Hemostatic efficacy of sheep-derived fibrin glue for liver biopsy in swine

Abstract

Fibrin glue has been widely used for controlling hemorrhage and sealing tissue in surgery. The purpose of
this study was to evaluate the hemostatic efficacy of sheep-derived fibrin glue. Six Marino sheep were used to collect
concentrate fibrinogen preparation by the ammonium sulfate precipitation method. Crossbred pigs (n=6) were used to
evaluate hemostatic efficacy by monitoring whole blood clotting time by the glass slide method, bleeding time and
bleeding quantity during liver biopsy. Mean whole blood clotting time on a glass slide started at 201.1±90.47 and
4.43±3.73 seconds, and was completed at 447.83±63.77 and 31.93±4.28 seconds in control and treatment groups
respectively. Two-sited open liver biopsies were performed, each biopsy site was assigned as either control or treatment
groups. One ml of sheep-derived fibrin glue on the bleeding surface was applied at the treatment sited group. Bleeding
quantity was estimated by the increased weight of filter paper after blood straining. Mean bleeding quantity was
0.94±0.38 and 0.1±0.12 g, and mean bleeding time was 175.18±11.80 and 68.08±28.84 seconds in control and treatment
sites, respectively. Whole blood clotting time, bleeding time, and bleeding quantity were significantly less in the
treatment-sited group, compared to the control-sited group (p<0.01). In conclusion sheep-derived fibrin glue could be
applied as an effective hemostatic agent to control hemorrhage after liver biopsy in swine.