Comparison of Impedance Platelet Count by Sysmex XE-5000 and Beckman Coulter LH 780 with Optical Fluorescent Platelet Count in Thalassemia Patients

Authors

  • Chaicharoen Tantanate

Keywords:

Hematology analyzer, impedance method, optical method, platelet count, thalassemia

Abstract

Background: Red blood cell (RBC) abnormalities are frequently found among thalassemia patients and have been reported to be a cause of spuriously high platelet counts.
Objective: To compare impedance platelet count by Sysmex XE-5000 (PLT-XE) and Beckman Coulter LH 780 (PLT-LH) with optical fluorescent platelet count (PLT-O) in thalassemia patients.
Methods: EDTA blood specimens from 152 thalassemia patients were analyzed by routine mode of PLT-XE and PLT-LH. The PLT-O run on reticulocyte mode of Sysmex XE-5000 was used as a reference method. Spearman’s rank correlation coefficient (rs) and Bland-Altman plot were analyzed.
Results: Correlations between impedance and PLT-O methods were excellent, as demonstrated by rs of 0.95 and 0.97 for PLT-XE and PLT-LH, respectively. However, there was no agreement between impedance and PLT-O methods. There were differences in biases of PLT-XE between thalassemia minor and both thalassemia intermedia and major. Compared to PLT-O, the concordance rates of diagnosis for platelet count abnormality were 94.7% and 96.7% for PLT-XE and PLT-LH, respectively.
Conclusion: Compared to PLT-O, impedance method was associated with spuriously high platelet counts in thalassemia patients. Careful blood smear examination and/or accurate assay should be used in cases suspected of spuriously high platelet count.

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Published

20-09-2017

How to Cite

Tantanate, C. (2017). Comparison of Impedance Platelet Count by Sysmex XE-5000 and Beckman Coulter LH 780 with Optical Fluorescent Platelet Count in Thalassemia Patients. Siriraj Medical Journal, 69(5), 276–282. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/99632

Issue

Vol. 69 No. 5 (2017): September-October

Section

Original Article

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