Analysis of bioactive compounds, polysaccharides and antioxidant activity in different parts of Dendrobium ‘Sonia Jo Daeng’

Main Article Content

Kullanart Obsuwan Jeong Byoung Ryong Sarunyaporn Maksup

Abstract

Numbers of research studies have revealed the potency of bioactive substances from Dendrobium spp. especially in East Asia. However, only limited information on detected bioactive compounds from the Thai Dendrobium hybrids, therefore, this research aimed to analyze the amount of some bioactive compounds, polysaccharides, and antioxidant activity from leaves, stems, roots, flowers and peduncles of 1- and 4-year-old Dendrobium ‘Sonia Jo Daeng’. The studies showed that the highest total phenolic compounds were found in the flowers of 1-year-old plants (7.23±0.80 mg GAE/g DW) followed by roots, flowers and leaves of 4-year-old plants (6.79±1.88, 6.63±0.67, and 6.07±0.62 mg GAE/g, respectively). The maximum anthocyanin level was found in the flowers of 1- and 4-year-old plants (13.28±2.09 and 10.21±6.35 mg/l, respectively). The polysaccharide was highest in flowers of 1-year-old plants (153.95±12.63 mg glucose/g polysaccharide). The highest antioxidant activity was found in the flowers of 1-year-old plants (6.12±0.52 mg trolox/g DW) as well as in roots of 4-year-old plants (5.89±1.31 mg trolox/g DW). The results indicated that antioxidant activities were related to amounts of total phenolic compounds, contents of anthocyanin and polysaccharides.

Keywords

Article Details

Section
Research Articles

References

Albalasmeh, A. A., Berhe, A. A., and Ghezzehei, T. A. (2013). A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydrate Polymers, 97, 253-261.

Ali, M. B. (2014). Secondary metabolites and environmental stress in plants: biosynthesis, regulation, and function. In Physiological Mechanisms and Adaptation Strategies in Plants Under Changing Environment (Ahmad, P. and Wani, M. R.), pp. 55-85. New York: Springer NY.

Athipornchai, A., and Jullapo, N. (2018). Tyrosinase inhibitory and antioxidant activities of Orchid (Dendrobium spp.). South African Journal of Botany, 119, 188-192.

Biswas, R., Chanda, J., Kar, A., and Mukherjee, P. K. (2017). Tyrosinase inhibitory mechanism of betulinic acid from Dillenia indica. Food Chemistry, 232, 689-696.

Chantaraponpan, A., Lakhonphon, K., and Thiabdokmai, S. (2018). Effect of harvesting stages on physical and chemical properties of coriander (Coriandrum sativum L.). Agricultural Science Journal, 49(2) (Supplement), 273-276.

Chaovanalikit, A. (2011). Extraction and analysis of anthocyanin. Srinakharinwirot University Journal of Science and Technology, 3(6), 26-36. (Thai)

Delgado-Vargas, F., Jiménez, A. R., and Paredes-López, O. (2000). Natural pigments: carotenoids, anthocyanins, and betalains-characteristics, biosynthesis, processing, and stability. Critical Reviews in Food Science and Nutrition, 40(3), 173-289.

Gould, K. S., Markham, K. R., Smith, R. H., and Goris, J. J. (2000). Functional role of anthocyanins in the leaves of Quintinia serrate A. Cunn. Journal of Environmental Botany, 51(347), 1107-1115.

Gutiérrez, R. M. P. (2010). Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology. Journal of medicinal plants research, 4(8), 592-638.

Huang, D., Ou, B., and Prior, R. L. (2005). The chemistry behind antioxidant capacity assay, Journal of Agricultural and Food Chemistry, 53, 1841-1856.

Jo, Y. H., Seo, G. U., Yuk, H. G., and Lee, S. C. (2012). Antioxidant and tyrosinase inhibitory activities of methanol extracts from Magnolia denudata and Magnolia denudata var. purpurascens flowers. Food Research International, 47(2), 197-200.

Junka, N., Wongs-Aree, C., and Kanlayanarat, S. (2008). Anthocyanins distributing in flower from 3 native orchids of the VANDEAE Lindley Tribe. Agricultural Sciences, 39(3), 339-342. (Thai)

Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S., and Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47(10), 3954-3962.

