The Prevalence of Human Intestinal Fluke Infections, Haplorchis taichui, in Thiarid Snails and Cyprinid Fish in Bo Kluea District and Pua District, Nan Province, Thailand
Article Sidebar
Published:
Dec 16, 2016
Keywords:
fish-bornetrematode haplorchis taichui intermediate host prevalence of infection
Main Article Content
Abstract
Traditionally, people in the Nan Province of Thailand eat raw fish, exposing them to a high risk of getting infected by fish-borne trematodes. The monitoring of helminthiasis among those people showed a high rate of infections by the intestinal fluke Haplorchis taichui, suggesting that also an epidemiologic study (of the epidemiology) of the intermediate hosts of this flat worm would be useful. In this study freshwater gastropods of thiarids and cyprinid fish (possible intermediate hosts) were collected around Bo Kluea and Pua District from April 2012 to January 2013. Both snails and fish were identified by morphology and their infections were examined by cercarial shedding and compressing. Cercariae and metacercariae of H. taichui were identified by morphology using 0.5 % neutral red staining. In addition a polymerase chain reaction of the internal transcribed spacer gene (ITS) was applied to the same samples. Among the three thiarid species present were Melanoides tuberculata, Mieniplotia (= Thiara or Plotia) scabra and Tarebia granifera only the latter species was infected with cercariae, with an infection rate or prevalence of infection of 6.61 % (115/1,740). In the 10 species of cyprinid fish (viz. Barbodes schwanenfeldi, Garra cambodgiensis, Hypsibarbus salweenensis, Mystacoleucus marginatus, Osparus pulchellus, Poropuntius deauratus, P. normani, Scaphiodonicthys acanthopterus, Systomus orphoides and S. stolitzkaenus), metacercariae were found in all of them. Interestingly, B. schwanenfeldi, M. marginatus, P. deauratus, P. normani and S. orphoides had very high infection rates. Both morphometric and molecular identification confirmed those cercariae and metacercariae to represent the heterophyid Haplorchis taichui. Therefore, this study showed the high prevalence of this parasite both in its intermediate hosts as well as in humans. In order to prevent human infection, H. taichui has to be controlled.
Downloads
Download data is not yet available.
Article Details
How to Cite
Boonmekam, D., Namchote, S., Nak-ai, W., Glaubrecht, M., & Krailas, D. (2016). The Prevalence of Human Intestinal Fluke Infections, Haplorchis taichui, in Thiarid Snails and Cyprinid Fish in Bo Kluea District and Pua District, Nan Province, Thailand. Science, Engineering and Health Studies, 10(3), 29–37. https://doi.org/10.14456/sustj.2016.10
Section
Research Articles
References
Belizario, V. Y. Jr., de Leon, W. U., Bersabe, M. M. J., Baird, J. K., and Bangs M. J. (2004). A Focus of Human Infection by Haplorchi staichui (Trematoda: Heterophyidae) in the Southern Philippines. Journal of Parasitology, 90(5): 1165–1169.
Boonchot, K., and Wongsawad, C. (2005). A survey of helminthes in cyprinid fish from the Mae Ngad Somboonchon Reservoir, Chiang Mai Province, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 36: 103-107.
Brandt, R. A. M. (1974). The non-marine aquatic molluscain Thailand: German, Walderman Kramer, pp. 19-234.
Chai, J. Y., Murrell K. D., and Lymbery, A.J. (2005). Fish-borne parasitic zoonoses: status and issues.International Journal of Parasitology 35: 1233-1254.D. Boonmekum et al.
Chai, J. Y., Han, E. T., Shin, E. T., Sohn, W. M., Yong, T. S., Eom, K. S., Min, D. Y., Um, J. Y., Park, M. S., Hoang, E. H., Phommasack, B., Insisiengmay, B., Lee, S. H., and Rim, H. J. (2009). High prevalence of Haplorchis taichui, Phaneropsolus molenkampi, and other helminth infection among people in Khammouane province, Lao PDR. Korean Journal of Parasitology, 47(3): 2433-2437.
Chontananarth, T., and Wongsawad, C. (2010). Prevalence of Haplorchis taichui in Field-Collected Snails: A molecular Approach. Korean Journal of Parsitology, 48(4): 343-346.
Chontananarth, T., Wongsawad, C., Chomdej, S., Krailas, D., and Chai, J. (2014). Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCOI).Asian Pacific Journal of Tropical Medicine, 7(6): 446-450.
Chuboon, S., and Wongsawad, C. (2009). Molecular Identification of larval trematode in intermediate hosts from Chiang Mai, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 40(6): 1216- 1220.
De, N. V., Murrel, K. D., and Cong, le. D. (2003). The food-borne trematode zoonoses of Vietnam. Southeast Asian Journal of Tropical Medicine and Public Health, 34(suppl 1): 12-34.
