ISOLATION AND SCREENING OF MANNANASE PRODUCING BACTERIA FROM MANNAN WASTE

Phanwipa Pangsri

Abstract


          Mannanase is the most important enzyme for the hydrolysis of mannan, which is produced by microorganisms, including bacteria and fungi. Mannanase could be used as valuable food, its application for mannooligosaccharides preparation as prebiotic. This study focus on the isolation and screening an effective strain of mannanase producing bacteria. The result found that 2 isolates showing mannanase production were isolated from soil samples, which compose of waste from mannan. Isolate P2-5 and P3-1 showed high ratio of clear zone diameter to colony diameter 1.75 and 1.50, respectively. Isolate P2-5 and P3-1 were identified by 16S rDNA gene sequencing, biochemical test and morphology. The results of bacterial identification revealed P2-5 and P3-1 as Bacillus subtilis, which were 99% identity. The both isolates were able to ferment some of carbohydrates such as D-glucose D-mannitol
D-mannose Myo-inositol D-ribose and D-trehalose. Bacillus species are an important source of mannanase enzymes production. Therefore, the both isolates will be used for mannanase enzyme production and its application for various industrials and  mannooligosaccharides preparation.


Keywords


Isolation; Mannanase; Mannan Waste; Bacillus subtilis

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