Efficience of dual-inoculation of Arbuscular mycorrhizal fungi and Phosphate solubilizing bacteria on the growth and tuber inulin content of Jerusalem artichoke (Helianthus tuberosus L.)

Abstract

Jerusalem artichoke (Helianthus tuberosus L.) is an important agricultural crop. Inulin contained in its tuber is considered as a functional food for its positive effects on human health such as preventing obesity, reducing serum cholesterol and so the risk of heart disease. Nowadays, cultivation of Jerusalem artichoke has been performed by
applying chemical and compost. No previous report employing biofertilizer have been conducted. Therefore, the effects of co-inoculation of arbuscular mycorrhizal fungi ( AMF) and phosphate solubilizing bacteria (PSB) on the growth of Jerusalem artichoke cv. JA102xJA89 were conducted in pot trial under non-sterile soil condition. Two AMF
species, Glomus multisubtensum (GM) and Glomus sp.1 (G) and PSB (Klebsiella variicola; KV) were used as inoculums for this study comparing with applied either rock phosphate (RP) or chemical fertilizer (15-15-15). Un-inoculated plant was used as the control. The experimental design was assigned by randomized complete block design ( RCBD) . We
found that the inoculation with G+KV+RP could improve the height, leaf area, tuber fresh weight, weight individual of tuber, inulin accumulation of tuber and total dry mass of plants. While the SPAD chlorophyll meter reading (SCMR) value and number of tuber per plant were no significantly higher than those from un-inoculated control, these finding indicates that the promotion of the growth plant depends by the synergistic effect of dual inoculation with AMF and PSB together with RP.