Piperine from Piper nigrum Using Accelerated Solvent Extractor. J Anal Methods Chem. 2014;2014:932036.
25. Shanmugapriya K SPS, Payal H, Mohammed SP, Williams B. Antioxidant potential of pepper (Piper nigrum Linn.) leaves and its antimicrobial potential against some pathogenic microbes. Indian J Nat Prod Resour. 2012;3(4):570-77.
26. Uyoh A, Chukwurah N, Akarika C, Antia A. Potentials of Two Nigerian Spices—Piper nigrum and Monodora myristica as Sources for Cheap Natural Antioxidant. Am J Plant Sci. 2013;4:1105-15.
27. Supabphol R TJ. Chemical Constituents and Biological Activities of Zanthoxylum limonella (Rutaceae): A Review. Trop J Pharm Res. 2014;Dec;13(12):2119-30.
28. Snelgrove D, Lusztyk J, Banks J, Mulder P, Ingold K. Kinetic Solvent Effects on Hydrogen-Atom Abstractions: Reliable, Quantitative Predictions via a Single Empirical Equation. J Am Chem Soc 2001;123:469-77.
29. Geetha E, Irulandi K, Mehalingam P. Evaluation of antioxidant and free radical scavenging activities of different solvent extracts of leaves of Piper umbellatum. Asian J Pharm Clin Res. 2017;10(2):274-76.
30. Nahak G, Sahu R. Phytochemical Evaluation and Antioxidant activity of Piper cubeba and Piper nigrum. J Appl Pharm Sci. 2011;8:153-7.
31. Reichardt C WT. Solvents and Solvent Effects in Organic Chemistry. Edition T, editor. Germany2005.
32. Prochazkova D, Bousova I, Wilhelmova N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011 Jun;82(4):513-23.
33. Radzali M, Nur Dalila Hani MZ. Antioxidant properties of leaf crude extracts of Piper nigrum L. Proceedings of the Seminar on Medicinal and Aromatic Plants, (MAPS 2008) AGRIS. 2009.
34. Dugas AJ, Castaneda-Acosta J, Bonin GC, Price KL, Fischer NH, Winston GW. Evaluation of the total peroxyl radical-scavenging capacity of flavonoids: structure-activity relationships. J Nat Prod. 2000 Mar;63(3):327-31.
35. Pietta P. Flavonoids as antioxidants. J NatProd. 2000;63:1035-42.
36. Luanchoy S TS WY, and Temsiririrkkul R. Antioxidant Activity of a Thai Traditional Formula for Longevity. Mahidol University Journal of Pharmaceutical Sciences. 2014;41(3):1-5.
37. Supabphol RT. Chemical Constituents and Biological Activities of Zanthoxylum limonella (Rutaceae): A Review. . Trop J Pharm Res. 2014;13(12):2119-30.
38. Zakhari S. Overview: how is alcohol metabolized by the body? Alcohol Res Health. 2006;29(4):245-54.