The Study of Free radical Scavenging, Total Phenolic Contents and Tyrosinase Inhibition Activity of Crude Extract from Carissa carandas Linn.
Keywords:
Phenolic contents, antioxidant activity, tyrosinase inhibitionAbstract
The present study investigated the changes in total phenolics contents (TPC), tyrosinase inhibition and antioxidant capacity of Carissa carandas Linn. extracts. The effects of different solvent extraction (70% ethanol and 100% ethanol) on the phenolic profile of Carissa carandas Linn. fruit and their tyrosinase inhibition and antioxidant activity were studied. The result showed that the amount of crude extracts from 70% ethanol and ethanol was 5.76 and 2.54% respectively. In fact, the 70% ethanol extract showed the highest content of phenolic compounds at 28.21 mg GAE g–1 crude extract. Results indicated that the ethanol extract exhibited higher DPPH free radical scavenging activity than 70% ethanol extract. ABTS radical scavenging activity and ferric reducing power of 70% ethanol extract showed the highest antioxidant activities of 82.54% and 3494.80 mg g–1 crude extract, respectively. The percentage of tyrosinase inhibition was examined dopachrome method, the 70% ethanol extract exhibited the higher tyrosinase inhibition at 77.65%. The preliminary findings reveal that 7 0 % ethanol extract has a high potential to be used as a natural skin whitening agent in cosmetic products.
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