Keratinase Fermentation by Bacillus licheniformis KUB-K0006

Warintorn Eaksuree, Akkharadet Prachayakitti, Rutjawate Taharnklaew, Dietmar Haltrich, Sunee Nitisinprasert, Suttipun Keawsompong


              Keratinase fermentation by Bacillus licheniformis KUB-K0006 was studied at pilot scale. The maximum activity (5.03 U/mL) and productivity (0.21 U/L h) were obtained at 5% (v/v) inoculum, 1.0 vvm aeration rate, without pH control (7.5 initial pH), agitation speed 300 rpm,  at the temperature of 37°C for 24 h. During fermentation, controlled pH 7.5 gave similar enzyme activity to without controlled pH. This condition achieved its maximum productivity for keratinase production in 3 L fermentor. Fermentation scale-up was also studied by varying fermentor size at the volume of 19.5, 70 and 300 L. The profile of pH, growth and keratinase activity were similar in all fermentor sizes. The result suggested that the characteristics of the fermentors had little effect on keratinase production patterns in B. licheniformis KUB-K0006.


Keratinase Production; Bacillus; Scale up; Feather

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