ความสัมพันธ์เชิงวิวัฒนาการและการจำแนกพืชสกุลขมิ้นโดยใช้ลำดับนิวคลีโอไทด์ที่อยู่ระหว่างยีน trnS(UGA) และ trnfm(CAU)

Main Article Content

พัทธมน แสงอินทร์ ปราณี นางงาม

Abstract

Abstract


The genus Curcuma is perennial herb which is wildly distributed in Thailand especially in the North and Northeast. It has been widely used as medicinal plants, spices and cosmetics. However, the samples identification is quite difficult due to morphological similarity. Recently, the molecular techniques provide valuable tools for identification and accurate detection. Therefore, in this study, sequences data from non-coding region of chloroplast DNA, trnS (UGA)-trnfM (CAU), were used to elucidate phylogenetic relationships and identification within 19 samples of 10 species and 1 unknown sample of Curcuma. The trnS (UGA)-trnfM (CAU) region varied in length from 1,008-1,123 bp. DNA sequences alignment represented different numbers of AT repeats and base substitution which can be used discriminate between C. longa and C. zedoaria. A maximum likelihood analysis was performed on DNA sequences to generate phylogenetic tree which could be divided into two clades. The first clade was Curcuma sp., C. amada, C. comosa, C. longa and C. mangga. The second clade consisted of C. aeruginosa, C. aromatic, C. ecomata, C. rubescens, C. rubrobracteata and C. zedoaria


Keywords: Curcuma; chloroplast DNA; phylogenetic tree

Keywords

Article Details

Section
วิทยาศาสตร์ชีวภาพ
Author Biographies

พัทธมน แสงอินทร์

ภาควิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร ตำบลท่าโพธิ์ อำเภอเมือง จังหวัดพิษณุโลก 65000

ปราณี นางงาม

ภาควิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลัยนเรศวร ตำบลท่าโพธิ์ อำเภอเมือง จังหวัดพิษณุโลก 65000

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