CHARACTERIZATION OF FLOUR, STARCH AND PROTEIN FROM WHITE AND BLACK ADLAY CULTIVARS
Adlay or Job’s tears (Coix lachryma-jobi L.) from white (WA) and black (BA) adlay cultivars were used to study the physical, chemical and physicochemical properties of adlay flour (F), starch (S) and protein (P). Whiteness indexes of adlay flour (WAF, BAF) were lower than adlay starch (WAS, BAS) and protein (WAP, BAP). In addition, the whiteness index of flour and protein from BA was lower than that of WA. While morphology of adlay starch, flour and protein from both cultivars was similar; however, the size of protein bodies was slightly different. Additionally, the molecular weight of protein in WAP and BAP has similar pattern which consisted of the α-, β- and γ-coixin. In addition, adlay flours (WAF, BAF) composed of carbohydrate (79.74-81.71%), protein (13.28-14.88%) and lipid (3.21-3.53%). Moreover, the protein content of flour, protein and starch from black adlay cultivar seemed to be higher than that of white adlay cultivar. Furthermore, the amylose content of BAS (29.22%) was significantly higher than that of WAS (11.55%). Additionally, the pasting viscosity of adlay starches (WAS, BAS) was higher than adlay flours (WAF, BAF). In addition, the WAS and WAF had peak viscosity and breakdown value higher than BAS and BAF. However, the BAS had final viscosity and setback value higher than WAS.
AOAC (2000). Official Methods of Analysis (20th ed). Virginia: The Association of Official Analytical Chemists.
Copeland, L., Blazek, J., Salman, H. & Tang, M.C. (2009). Form and functionality of starch. Food Hydrocolloids, 23, 1527-1534.
Fitzgerald, M.A., Martin, M., Ward, R.M., Park, W.D. & Shead, H.J. (2003). Viscosity of rice flour: a rheological and biological study. Journal of Agricultural and Food Chemistry, 51, 2295-2299.
Han, X.-Z., Campanella, O.H., Guan, H., Keeling, P.L. & Hamaker, B.R. (2002). Influence of maize starch granule-associated protein on the rheological properties of starch pastes. Part I. Large deformation measurements of pastes properties. Carbohydrate Polymers, 49, 315-321.
Han, X.Z. & Hamaker, B.R. (2002). Association of starch granule proteins with starch ghosts and remnants revealed by confocal laser scanning microscopy. Cereal Chemistry, 79, 892-896.
Juliano, B.O. (1971). A simplified assay for milled-rice amylose. Cereal Science Today, 16 (10), 334-340.
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 681-685.
Li, J. & Corke, H. (1999). Physicochemical properties of normal and waxy Job’s tears (Coix lachryma-jobi L.) starch. Cereal Chemistry, 76, 413-416.
Li, E., Dhital, S. & Hasjim, J. (2014). Effects of grain milling on starch structures and flour/starch properties. Starch - Stärke, 66, 15-27.
Li, M. & Lee, T.C. (1996). Effect of cysteine on the functional properties and microstructures of wheat flour extrudates. Journal of Agricultural and Food Chemistry, 44, 1871-1880.
Mishra, S. & Rai, T. (2006). Morphology and functional properties of corn, potato and tapioca starches. Food Hydrocolloids, 20, 557-566.
Olayinka, O.O., Adebowale, K.O. & Olu-Owolabi, I.B. (2013). Physicochemical properties, morphological and X-ray pattern of chemically modified white sorghum starch. Journal of Food Science and Technology, 50 (1), 515-519.
Ottoboni, L.M. M., Leite, A., Targon, M.L.N., Crozier, A. & Arruda, P. (1990). Characterization of the storage protein in seed of Coix lacryma-jobi var. adlay. Journal of Agricultural and Food Chemistry, 38, 631-635.
Parris, N. & L.C. Dickey (2001) Extraction and solubility characteristics of zein proteins from dry-milled corn. Journal of Agricultural and Food Chemistry, 49, 3757-3760.
Pronkitprasan, T. (1987). Job’s tears: some properties and products. M.S. Thesis. Kasetsart University.
Sasaki, T., Yasui, T. & Matsuki, J. (2000). Effect of amylose content on gelatinization, retrogradation and pasting properties of starches from waxy and nonwaxy wheat and their F1 seeds. Cereal Chemistry, 77 (1), 58-63.
Singh, N., Singh, J., Kaur, L., Sodhi, N.S. & Gill, B.S. (2003). Morphological, thermal and rheological properties of starches from different botanical sources. Food Chemistry, 81, 219-231.
Sira, E.E.P. & Amaiz, M.L. (2004). A laboratory scale method for isolation of starch from pigmented sorghum. Journal of Food Engineering, 64, 515-519.
Tatham, A.S., Fido, R.J., Moore, C.M., Kasarda, D.D., Kuzmicky, D.D., Keen, J.N. & Shewry, P.R. (1996). Characterisation of major prolamins of Tef (Eragrostis tef) and Finger millet (Eleusine coracana). Journal of Cereal Science, 24, 65-71.
Taylor, J., Taylor, J.R.N., Dutton, M.F. & Kock, S.D. (2005). Identification of kafirin film casting solvents. Food Chemistry, 90, 401-408.
Whistler, R.L. & BeMiller, J.N. (1999). Carbohydrate Chemistry for Food Scientists (2nd ed). Minnesota: American Association of Cereal Chemists.
Yao, H.-T, Lin, J.-H., Chiang, M.-T., Chiang, W., Luo, M.-N. & Lii, C.-K. (2011). Suppressive effect of the ethanolic extract of adlay bran on Cytochrome P-450 enzymes in rat liver and lungs. Journal of Agricultural and Food Chemistry, 59, 4306-4314.
Yu, F., Gao, J., Zeng, Y. & Liu, C.-X. (2011). Effects of adlay seed oil on blood lipids and antioxidant capacity in hyperlipidemic rats. Journal of the Science of Food and Agriculture, 91, 1843-1848.
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