DEFATTED COPRA MEAL HYDROLYSATE AS A NOVEL CANDIDATE FOR PREBIOTIC

พรรณวิภา แพงศรี, สุนีย์ นิธิสินประเสริฐ, อรุณี อิงคกุล, สุทธิพันธ์ แก้วสมพงษ์

Abstract


Mannooligosaccharides (MOS) can be produced from mannan, which is found in many plants such as konjac, ivory nut, locust bean, guar gum, palm kernel, coffee bean and copra meal. In this study, we focused on mannooligosaccharide (MOS) preparation from defatted copra meal by mannanase enzyme from an effective mananase producer, Bacillus circulans NT 6.7. Results showed that MOS could be produced under optimal hydrolysis condition
(20 Units/mL of concentrated enzyme and 50 min of reaction time).  Various sizes of sugar: mannose mannobiose mannotriose mannotetaose mannopentaose and mannohexaose were detected in the Defatted copra meal (DCM) hydrolysate. Moreover, this hydrolysate could promote probiotic bacteria growth as effectively as commercial mannooligosaccharides. Therefore, the results here suggested that the DCM-hydrolysate could be used as prebiotic to improve human and animal health.


Keywords


Mannanase, defatted copra meal hydrolysate, Prebiotic, Mannooligosaccharides

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