TY - JOUR AU - Phadungrakwittaya, Rattana AU - Chotewuttakorn, Sirikul AU - Piwtong, Panachai AU - Thamsermsang, Onusa AU - Laohapand, Tawee AU - Akarasereenont, Pravit PY - 2019/05/23 Y2 - 2024/03/28 TI - Identification of Apigenin and Luteolin in Artemisia annua L. for the Quality Control JF - Siriraj Medical Journal JA - Siriraj Med J VL - 71 IS - 3 SE - Original Article DO - 10.33192/Smj.2019.37 UR - https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/190704 SP - 240-245 AB - <p><strong>Objective:</strong> To identify active compounds and establish the chemical fingerprint of <em>Artemisia annua</em> L. for the&nbsp;quality control.<br><strong>Methods:</strong> Thin-layer chromatography (TLC) conditions were developed to screen for 2 common flavonoids (apigenin&nbsp;and luteolin). Three mobile phases were used to isolate these flavonoids in 80% ethanolic extract of <em>A. annua</em>.&nbsp;Hexane : ethyl acetate : acetic acid (31:14:5, v/v) and toluene : 1,4-dioxane : acetic acid (90:25:4, v/v) were used in&nbsp;normal phase TLC (NP-TLC), and 5.5% formic acid in water : methanol (50:50, v/v) were used in reverse phase TLC&nbsp;(RP-TLC). Chromatograms were visualized under visible light after spraying with Fast Blue B Salt. Apigenin and&nbsp;luteolin bands were checked by comparing their Rf values and UV-Vis absorption spectra with reference markers.<br><strong>Results:</strong> Apigenin and luteolin were simultaneously detected with good specificity in RP-TLC condition, while&nbsp;only apigenin was detected in NP-TLC condition. Apigenin band intensity was higher than luteolin band intensity&nbsp;in both conditions.<br><strong>Conclusion:</strong> This knowledge can be applied to the development of quality control assessments to ensure product&nbsp;efficacy and consistency.</p> ER -