Efficiency of Actinomyceste for Controlling Fusarium oxysporum f.sp. lycopersici

Abstract

A total of one hundred and twenty-six isolates of Fusarium oxysporum f.sp. lycopersici were successfully isolated from tomato plants showing Fusarium wilt symptoms from commercial fields at Doi Inthanon national park, Chiang Mai. The isolate FolCK_117 was the most virulent isolate due to plant showing wilting or death and selected as representative strain. After that, six strains of soil actinomycetes namely NSP1, NSP2, NSP3, NSP4, NSP5 and NSP6 isolate from the Doi Suthep-Pui national park, Chiang Mai. were tested for ability of growing on glucose yeast malt agar (GYM) mixed with fungicides by the manufacturer recommeded dose include Brodeaux mixture, captan, etridiazole + quintozene, iprodione, mancozeb, metalaxyl and prochloraz. The result showed that all actinomycetes could be able grown on GYM mixed with metalaxyl at recommended concentration (1,000 mg/kg). Moreover, they showed significantly higher on inhibiting the growth of pathogen than actinomycetes growing on GYM. The highest efficacy was given from isolate NSP3, which inhibited mycelial growth and sporulation of fungal pathogen at 84.11 and 94.44%, respectively. In addition, The non-filtrate culture (NF) of actinomyces isolate NSP3 showed the efficiency of 93.89% inhibiting the conidial germination of pathogen at 6 hr. In all experiments, 2 factorial in completely randomized design was conducted.