ISOLATION AND SCREENING OF MANNANASE PRODUCING BACTERIA FROM MANNAN WASTE

Main Article Content

Phanwipa Pangsri

Abstract

          Mannanase is the most important enzyme for the hydrolysis of mannan, which is produced by microorganisms, including bacteria and fungi. Mannanase could be used as valuable food, its application for mannooligosaccharides preparation as prebiotic. This study focus on the isolation and screening an effective strain of mannanase producing bacteria. The result found that 2 isolates showing mannanase production were isolated from soil samples, which compose of waste from mannan. Isolate P2-5 and P3-1 showed high ratio of clear zone diameter to colony diameter 1.75 and 1.50, respectively. Isolate P2-5 and P3-1 were identified by 16S rDNA gene sequencing, biochemical test and morphology. The results of bacterial identification revealed P2-5 and P3-1 as Bacillus subtilis, which were 99% identity. The both isolates were able to ferment some of carbohydrates such as D-glucose D-mannitol
D-mannose Myo-inositol D-ribose and D-trehalose. Bacillus species are an important source of mannanase enzymes production. Therefore, the both isolates will be used for mannanase enzyme production and its application for various industrials and  mannooligosaccharides preparation.

Article Details

Section
Research Articles
Author Biography

Phanwipa Pangsri

Biotechnology Program, Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Pathumthani, Thailand

References

Abe J, Hossain ZM, Hizukuri S. (1994). Isolation of beta-mannanase producing microorganisms.
Journal of Fermentation Bioengineering. 3:259–261.
Chauhan, P. S., N. Puri, P. Sharma, and N. Gupta. (2012). Mannanases: microbial
sources, production, properties and potential biotechnological applications.
Applied Microbiology biotechnology. 93(5): 1817-1830.
Emi, S., Fukumoto, J. and Yamamoto, T. (1972). Crystallization and some properties
of mannanase. Agricultural and Biological Chemistry. 36 : 991-1001.
Holt, J. G., Krieg, N. R., Sneath, P.H. A., Staley, J. T., and Williams, S.T. (1994). Bergey’s manual
of determinative bacteriology. 9thed. Williams & Wilkins USA.
McCleary, B.V. (1988). Beta -D-Mannanase. Methods in Enzymology. 160, 596-610.
Mohammad Z.H, J. Abe, and S. Hizukuri. (1996). Multiple forms of beta-mannanase
from Bacillus sp. KK01. Enzyme Microbial Technology. 18: 95-98.
Oda, Y. and Tonomura, K. (1996). Characterization of beta-mannanase and beta-
mannosidase secreted from yeast Trichosporon cutaneum JCM 2947. Letters in
Applied Microbiology. 22: 173-178.
Pangsri, P., Nitisinprasert,S., Ingkakul,A., and S. Keawsompong. (2015a). Defatted Copra meal
Hydrolysate as Novel Candidate for Prebiotic. VRU Research and Development
Journal. Vol 10 No. 3 :1-10.
Pangsri, P., Piwpankaew, Y., Ingkakul, A., Nitisinprasert, S., & Keawsompong, S. (2015b).
Characterization of mannanase from Bacillus circulans NT 6.7 and its application in
mannooligosaccharides preparation as prebiotic. SpringerPlus, 4, 771. http://doi.org/
10.1186/s40064-015-1565-7.
Phothichitto,K., S. Nithisinprasert and S. Keawsompong. (2006). Isolation, Screening and
Identification of Mannanase Producing Microorganisms. Kasetsart Journal (Nat. Sci.) 40 (Suppl.) : 26-38.
Rattanasuk,S. and Ketudat-Cairns,M. (2009). Chryseobacterium indologenes, novel mannanase-
producing bacteria. Songklanakarin Journal Science Technology. 31 (4) : 395-399.
Ratto, M. and Poutanen, K. (1988). Production of mannan-degrading enzymes. Biotechnology
Letters. 10, 661-664.
Sachslehner, A., Foidl,G. , Foidl,N. , bitz,G.G., and Haltrich D. (2000). Hydrolysis of isolated
coffee mannan and coffee extract by mannanases of Sclerotium rolfsii. Journal of
Biotechnology. 80: 127-134.
Soumya, R. S., and E. T. Abraham. (2010). Isolation of beta-mannanase from Cocos nucifera
Linn haustorium and its application in the depolymerization of beta-(1,4)-linked
D-mannans. International Journal Food Science Nutrition. 61(3): 272-281.
Takahashi, R., Kusakabe, I. and Kobayashi, H. (1984). Purification and some properties of
mannanase from Streptomyces sp. Agricultural and Biological Chemistry.48:
2189-2195.
Titapoka. S., S. Keawsompong, D. Haltrich and S. Nitisinprasert. (2008). Selection and
characterization of mannanase-producing bacteria useful for the formation of
prebiotic manno-oligosaccharides from copra meal. 24 (8) : 1425–1433 World
Journal Microbiology Biotechnology. DOI 10.1007/s11274-007-9627-9.