Identification of Apigenin and Luteolin in Artemisia annua L. for the Quality Control

  • Rattana Phadungrakwittaya Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 http://orcid.org/0000-0002-8107-4946
  • Sirikul Chotewuttakorn Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 http://orcid.org/0000-0003-0949-6949
  • Panachai Piwtong Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. http://orcid.org/0000-0003-4067-8353
  • Onusa Thamsermsang Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. http://orcid.org/0000-0002-7566-8238
  • Tawee Laohapand Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. http://orcid.org/0000-0002-9055-2540
  • Pravit Akarasereenont Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Keywords: Artemisia annua L.; TLC fingerprint; apigenin; luteolin

Abstract

Objective: To identify active compounds and establish the chemical fingerprint of Artemisia annua L. for the quality control.
Methods: Thin-layer chromatography (TLC) conditions were developed to screen for 2 common flavonoids (apigenin and luteolin). Three mobile phases were used to isolate these flavonoids in 80% ethanolic extract of A. annua. Hexane : ethyl acetate : acetic acid (31:14:5, v/v) and toluene : 1,4-dioxane : acetic acid (90:25:4, v/v) were used in normal phase TLC (NP-TLC), and 5.5% formic acid in water : methanol (50:50, v/v) were used in reverse phase TLC (RP-TLC). Chromatograms were visualized under visible light after spraying with Fast Blue B Salt. Apigenin and luteolin bands were checked by comparing their Rf values and UV-Vis absorption spectra with reference markers.
Results: Apigenin and luteolin were simultaneously detected with good specificity in RP-TLC condition, while only apigenin was detected in NP-TLC condition. Apigenin band intensity was higher than luteolin band intensity in both conditions.
Conclusion: This knowledge can be applied to the development of quality control assessments to ensure product efficacy and consistency.

Published
2019-05-23
How to Cite
Phadungrakwittaya, R., Chotewuttakorn, S., Piwtong, P., Thamsermsang, O., Laohapand, T., & Akarasereenont, P. (2019). Identification of Apigenin and Luteolin in Artemisia annua L. for the Quality Control. Siriraj Medical Journal, 71(3), 240-245. https://doi.org/10.33192/Smj.2019.37
Section
Original Article