พรรณวิภา แพงศรี, สุนีย์ นิธิสินประเสริฐ, อรุณี อิงคกุล, สุทธิพันธ์ แก้วสมพงษ์


Mannooligosaccharides (MOS) can be produced from mannan, which is found in many plants such as konjac, ivory nut, locust bean, guar gum, palm kernel, coffee bean and copra meal. In this study, we focused on mannooligosaccharide (MOS) preparation from defatted copra meal by mannanase enzyme from an effective mananase producer, Bacillus circulans NT 6.7. Results showed that MOS could be produced under optimal hydrolysis condition
(20 Units/mL of concentrated enzyme and 50 min of reaction time).  Various sizes of sugar: mannose mannobiose mannotriose mannotetaose mannopentaose and mannohexaose were detected in the Defatted copra meal (DCM) hydrolysate. Moreover, this hydrolysate could promote probiotic bacteria growth as effectively as commercial mannooligosaccharides. Therefore, the results here suggested that the DCM-hydrolysate could be used as prebiotic to improve human and animal health.


Mannanase, defatted copra meal hydrolysate, Prebiotic, Mannooligosaccharides

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Agnes Weiss, K. J. D. a. W. K. (2005). Comparison of Selective Media for the Enumeration of

Probiotic Enterococci from Animal Feed. Food Technol. Biotechnol. 43(2): 147-155.

AOAC. (1990). Official methods of analysis of the association of official analytical : 15th ed

chemists Gaithersburg, MD : AOAC Inc. press.

Feng.Y.Y. , Z. M. H., S.L. Ong, J.Y. Hu, Z.G. Zhang & Ng. W.J. (2003). Optimization of agitation,

aeration, and temperature conditions for maximum β-mannanase production.

Enzyme and Microbial Technology. 25: 1143-1146.

Gibson, G. R., & Roberfroid M.B. (1995). Dietary modulation of the human colonic microbiota:

Introducing the concept of prebiotics. Journal of Nutrition. 125: 1401-1402.

Gibson, G. R., Probert, H. M., Van Loo, J., Rastall, R. A., & Roberfroid M.B. (2004). Dietary

modulation of the human colonic microbiota: Updating the concept of prebiotics.

Nutrition Research Reviews .17: 259-275.

Jiang Z, W. Y., Li D, Li L, Chai P and Kusakabe I. (2006). High-level production, purification and

characterization of a thermostable β-mannanases from the newly isolated Bacillus

subtilis WY34. Carbohydr. Polym. 66: 88-96.

Lin, T.C. & Chen C. (2004). Enhanced mannanase production by submerged culture of

Aspergillus niger NCH-189 using defatted copra based media. Process Biochemistry.

(9): 1103-1109.

Line, J. E. S. B., N.A. Cox & Stern N.J. (1997). Yeast treatment to is reduce Salmonella and

Campylobacter, populations associated with broiler chickens subjected to transport

stress. Poultry Sci. 76: 1227-1231.

Macfaddin, J. F. (2000). Biochemical tests for identification of medical bacteria: 3rd ed

Philadelphia : Lippincott Williams & Wilkins.

Manning, T. G., & Gibson GR. (2004). Prebiotics. Best Practice & Research Clinical

Gastroenterology. 18(2): 287-298.

Miller, G. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal

Chem.31: 426-428.

Mohammad Z.H, J. Abe, & Hizukuri S. (1996). Multiple forms of β-mannanase from Bacillus sp.

KK01. Enzyme Microb Technol. 18: 95-98.

Nitisinprasert S, N. V., Bunyun P, Sukyai P, Doi K, & Sonomoto K. (2000). Screening and

identification of effective thermotolerantlactic acid bacteria producing antimicrobial

activity against Escherichia coli and Salmonella sp. resistant to antibiotics. Kasetsart J

(Nat Sci) 34: 387-400.

Phothichitto, K., Nitisinprasert, S. & Keawsompong S. (2006). Isolation, Screening and

Identification of Mannanase Producing Microorganisms. Kasetsart J. (Nat. Sci.). 40:


Sachslehner, A., Foidl,G. , Foidl,N. , bitz,G.G., and Haltrich D. (2000). Hydrolysis of isolated

coffee mannan and coffee extract by mannanases of Sclerotium rolfsii. Journal of

Biotechnology.80: 127-134.

Titapoka , S., Keawsompong,S., D. Haltrich. & Nitisinprasert S. (2008). Selection and

characterization of mannanase-producing bacteriauseful for the formation of prebiotic

manno-oligosaccharidesfrom copra meal. World J Microbiol Biotechnol .24: 1425-1433.

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