Khoo, H. E., Azlan, A., Tang, S. T., and Lim, S. M. (2017). Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food and Nutrition Research, 61(1), 1361779. doi:10.1080/16546628.2017.1361779

Kumar, S. S., and Patra, A. (2018). Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.). Journal of Integrative Medicine, 16, 273-282.

Lee, J., Durst, R. W., and Wrolstad, R. E. (2005). Determination of total monomeric anthocyanin pigment content of fruit juices, beverages nobile, natural colorants, and wines by the pH differential method: Collaborative study. Journal of AOAC International, 88(5), 1269-1278.

Lu, H., Yang, K., Zhan, L., Lu, T., Chen, X., Cai, X., Zhou, C., Li, H., Qian, L., Lv, G., and Chen, S. (2019). Optimization of flavonoid extraction in Dendrobium officinale leaves and their inhibitory effects on tyrosinase activity. International Journal of Analytical Chemistry, 2019(1), 1-10.

Luo, A., and Fan, Y. (2011). In vitro and in vivo antioxidant activity of a water-soluble polysaccharide from Dendrobium fimhriatum Hook. var. oculatum Hook. Molecules Sciences, 12, 4068-4079.

Macwan, C., Patel, H. V. and Andkalia, K. (2010). A comparative evaluation of in vitro antioxidant properties of bamboo Bambusa arundinacea leaves extracts. Journal of Cell and Tissue Research, 10(3), 2413-2418.

Muddathir, A. M., Yamauchi, K., Batubara, I., Mohieldin, E. A. M., and Mitsunaga, T. (2017). Anti-tyrosinase, total phenolic content and antioxidant activity of selected Sudanese medicinal plants. South African Journal of Botany, 109, 9-15.

Nguyen, H. C., Lin, K. H., Huang, M. Y., Yang, C. M., Shih, T. H., Hsiung, T. C., Lin, Y. C., and Tsao, F. C. (2018). Antioxidant activities of the methanol extracts of various parts of Phalaenopsis orchids with white, yellow, and purple flowers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 457-465.

Oki, T., Masuda, M., Furuta, S., Nishiba, Y., Terahara, N., and Suda, A. I. (2002). Involvement of anthocyanins and other phenolic compounds in radical scavenging activity of purple-fleshed sweet potato cultivars. Journal of Food Science, 67, 1752-1756.

Saewan, N., Koysomboon, S., and Chantrapromma, K. (2011). Anti-tyrosinase and anti-cancer activities of flavonoids from Blumea balsamifera DC. Joint FAO/WHO Meeting on Pesticide Residues, 5(6), 1018-1025.

Singleton, V. L., and Rossi, J. R. (1965). Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144-157.

Sompong, R., Siebenhandl-Ehn, S., Linsberger-Martin, G., and Berghofer, E. (2011). Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka. Food Chemistry, 124(1), 132-140.

Sutharut, J., and Sudarat, J. (2012). Total anthocyanin content and antioxidant activity of germinated colored rice. International Food Research Journal, 19(1), 215-221.

Wang, D., Fan, B., Wang, Y., Zhang, L., and Wang, F. (2018). Optimum extraction, characterization, and antioxidant activities of polysaccharides from flowers of Dendrobium devonianum. International Journal of Analytical Chemistry, 2018, https://doi.org/10.1155/2018 /3013497

Wang, G. H., Chen, C. Y., Lin, C. P., Huang, C. L., Lin, C. H., Cheng, C. Y., and Chung, Y. C. (2016). Tyrosinase inhibitory and antioxidant activities of three Bifidobacterium bifidum-fermented herb extracts. Industrial Crops and Products, 89, 376-382.

Yamasaki, H. (1997). A function of colours. Trends in Plant Science, 2, 7-8.

Zengin, G., Uysal, S., Ceylan, R., and Aktumsek, A. (2015). Phenolic constituent, antioxidative and tyrosinase inhibitory activity of Ornithogalum narbonense L. from Turkey: a phytochemical study. Industrial Crops and Products, 70, 1-6.