การตรวจสอบไวรัส Maize Chlorotic Mottle Virus, Maize Dwarf Mosaic Virus และ Sugarcane Mosaic Virus สาเหตุโรคแห้งตายในข้าวโพดหวาน
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Abstract
The most common viruses infected maize in Thailand are Sugarcane mosaic virus (SCMV), Maize chlorotic mottle virus (MCMV), and Maize dwarf mosaic virus (MDMV), the causal agent of maize lethal necrosis disease. The aims of this study were detection of Sugarcane mosaic virus (SCMV), Maize chlorotic mottle virus (MCMV), and Maize dwarf mosaic virus (MDMV) in the main sweet corn plantation of Thailand by using the indirect ELISA technique. The one hundred and twenty four samples in 3 cultivars of sweet corn such as Hybrix 3, Insee 2 and Wan 54, which were collected from four regions and nine provinces in Thailand, were studied. Results were shown that samples from the central, western, northeastern, and northern regions were infected by both of SCMV and MDMV in the range of average optical density (OD405) 0.107-1.00 with 100 % infectivity. On the other hand, the samples from central and western regions were infected by MCMV in a range of OD405 0.346-1.197 with 31.45 % infectivity. Results from this study were demonstrated that the combination of SCMV and MCMV infection in sweet corn plantation in the central and western regions had more severe viral outbreaks than in the northeastern and northern regions. These results were used as the basic information, that critical epidemiology of these viruses to sweet corn planning, in order to prevent this disease and insect vector or zoning to avoid viral infections to the other sweet corn plantations in Thailand.
Keywords: Sugarcane mosaic virus (SCMV); Maize chlorotic mottle virus (MCMV); Maize dwarf mosaic virus (MDMV); ELISA; detection
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References
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