Dung, D. T., Hop N. T., Thaenkham, U., and Waikagul J. (2013). Genetic differences among Vietnamese Haplorchis taichui populations using the COI genetic marker. Journal of Helminthology, 87(1): 66-70.
Kaewkes, S., Elkins, D. B., Sithithaworn, P., and Haswell-Elkins, M. R. (1991). Comparative studies in the morphology of the eggs of Opisthorchis viverriniand Lecithodendriid Trematodes. Southeast Asian Journal of Tropical Medicine and Public Health, 22:623-630.
Krailas, D., Namchote, S., and Rattanathai, P. (2011). Human intestinal flukes Haplorchis taichui and Haplorchis pumilio in their intermediate hosts, freshwater snails of the families Thiaridae and Pachychilidae, in southern Thailand. Zoosystematics and Evolution, 87: 349-360.
Krailas, D., Namchote, S., Koonchornboon, T., Dechruksa, W., and Boonmekam, D. (2014). Trematodes obtained from the thiarid freshwater snail Melanoides tuberculata (Müller, 1774) as vector of humaninfections in Thailand. Zoosystematics and Evolution, 90(1): 57-86.
Kumchoo, K., Wongsawad, C., Chai, J. Y., Vanittanakom, P., and Rojanapaibul, A., (2005) High prevalence of Haplorchis taichui metacercariae in Cyprinid fish from Chiang Mai Province, Thailand Southeast Asian Journal of Tropical Medicine and Public Health, 36(2):451-455.
Le, T. H., De, N. V., Blair, D., Sithithaworn, P., and McManus, D.D. (2006). Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial based PCR for their identification and discrimination. Experimental Parasitology, 112: 109-114.
Macpherson, C. N. L. (2005). Human behavior and the epidemiology of parasitic zoonoses. International Journal of Parasitology, 35: 1319-1331.
Ministry of Public Health (MOPH). (2009). The annual report of helminthiasis control program among the students and youth at the remote area and the Phufa Phattana area under the royal Highness Maha Chakri. Department of Diseases Control, Ministry of Public Health, Bangkok, 143 pp. (in Thai).
Namue, C., Rojanapaibul, A., and Wongsawad, C. (1998). Occurrence of two heterophyid MetacercariaeHaplorchis and Haplorchoides in cyprinid fish in some Districts in Chiang Mai and Lamphun Provinces. Southeast Asian Journal of Tropical Medicine and Public Health 29: 401-405.
Olivier, L. C., and Schneiderman, M. (1956). Method for estimating the density of aquatic snail population. Experimental Parasitology, 5: 109-117.
Radomyos, B., Wongsaroj, T., and Wilairatana, P. (1998). Opisthorchiasis and intestinal fluke infections in northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 29: 123-7.
Rim, H. J., Sohn, W. M., Yong, T. S., Eom, K. S., Chai, J. Y., Min, D. Y., Lee S. H., Hoang, E. H., Phommasack, B., and Insisengmay, S. (2008). Fishborne Trematoda Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean Journal of Parasitology, 46: 253-060.
Sato, M., Thaenkham, U., Dekumyoy, P., and Waikagul, J. (2009). Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions. Acta Tropica, 109: 81-83.
Sohn, W. M., Yong, T. S., Eom, K. S., Min, D. Y., Lee, D., Jung, B.K., Banouvong, V., Insisiengmay, B., Phommasack, B., Rim, H. J., and Chai, J. Y. (2014). Prevalence of Haplorchis taichui among human and fish in Luang Prabang Province, Lao PDR. Acta Tropica, 136: 74-80.
Sukontason, K., Piangjai, S., Muangyimpong, Y., Sukontason, K., Methanitikorn, R., and Chaithong, U. (1999) Prevalence of the matodemetacercariae in cyprinid fish of Ban Pao District, Chiang Mai Province, Northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 30: 365-370.
Sukontason, K. L., Sukontason, K., Boonsriwong, N., and Muangyimpong, Y. (2001) Intensity of the metacercariae in cyprinid fish in Chaing Mai Province, Northern Thailand.Southeast Asian Journal of Tropical Medicine and Public Health, 32 (suppl 2): 214-217.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods.Molecular Biology and Evolution, 28: 2731-2739.
Thaenkham, U., Dekumyoy, P., Komalamisra, C., Sato, M., Dung, D. T., and Waikagul, J. (2010). Systematics of the subfamily Haplorchinae (Trematoda:Heterophyidae), based on nuclear Ribosomal DNA gene and ITS2 region. Parasitology International, 59: 460-465.
Vidthayanon, C. (2005). Freshwater fish in Thailand,Bangkok: Nanmee Book Publishing.
Waikagul, J., Krudsood, S., and Radomyos, P. (2002). A cross-sectopma; study of intestinal parasitic infection among school children in Nan Province, Northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 33: 218-223.
Waikagul, J., Jongsuksantigul, P., Rattanawitoon, U., Radomyos, P., Kojima, S., and Takeuchi, T, (2008). Parasitological Monitoring of Helminth Control Program in Northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 39(6): 1008-1014.
Watthanakulpanich, D., Waikagul, J., Maipanich, W., Nuamtanong, S., Sanguankiat, S., Pubampen, S., Praevanit, R., Mongkhonmu, S., and Nawa, Y.(2010). Haplorchis taichui as a possible etiologic agent of irritable bowel syndrome-like symptoms. Korean Journal of Parasitology, 48(3): 225-9.
WHO (1995). Control of foodborne trematode infections. WHO Technical Report Series, 157.
Wijit, A., Siri, N., and Suya, A. (2009). Monitoring of Helminthiasis Controling Program and Haplorchis taichui infection in Bo KlueaDistrict, Nan Province, Northern of Thailand. Lanna Public Health Journal, 5(3): 318-329 (in Thai).
Yamaguti, S.(1975). Asynoptical review of life histories of digenetic Trematodes of vertebrates, Kyoto: Keigaku Publishing, 590 pp.Silpakorn U Science & Tech J Vol.10(3), 2016D. Boonmekum et al.
Boonchot, K., and Wongsawad, C. (2005). A survey of helminthes in cyprinid fish from the Mae Ngad Somboonchon Reservoir, Chiang Mai Province, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 36: 103-107.
Brandt, R. A. M. (1974). The non-marine aquatic molluscain Thailand: German, Walderman Kramer, pp. 19-234.
Chai, J. Y., Murrell K. D., and Lymbery, A.J. (2005). Fish-borne parasitic zoonoses: status and issues.International Journal of Parasitology 35: 1233-1254.D. Boonmekum et al.
Chai, J. Y., Han, E. T., Shin, E. T., Sohn, W. M., Yong, T. S., Eom, K. S., Min, D. Y., Um, J. Y., Park, M. S., Hoang, E. H., Phommasack, B., Insisiengmay, B., Lee, S. H., and Rim, H. J. (2009). High prevalence of Haplorchis taichui, Phaneropsolus molenkampi, and other helminth infection among people in Khammouane province, Lao PDR. Korean Journal of Parasitology, 47(3): 2433-2437.
Chontananarth, T., and Wongsawad, C. (2010). Prevalence of Haplorchis taichui in Field-Collected Snails: A molecular Approach. Korean Journal of Parsitology, 48(4): 343-346.
Chontananarth, T., Wongsawad, C., Chomdej, S., Krailas, D., and Chai, J. (2014). Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCOI).Asian Pacific Journal of Tropical Medicine, 7(6): 446-450.
Chuboon, S., and Wongsawad, C. (2009). Molecular Identification of larval trematode in intermediate hosts from Chiang Mai, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 40(6): 1216- 1220.
De, N. V., Murrel, K. D., and Cong, le. D. (2003). The food-borne trematode zoonoses of Vietnam. Southeast Asian Journal of Tropical Medicine and Public Health, 34(suppl 1): 12-34.
Dung, D. T., Hop N. T., Thaenkham, U., and Waikagul J. (2013). Genetic differences among Vietnamese Haplorchis taichui populations using the COI genetic marker. Journal of Helminthology, 87(1): 66-70.
Kaewkes, S., Elkins, D. B., Sithithaworn, P., and Haswell-Elkins, M. R. (1991). Comparative studies in the morphology of the eggs of Opisthorchis viverriniand Lecithodendriid Trematodes. Southeast Asian Journal of Tropical Medicine and Public Health, 22:623-630.
Krailas, D., Namchote, S., and Rattanathai, P. (2011). Human intestinal flukes Haplorchis taichui and Haplorchis pumilio in their intermediate hosts, freshwater snails of the families Thiaridae and Pachychilidae, in southern Thailand. Zoosystematics and Evolution, 87: 349-360.
Krailas, D., Namchote, S., Koonchornboon, T., Dechruksa, W., and Boonmekam, D. (2014). Trematodes obtained from the thiarid freshwater snail Melanoides tuberculata (Müller, 1774) as vector of humaninfections in Thailand. Zoosystematics and Evolution, 90(1): 57-86.
Kumchoo, K., Wongsawad, C., Chai, J. Y., Vanittanakom, P., and Rojanapaibul, A., (2005) High prevalence of Haplorchis taichui metacercariae in Cyprinid fish from Chiang Mai Province, Thailand Southeast Asian Journal of Tropical Medicine and Public Health, 36(2):451-455.
Le, T. H., De, N. V., Blair, D., Sithithaworn, P., and McManus, D.D. (2006). Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial based PCR for their identification and discrimination. Experimental Parasitology, 112: 109-114.
Macpherson, C. N. L. (2005). Human behavior and the epidemiology of parasitic zoonoses. International Journal of Parasitology, 35: 1319-1331.
Ministry of Public Health (MOPH). (2009). The annual report of helminthiasis control program among the students and youth at the remote area and the Phufa Phattana area under the royal Highness Maha Chakri. Department of Diseases Control, Ministry of Public Health, Bangkok, 143 pp. (in Thai).
Namue, C., Rojanapaibul, A., and Wongsawad, C. (1998). Occurrence of two heterophyid MetacercariaeHaplorchis and Haplorchoides in cyprinid fish in some Districts in Chiang Mai and Lamphun Provinces. Southeast Asian Journal of Tropical Medicine and Public Health 29: 401-405.
Olivier, L. C., and Schneiderman, M. (1956). Method for estimating the density of aquatic snail population. Experimental Parasitology, 5: 109-117.
Radomyos, B., Wongsaroj, T., and Wilairatana, P. (1998). Opisthorchiasis and intestinal fluke infections in northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 29: 123-7.
Rim, H. J., Sohn, W. M., Yong, T. S., Eom, K. S., Chai, J. Y., Min, D. Y., Lee S. H., Hoang, E. H., Phommasack, B., and Insisengmay, S. (2008). Fishborne Trematoda Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean Journal of Parasitology, 46: 253-060.
Sato, M., Thaenkham, U., Dekumyoy, P., and Waikagul, J. (2009). Discrimination of O. viverrini, C. sinensis, H. pumilio and H. taichui using nuclear DNA-based PCR targeting ribosomal DNA ITS regions. Acta Tropica, 109: 81-83.
Sohn, W. M., Yong, T. S., Eom, K. S., Min, D. Y., Lee, D., Jung, B.K., Banouvong, V., Insisiengmay, B., Phommasack, B., Rim, H. J., and Chai, J. Y. (2014). Prevalence of Haplorchis taichui among human and fish in Luang Prabang Province, Lao PDR. Acta Tropica, 136: 74-80.
Sukontason, K., Piangjai, S., Muangyimpong, Y., Sukontason, K., Methanitikorn, R., and Chaithong, U. (1999) Prevalence of the matodemetacercariae in cyprinid fish of Ban Pao District, Chiang Mai Province, Northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 30: 365-370.
Sukontason, K. L., Sukontason, K., Boonsriwong, N., and Muangyimpong, Y. (2001) Intensity of the metacercariae in cyprinid fish in Chaing Mai Province, Northern Thailand.Southeast Asian Journal of Tropical Medicine and Public Health, 32 (suppl 2): 214-217.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods.Molecular Biology and Evolution, 28: 2731-2739.
Thaenkham, U., Dekumyoy, P., Komalamisra, C., Sato, M., Dung, D. T., and Waikagul, J. (2010). Systematics of the subfamily Haplorchinae (Trematoda:Heterophyidae), based on nuclear Ribosomal DNA gene and ITS2 region. Parasitology International, 59: 460-465.
Vidthayanon, C. (2005). Freshwater fish in Thailand,Bangkok: Nanmee Book Publishing.
Waikagul, J., Krudsood, S., and Radomyos, P. (2002). A cross-sectopma; study of intestinal parasitic infection among school children in Nan Province, Northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 33: 218-223.
Waikagul, J., Jongsuksantigul, P., Rattanawitoon, U., Radomyos, P., Kojima, S., and Takeuchi, T, (2008). Parasitological Monitoring of Helminth Control Program in Northern Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 39(6): 1008-1014.
Watthanakulpanich, D., Waikagul, J., Maipanich, W., Nuamtanong, S., Sanguankiat, S., Pubampen, S., Praevanit, R., Mongkhonmu, S., and Nawa, Y.(2010). Haplorchis taichui as a possible etiologic agent of irritable bowel syndrome-like symptoms. Korean Journal of Parasitology, 48(3): 225-9.
WHO (1995). Control of foodborne trematode infections. WHO Technical Report Series, 157.
Wijit, A., Siri, N., and Suya, A. (2009). Monitoring of Helminthiasis Controling Program and Haplorchis taichui infection in Bo KlueaDistrict, Nan Province, Northern of Thailand. Lanna Public Health Journal, 5(3): 318-329 (in Thai).
Yamaguti, S.(1975). Asynoptical review of life histories of digenetic Trematodes of vertebrates, Kyoto: Keigaku Publishing, 590 pp.Silpakorn U Science & Tech J Vol.10(3), 2016D. Boonmekum et al.